Activation of transcriptional fusions in Streptomyces lividans resulting from insertion of a 14-bp oligonucleotide

Transcription initiation in the filamentous, antibiotic-producing prokaryote streptomycetes is directed by at least seven different sigma factors that recognize a wide diversity of as yet incompletely characterized promoter sequences. Initial analysis of the DNA signals that initiate transcription a...

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Bibliographic Details
Published in:	Nucleic acids research Vol. 21; no. 17; p. 4151
Main Authors:	Brasch, M.A., Shiffman, D., Cohen, S.N.
Format:	Journal Article
Language:	English
Published:	Oxford Oxford University Press 25-08-1993
Subjects:	Bacterial Proteins - genetics Base Sequence Biological and medical sciences DNA Transposable Elements DNA, Bacterial Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular genetics Molecular Sequence Data Oligodeoxyribonucleotides Recombinant Fusion Proteins - genetics Restriction Mapping Serine Endopeptidases Streptomyces - genetics Streptomyces lividans Transcription. Transcription factor. Splicing. Rna processing Transcriptional Activation Actinomycetales Streptomycetaceae Reporter gene Investigation method Transcription promoter Streptomyces lividans Bacteria Actinomycetes Transcription initiation Hybrid gene
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Summary:	Transcription initiation in the filamentous, antibiotic-producing prokaryote streptomycetes is directed by at least seven different sigma factors that recognize a wide diversity of as yet incompletely characterized promoter sequences. Initial analysis of the DNA signals that initiate transcription and the mechanisms by which these events are regulated typically involves the use of transcriptional fusions in which expression of a reporter gene is taken as indicative of the presence of promoter signals. We report here our finding that a 14-bp oligonucleotide, when placed in the GC-rich context of S.lividans DNA (c.70% G+C), results in strong expression of a reporter gene in S.lividans .
Bibliography:	ark:/67375/HXZ-QRDSGXX5-2 ArticleID:21.17.4151 To whom correspondance should be addressed istex:B56DB921C4DEDE298B51D86645D25BD6EABE44C1 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/21.17.4151