Search Results - "Bras, A. L."

High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery by Turchetto, Jeremy, Sequeira, Ana Filipa, Ramond, Laurie, Peysson, Fanny, Brás, Joana L A, Saez, Natalie J, Duhoo, Yoan, Blémont, Marilyne, Guerreiro, Catarina I P D, Quinton, Loic, De Pauw, Edwin, Gilles, Nicolas, Darbon, Hervé, Fontes, Carlos M G A, Vincentelli, Renaud

“…Animal venoms are complex molecular cocktails containing a wide range of biologically active disulphide-reticulated peptides that target, with high selectivity…”
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Trends, seasonality and forecasts of pulmonary tuberculosis in Portugal by Bras, A. L., Gomes, D., Filipe, P. A., de Sousa, B., Nunes, C.

“…SETTING: Tuberculosis (TB) is a global public health concern. Surveillance programmes present invaluable epidemiological information regarding its temporal…”
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Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins by Sequeira, Ana Filipa, Brás, Joana L A, Guerreiro, Catarina I P D, Vincentelli, Renaud, Fontes, Carlos M G A

“…Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is…”
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A novel α-L-arabinofuranosidase of family 43 glycoside hydrolase (Ct43Araf) from Clostridium thermocellum by Ahmed, Shadab, Luis, Ana Sofia, Bras, Joana L A, Ghosh, Arabinda, Gautam, Saurabh, Gupta, Munishwar N, Fontes, Carlos M G A, Goyal, Arun

“…The study describes a comparative analysis of biochemical, structural and functional properties of two recombinant derivatives from Clostridium thermocellum…”
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Thermostable Recombinant β‑(1→4)-Mannanase from C. thermocellum: Biochemical Characterization and Manno-Oligosaccharides Production by Ghosh, Arabinda, Luís, Ana Sofia, Brás, Joana L. A, Fontes, Carlos M. G. A, Goyal, Arun

“…Functional attributes of a thermostable β-(1→4)-mannanase were investigated from Clostridium thermocellum ATCC 27405. Its sequence comparison the exhibited…”
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Surgical or Endovascular Treatment of MCA Aneurysms: An Agreement Study by Boisseau, W, Darsaut, T E, Fahed, R, Findlay, J M, Bourcier, R, Charbonnier, G, Smajda, S, Ognard, J, Roy, D, Gariel, F, Carlson, A P, Shotar, E, Ciccio, G, Marnat, G, Sporns, P B, Gaberel, T, Jecko, V, Weill, A, Biondi, A, Boulouis, G, Bras, A L, Aldea, S, Passeri, T, Boissonneau, S, Bougaci, N, Gentric, J C, Diestro, J D B, Omar, A T, Al-Jehani, H M, Hage, G El, Volders, D, Kaderali, Z, Tsogkas, I, Magro, E, Holay, Q, Zehr, J, Iancu, D, Raymond, J

“…MCA aneurysms are still commonly clipped surgically despite the recent development of a number of endovascular tools and techniques. We measured clinical…”
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Overexpression, crystallization and preliminary X-ray crystallographic analysis of glucuronoxylan xylanohydrolase (Xyn30A) from Clostridium thermocellum by Verma, Anil Kumar, Goyal, Arun, Freire, Filipe, Bule, Pedro, Venditto, Immacolata, Brás, Joana L. A., Santos, Helena, Cardoso, Vânia, Bonifácio, Cecília, Thompson, Andrew, Romão, Maria João, Prates, José A. M., Ferreira, Luís M. A., Fontes, Carlos M. G. A., Najmudin, Shabir

“…The modular carbohydrate‐active enzyme belonging to glycoside hydrolase family 30 (GH30) from Clostridium thermocellum (CtXynGH30) is a cellulosomal protein…”
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Generation of a library of carbohydrate-active enzymes for plant biomass deconstruction by Cardoso Lopes, Vânia Alexandra da Silva, Bras, Joana L. A., Costa, Ines F., Ferreira, Luis M. A., Gama, Luis, Vincentelli, Renaud, Henrissat, Bernard, Fontes, Carlos

“…Áreas de pesquisa: Biochemistry & Molecular Biology ; Chemistry In nature, the deconstruction of plant carbohydrates is carried out by carbohydrate-active…”
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Structure and Function of an Arabinoxylan-specific Xylanase by Correia, Márcia A.S., Mazumder, Koushik, Brás, Joana L.A., Firbank, Susan J., Zhu, Yanping, Lewis, Richard J., York, William S., Fontes, Carlos M.G.A., Gilbert, Harry J.

“…The enzymatic degradation of plant cell walls plays a central role in the carbon cycle and is of increasing environmental and industrial significance. The…”
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Purification, crystallization and preliminary X-ray characterization of the pentamodular arabinoxylanase CtXyl5A from Clostridium thermocellum by Brás, Joana L. A., Correia, Márcia A. S., Romão, Maria J., Prates, José A. M., Fontes, Carlos M. G. A., Najmudin, Shabir

“…The cellulosome, a highly elaborate extracellular multi‐enzyme complex of cellulases and hemicellulases, is responsible for the degradation of plant cell…”
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Structural insights into a unique cellulase fold and mechanism of cellulose hydrolysis by Brás, Joana L.A, Cartmell, Alan, Carvalho, Ana Luísa M, Verzé, Genny, Bayer, Edward A, Vazana, Yael, Correia, Márcia A.S, Prates, José A.M, Ratnaparkhe, Supriya, Boraston, Alisdair B, Romão, Maria J, Fontes, Carlos M.G.A, Gilbert, Harry J

“…Clostridium thermocellum is a well-characterized cellulose-degrading microorganism. The genome sequence of C. thermocellum encodes a number of proteins that…”
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Deciphering ligand specificity of a Clostridium thermocellum family 35 carbohydrate binding module (CtCBM35) for gluco- and galacto- substituted mannans and its calcium induced stability by Ghosh, Arabinda, Luís, Ana Sofia, Brás, Joana L A, Pathaw, Neeta, Chrungoo, Nikhil K, Fontes, Carlos M G A, Goyal, Arun

“…This study investigated the role of CBM35 from Clostridium thermocellum (CtCBM35) in polysaccharide recognition. CtCBM35 was cloned into pET28a (+) vector with…”
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Mapping Molecular Recognition of β1,3-1,4-Glucans by a Surface Glycan-Binding Protein from the Human Gut Symbiont Bacteroides ovatus by Correia, Viviana G, Trovão, Filipa, Pinheiro, Benedita A, Brás, Joana L A, Silva, Lisete M, Nunes, Cláudia, Coimbra, Manuel A, Liu, Yan, Feizi, Ten, Fontes, Carlos M G A, Mulloy, Barbara, Chai, Wengang, Carvalho, Ana Luísa, Palma, Angelina S

“…A multigene polysaccharide utilization locus (PUL) encoding enzymes and surface carbohydrate (glycan)-binding proteins (SGBPs) was recently identified in…”
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ArsC3 from Desulfovibrio alaskensis G20, a cation and sulfate-independent highly efficient arsenate reductase by Nunes, Catarina I. P., Brás, Joana L. A., Najmudin, Shabir, Moura, José J. G., Moura, Isabel, Carepo, Marta S. P.

“…Desulfovibrio alaskensis G20, a sulfate-reducing bacterium, contains an arsRBC2C3 operon that encodes two putative arsenate reductases, DaG20_ArsC2 and…”
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The Mechanism by Which Arabinoxylanases Can Recognize Highly Decorated Xylans by Labourel, Aurore, Crouch, Lucy I, Brás, Joana L A, Jackson, Adam, Rogowski, Artur, Gray, Joseph, Yadav, Madhav P, Henrissat, Bernard, Fontes, Carlos M G A, Gilbert, Harry J, Najmudin, Shabir, Baslé, Arnaud, Cuskin, Fiona

“…The enzymatic degradation of plant cell walls is an important biological process of increasing environmental and industrial significance. Xylan, a major…”
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Evolution of the feruloyl esterase MtFae1a from Myceliophthora thermophila towards improved catalysts for antioxidants synthesis by Varriale, Simona, Cerullo, Gabriella, Antonopoulou, Io, Christakopoulos, Paul, Rova, Ulrika, Tron, Thierry, Fauré, Régis, Jütten, Peter, Piechot, Alexander, Brás, Joana L. A., Fontes, Carlos M. G. A., Faraco, Vincenza

“…The chemical syntheses currently employed for industrial purposes, including in the manufacture of cosmetics, present limitations such as unwanted side…”
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Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila StCBM64C by Pires, Virgínia M.R., Pereira, Pedro M.M., Brás, Joana L.A., Correia, Márcia, Cardoso, Vânia, Bule, Pedro, Alves, Victor D., Najmudin, Shabir, Venditto, Immacolata, Ferreira, Luís M.A., Romão, Maria João, Carvalho, Ana Luísa, Fontes, Carlos M.G.A., Prazeres, Duarte Miguel

“…Deconstruction of cellulose, the most abundant plant cell wall polysaccharide, requires the cooperative activity of a large repertoire of microbial enzymes…”
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Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization by Dilokpimol, Adiphol, Mäkelä, Miia R., Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L.A., Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P.

“…[Display omitted] •Over 150 putative fungal GEs were identified by genome mining.•Putative fungal GEs were classified into 8 phylogenetic sub-groups.•New…”
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Diverse specificity of cellulosome attachment to the bacterial cell surface by Brás, Joana L. A., Pinheiro, Benedita A., Cameron, Kate, Cuskin, Fiona, Viegas, Aldino, Najmudin, Shabir, Bule, Pedro, Pires, Virginia M. R., Romão, Maria João, Bayer, Edward A., Spencer, Holly L., Smith, Steven, Gilbert, Harry J., Alves, Victor D., Carvalho, Ana Luísa, Fontes, Carlos M. G. A.

“…During the course of evolution, the cellulosome, one of Nature’s most intricate multi-enzyme complexes, has been continuously fine-tuned to efficiently…”
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A Novel Platform for High-Throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli by Sequeira, Ana Filipa, Brás, Joana L A, Fernandes, Vânia O, Guerreiro, Catarina I P D, Vincentelli, Renaud, Fontes, Carlos M G A

“…Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is…”
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