Search Results - "Breitling, Reinhard"

Insights on a putative aminoacyl-tRNA-protein transferase of Leishmania major by Sharma, Rohit, Terrão, Monica Cristina, Castro, Felipe Freitas, Breitling, Reinhard, Faça, Vitor, Oliveira, Eduardo Brandt, Cruz, Angela Kaysel

“…The N-end rule pathway leads to regulated proteolysis as an adaptive response to external stress and is ubiquitous from bacteria to mammals. In this study, we…”
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Relationships between Cargo, Cell Penetrating Peptides and Cell Type for Uptake of Non-Covalent Complexes into Live Cells by Keller, Andrea-Anneliese, Mussbach, Franziska, Breitling, Reinhard, Hemmerich, Peter, Schaefer, Buerk, Lorkowski, Stefan, Reissmann, Siegmund

“…Modulating signaling pathways for research and therapy requires either suppression or expression of selected genes or internalization of proteins such as…”
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The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic Sec61p complex by Meyer, T H, Ménétret, J F, Breitling, R, Miller, K R, Akey, C W, Rapoport, T A

“…The SecYEG complex is a major component of the protein translocation apparatus in the cytoplasmic membrane of bacteria. We have purified a translocationally…”
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Transduction and transfection of difficult‐to‐transfect cells: Systematic attempts for the transfection of protozoa Leishmania by Keller, Andrea‐Anneliese, Scheiding, Berith, Breitling, Reinhard, Licht, Andreas, Hemmerich, Peter, Lorkowski, Stefan, Reissmann, Siegmund

“…Cell‐penetrating peptides (CPPs) are used to internalize different cargoes, including DNA, into live mammalian and plant cells. Despite many cells being easily…”
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Transduction of Proteins into Leishmania Tarentolae by Formation of Non-Covalent Complexes With Cell-Penetrating Peptides by Keller, Andrea-Anneliese, Breitling, Reinhard, Hemmerich, Peter, Kappe, Katarina, Braun, Maria, Wittig, Berith, Schaefer, Buerk, Lorkowski, Stefan, Reissmann, Siegmund

“…ABSTRACT Cell‐penetrating peptides (CPPs) are used to transport peptides, proteins, different types of ribonucleic acids (or mimics of these molecules), and…”
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Artificial linear episome-based protein expression system for protozoon Leishmania tarentolae by Kushnir, Susanna, Cirstea, Ion Cristian, Basiliya, Lyudmyla, Lupilova, Nataliya, Breitling, Reinhard, Alexandrov, Kirill

“…[Display omitted] ▶ The protozoon Leishmania tarentolae was developed as a host for recombinant protein production. ▶ We present a new type of expression…”
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Chemical and enzymatic characterization of recombinant rabbit muscle pyruvate kinase by Boehme, Christian, Bieber, Frank, Linnemann, Julia, Breitling, Reinhard, Lorkowski, Stefan, Reissmann, Siegmund

“…The stepwise synthesis of thymidine triphosphate (TTP) requires a kinase for phosphorylation in the last step. Because pyruvate kinase (PK) using…”
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Development of an inducible protein expression system based on the protozoan host Leishmania tarentolae by Kushnir, Susanna, Gase, Klaus, Breitling, Reinhard, Alexandrov, Kirill

“…Production of functional eukaryotic proteins in recombinant form is a bottle-neck in various post-genomic applications and in life science in general. At least…”
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Characterization of the growth behavior of Leishmania tarentolae - a new expression system for recombinant proteins by Fritsche, Claudia, Sitz, Mandy, Weiland, Norman, Breitling, Reinhard, Pohl, Hans-Dieter

“…Biotechnological production of recombinant proteins for human therapy requires a cultivation of the host organism in a nutrient medium free of animal…”
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Non-pathogenic trypanosomatid protozoa as a platform for protein research and production by Breitling, Reinhard, Klingner, Susanne, Callewaert, Nico, Pietrucha, Regina, Geyer, Anett, Ehrlich, Gunter, Hartung, Regina, Müller, Angelika, Contreras, Roland, Beverley, Stephen M, Alexandrov, Kirill

“…All currently existing eukaryotic protein expression systems are based on autonomous life forms. To exploit the potential practical benefits associated with…”
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Construction and analysis of Leishmania tarentolae transgenic strains free of selection markers by Mureev, Sergei, Kushnir, Susanna, Kolesnikov, Alexander A., Breitling, Reinhard, Alexandrov, Kirill

“…The trypanosomatid protozoan Leishmania tarentolae has been extensively used as a model system for studying causative agents of several tropical diseases and…”
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Purification and crystallization of human Cu/Zn superoxide dismutase recombinantly produced in the protozoan Leishmania tarentolae by Gazdag, Emerich Mihai, Cirstea, Ion Cristian, Breitling, Reinhard, Lukeš, Julius, Blankenfeldt, Wulf, Alexandrov, Kirill

“…The rapid and inexpensive production of high‐quality eukaryotic proteins in recombinant form still remains a challenge in structural biology. Here, a…”
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SecA is required for membrane targeting of the cell division protein DivIVA in vivo by Halbedel, Sven, Kawai, Maki, Breitling, Reinhard, Hamoen, Leendert W

“…The conserved protein DivIVA is involved in different morphogenetic processes in Gram-positive bacteria. In Bacillus subtilis, the protein localizes to the…”
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Relationships between Cargo, Cell Penetrating Peptidesand Cell Type for Uptake of Non-Covalent Complexes into Live Cells by Keller, Andrea-Anneliese, Mussbach, Franziska, Breitling, Reinhard, Hemmerich, Peter, Schaefer, Buerk, Lorkowski, Stefan, Reissmann, Siegmund

“…Modulating signaling pathways for research and therapy requires either suppression or expression of selected genes or internalization of proteins such as…”
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Isotopic labeling of recombinant proteins expressed in the protozoan host Leishmania tarentolae by Niculae, Anca, Bayer, Peter, Cirstea, Ion, Bergbrede, Tim, Pietrucha, Regina, Gruen, Mathias, Breitling, Reinhard, Alexandrov, Kirill

“…Isotope labeling of recombinant proteins is a prerequisite for application of nuclear magnetic resonance spectroscopy (NMR) for the characterization of the…”
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Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5′ sequence context by Lukeš, Julius, Paris, Zdeněk, Regmi, Sandesh, Breitling, Reinhard, Mureev, Sergey, Kushnir, Susanna, Pyatkov, Konstantin, Jirků, Milan, Alexandrov, Kirill A.

“…To investigate the influence of sequence context of translation initiation codon on translation efficiency in Kinetoplastida, we constructed a library of…”
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A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae by Foldynová-Trantírková, Silvie, Matulová, Jana, Dötsch, Volker, Löhr, Frank, Cirstea, Ion, Alexandov, Kirill, Breitling, Reinhard, Lukeš, Julius, Trantírek, Lukáš

“…We report a cost efficient approach for amino-acid-type selective isotope labeling of proteins expressed in Leishmania tarentolae. The method provides an…”
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Rapid Production of Functionalized Recombinant Proteins:  Marrying Ligation Independent Cloning and in Vitro Protein Ligation by Kushnir, Susanna, Marsac, Yoann, Breitling, Reinhard, Granovsky, Igor, Brok-Volchanskaya, Vera, Goody, Roger S, Becker, Christian F. W, Alexandrov, Kirill

“…Functional genomics and proteomics have been very active fields since the sequencing of several genomes was completed. To assign a physiological role to the…”
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A genetically encodable microtag for chemo-enzymatic derivatization and purification of recombinant proteins by Dursina, Beatrice-Elena, Reents, Reinhard, Niculae, Anca, Veligodsky, Alexei, Breitling, Reinhard, Pyatkov, Konstantin, Waldmann, Herbert, Goody, Roger S., Alexandrov, Kirill

“…Efficient separation of recombinant polypeptides from proteins of the expression host and their subsequent derivatisation with functional chemical groups is…”
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Modulation of the spc operon affects growth and protein secretion in Bacillus subtilis by Breitling, R, Schlott, B, Behnke, D

“…A proximal segment of B. subtilis secY gene was placed under the control of the inducible spac promoter/Lac repressor system. This fusion was integrated into…”
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