Zebrafish prss59.1 is involved in chorion development

[Display omitted] •A gene knock out (KO) strain of zebrafish prss59.1 gene was established.•The chorion of KO fish did not elevate.•Two proteins, vitellogenin 5 and microfibril-associated glycoprotein 4-like protein, were differentially detected in proteins on chorion.•Fiber-supported knob-like stru...

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Published in:	General and comparative endocrinology Vol. 349; p. 114453
Main Authors:	Rezanujjaman, Md, Pachoensuk, Theeranukul, Forhad Hossain, Md, Maisum Sarwar Jyoti, Md, Rubel Rana, Md, Tsutsumi, Eisei, Mouri, Takumi, Bramastri Susilo, Maria, Wanlada, Klangnurak, Yamamoto, Chihiro, Hasan Ali, Md, Tokumoto, Toshinobu
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-04-2024
Subjects:	Chorion Fertilization Prss59.1 Zebrafish Zebrafish Chorion Fertilization Prss59.1
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Summary:	[Display omitted] •A gene knock out (KO) strain of zebrafish prss59.1 gene was established.•The chorion of KO fish did not elevate.•Two proteins, vitellogenin 5 and microfibril-associated glycoprotein 4-like protein, were differentially detected in proteins on chorion.•Fiber-supported knob-like structures (KS) on the chorion of KO fish showed an abnormal structure.•Prss59.1 is involved in chorion elevation and the formation of KS on the chorion. The prss59.1 gene was identified as one of 11 genes that were highly upregulated during the induction of ovulation in zebrafish by using an in vivo ovulation assay. Previously, we conducted biochemical characterization of Prss59.1 and revealed it to be a trypsin-like proteolytic enzyme. In this study, we established a prss59.1 gene knockout strain using the CRISPR/Cas9 system. Phenotypic analysis of prss59.1 knockout fish showed that prss59.1 is associated with chorion elevation, a prominent event in egg activation during fertilization. The chorions of heterozygous and homozygous prss59.1 mutant zebrafish were smaller than those of the wild type. The results suggested that Prss59.1 is necessary for chorion expansion. The homozygous prss59.1 mutant strain, with a small chorion, showed an extremely low survival rate. Fiber-supported knob-like structures (KS) on the chorion showed an abnormal structure in prss59.1 mutants. Prss59.1 was detected in the KS on the chorion. The pores on the chorion were smaller in the prss59.1 mutants than in the wild type. Transmission electron microscopy (TEM) observations of the cross sections of the chorions showed abnormalities in the chorion structure in prss59.1 mutants. These results demonstrated that Prss59.1 is involved in chorion elevation and in proper formation of the chorion, which is necessary for embryo development.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0016-6480 1095-6840
DOI:	10.1016/j.ygcen.2024.114453