A plant-produced plague vaccine candidate confers protection to monkeys

Abstract Production of vaccine antigens in plants has received considerable attention over the last decade. However, despite many antigens being expressed in plant systems, and promising efficacy data with rodent models, few vaccine candidates have advanced into studies in non-human primates or huma...

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Bibliographic Details
Published in:	Vaccine Vol. 25; no. 16; pp. 3014 - 3017
Main Authors:	Mett, Vadim, Lyons, Jarred, Musiychuk, Konstantin, Chichester, Jessica A, Brasil, Trevor, Couch, Ronald, Sherwood, Robert, Palmer, Gene A, Streatfield, Stephen J, Yusibov, Vidadi
Format:	Journal Article Conference Proceeding
Language:	English
Published:	Oxford Elsevier Ltd 20-04-2007 Elsevier Elsevier Limited
Subjects:	Allergy and Immunology Animals Antigens Antigens, Bacterial - biosynthesis Antigens, Bacterial - metabolism Applied microbiology Bacterial diseases Bacterial Proteins - biosynthesis Bacteriology Biological and medical sciences Clostridium thermocellum Cynomolgus Disease Models, Animal Fundamental and applied biological sciences. Psychology Genetic Engineering Genetic Vectors - metabolism Genomics Human bacterial diseases Infectious diseases Macaca Macaca fascicularis Medical sciences Microbiology Miscellaneous Nicotiana - genetics Nicotiana - metabolism Nicotiana benthamiana Pathogens Plague Plague - prevention & control Plague vaccine Plague Vaccine - genetics Plague Vaccine - immunology Plant-produced vaccine Plants - genetics Plants - metabolism Primates Recombinant Proteins - chemistry Tropical bacterial diseases Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Yersinia pestis Yersinia pestis - immunology Yersinia pestis - metabolism Plant-produced vaccine Plague vaccine Yersinia pestis Monkey Vaccine Infection Plague Vertebrata Mammalia Bacteriosis Primates Bacteria Yersiniosis Enterobacteriaceae
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Summary:	Abstract Production of vaccine antigens in plants has received considerable attention over the last decade. However, despite many antigens being expressed in plant systems, and promising efficacy data with rodent models, few vaccine candidates have advanced into studies in non-human primates or human clinical trials. Here, we report on the transient expression of the F1 and LcrV antigens of Yersinia pestis in Nicotiana benthamiana . The antigens were expressed as fusions to the thermostable enzyme of Clostridium thermocellum . When administered to Cynomolgus Macaques the purified plant-produced antigens induced serum IgG and IgA responses specific to F1 and LcrV, and conferred complete protection against lethal challenge with Y. pestis . This study clearly demonstrates the efficacy of a plant-produced plague vaccine candidate in a primate model.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2007.01.017