Search Results - "Braunfeld, Michael B"

Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM by Li, Xueming, Mooney, Paul, Zheng, Shawn, Booth, Christopher R, Braunfeld, Michael B, Gubbens, Sander, Agard, David A, Cheng, Yifan

“…The combination of a direct electron-detection camera that can count individual electrons and an algorithm for correcting for beam-induced motion in cryo-EM…”
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UCSF tomography: An integrated software suite for real-time electron microscopic tomographic data collection, alignment, and reconstruction by Zheng, Shawn Q., Keszthelyi, Bettina, Branlund, Eric, Lyle, John M., Braunfeld, Michael B., Sedat, John W., Agard, David A.

“…A real-time alignment and reconstruction scheme for electron microscopic tomography (EMT) has been developed and integrated within our UCSF tomography data…”
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three-dimensional structure of in vitro reconstituted Xenopus laevis chromosomes by EM tomography by König, Peter, Braunfeld, Michael B, Sedat, John W, Agard, David A

“…We have studied the in vitro reconstitution of sperm nuclei and small DNA templates to mitotic chromatin in Xenopus laevis egg extracts by three-dimensional…”
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3D Nanometer-Scale Study of Coexisting Bicontinuous Morphologies in a Block Copolymer/Homopolymer Blend by Jinnai, Hiroshi, Hasegawa, Hirokazu, Nishikawa, Yukihiro, Sevink, G. J. Agur, Braunfeld, Michael B., Agard, David A., Spontak, Richard J.

“…Coexisting bicontinuous morphologies, one ordered and one disordered, are investigated in a macrophase‐separated poly(styrene‐block‐isoprene) diblock…”
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Nanofibrillar Networks in Poly(ethyl methacrylate) and Its Silica Nanocomposites by Wilder, Elizabeth A, Braunfeld, Michael B, Jinnai, Hiroshi, Hall, Carol K, Agard, David A, Spontak, Richard J

“…Recent advances in polymer materials design seek to incorporate functionality, enhance existing properties, and reduce weight without compromising mechanical…”
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Automated acquisition of electron microscopic random conical tilt sets by Zheng, Shawn Q., Kollman, Justin M., Braunfeld, Michael B., Sedat, John W., Agard, David A.

“…Single particle reconstruction using the random conical tilt data collection geometry is a robust method for the initial determination of macromolecular…”
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An improved strategy for automated electron microscopic tomography by Zheng, Qingxiong S., Braunfeld, Michael B., Sedat, John W., Agard, David A.

“…A prediction-based scheme is proposed and implemented for automated electron microscopic tomography. By assuming that the sample follows a simple geometric…”
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Structure of the gamma-tubulin ring complex: a template for microtubule nucleation by Moritz, M, Braunfeld, M B, Guénebaut, V, Heuser, J, Agard, D A

“…The gamma-tubulin ring complex (gammaTuRC) is a protein complex of relative molecular mass approximately 2.2 x 10(6) that nucleates microtubules at the…”
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Electron energy filtering significantly improves amplitude contrast of frozen-hydrated protein at 300 kV by Yonekura, Koji, Braunfeld, Michael B., Maki-Yonekura, Saori, Agard, David A.

“…The amplitude contrast of frozen-hydrated biological samples was measured using the bacterial flagellar filament embedded in vitreous ice at an accelerating…”
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A distributed multi-GPU system for high speed electron microscopic tomographic reconstruction by Zheng, Shawn Q., Branlund, Eric, Kesthelyi, Bettina, Braunfeld, Michael B., Cheng, Yifan, Sedat, John W., Agard, David A.

“…Full resolution electron microscopic tomographic (EMT) reconstruction of large-scale tilt series requires significant computing power. The desire to perform…”
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Dual-axis target mapping and automated sequential acquisition of dual-axis EM tomographic data by Zheng, Shawn Q., Matsuda, Atsushi, Braunfeld, Michael B., Sedat, John W., Agard, David A.

“…Dual-axis electron microscopic tomography minimizes the missing wedge-induced resolution loss by taking two complementary tilt data sets of the same target…”
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Microtubule nucleation by γ-tubulin-containing rings in the centrosome by Moritz, Michelle, Braunfeld, Michael B, Sedat, John W, Alberts, Bruce, Agard, David A

“…The microtubule cytoskeleton of animal cells does not assemble spontaneously, but instead requires the centrosome. This organelle consists of a pair of…”
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Architecture-Induced Phase Immiscibility in a Diblock/Multiblock Copolymer Blend by Spontak, Richard J, Fung, Jennifer C, Braunfeld, Michael B, Sedat, John W, Agard, David A, Ashraf, Arman, Smith, Steven D

“…Ordered diblock copolymer blends have recently become the subject of tremendous research interest since they can be used to elucidate the intramicrodomain…”
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Spindle pole body duplication in fission yeast occurs at the G1/S boundary but maturation is blocked until exit from S by an event downstream of cdc10 by Uzawa, Satoru, Li, Fei, Jin, Ye, McDonald, Kent L, Braunfeld, Michael B, Agard, David A, Cande, W Zacheus

“…The regulation and timing of spindle pole body (SPB) duplication and maturation in fission yeast was examined by transmission electron microscopy. When cells…”
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Tunable Instability Mechanisms of Polymer Thin Films by Molecular Self-Assembly by Wei, Bin, Lam, Peter G, Braunfeld, Michael B, Agard, David A, Genzer, Jan, Spontak, Richard J

“…Incorporation of a block copolymer into a thin polymer film is observed to alter both the rate and mechanism by which the film dewets from an immiscible…”
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Three-dimensional structural characterization of centrosomes from early Drosophila embryos by Moritz, Michelle, Braunfeld, Michael B., Fung, Jennifer C., Sedat, John W., Alberts, Bruce M., Agard, David A.

“…An understanding of the mechanism and structure of microtubule (MT)-nucleating sites within the pericentriolar material (PCM) of the centrosome has been…”
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Phase Behavior of Ordered Diblock Copolymer Blends:  Effect of Compositional Heterogeneity by Spontak, Richard J, Fung, Jennifer C, Braunfeld, Michael B, Sedat, John W, Agard, David A, Kane, Lisaleigh, Smith, Steven D, Satkowski, Michael M, Ashraf, Arman, Hajduk, Damian A, Gruner, Sol M

“…Diblock copolymers order into a variety of periodic morphologies when the constituent blocks are sufficiently incompatible. Previous studies have demonstrated…”
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Large-scale chromatin structural domains within mitotic and interphase chromosomes in vivo and in vitro by Belmont, A.S, Braunfeld, M.B, Sedat, J.W, Agard, D.A

“…Higher-order chromatin structural domains approximately 130 nm in width are observed as prominent components of both Drosophila melanogaster and human mitotic…”
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Identification of prion amyloid filaments in scrapie-infected brain by DeArmond, S J, McKinley, M P, Barry, R A, Braunfeld, M B, McColloch, J R, Prusiner, S B

“…Extracellular collections of abnormal filaments composed of prion proteins have been identified in the brains of scrapie-infected hamsters using immunoelectron…”
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Electron counting and beam-induced motion correction enable near atomic resolution single particle cryoEM by Li, Xueming, Mooney, Paul, Zheng, Shawn, Booth, Chris, Braunfeld, Michael B., Gubbens, Sander, Agard, David A., Cheng, Yifan

“…In recent work with large high symmetry viruses, single particle electron cryomicroscopy (cryoEM) has reached the milestone of determining near atomic…”
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