Search Results - "Brakenhoff, G"

Third harmonic generation microscopy by Squier, J, Muller, M, Brakenhoff, G, Wilson, K R

“…Third harmonic generation microscopy is used to make dynamical images of living systems for the first time. A 100 fs excitation pulse at 1.2 aem results in a…”
Get full text

Image calibration in fluorescence microscopy by ZWIER, J. M., VAN ROOIJ, G. J., HOFSTRAAT, J. W., BRAKENHOFF, G. J.

“…Summary A fluorescence image calibration method is presented based on the use of standardized uniformly fluorescing reference layers. It is demonstrated to be…”
Get full text

Dynamics of three-dimensional replication patterns during the S-phase, analysed by double labelling of DNA and confocal microscopy by MANDERS, E. M. M, STAP, J, BRAKENHOFF, G. J, VAN DRIEL, R, ATEN, J. A

“…The temporal and spatial progression of DNA replication in interphase nuclei of eukaryotic cells has been investigated. Application of a recently developed…”
Get full text

Analysis of the influence of spherical aberration from focusing through a dielectric slab in quantitative nonlinear optical susceptibility measurements using third-harmonic generation by Pillai, Rajesh S, Brakenhoff, G J, Müller, M

“…The third-order nonlinear susceptibility (chi(3)) can be measured quantitatively using third-harmonic generation (THG) from two different interfaces. For the…”
Get full text

Imaging colloidal particle induced topological defects in a nematic liquid crystal using third harmonic generation microscopy by Pillai, Rajesh S, Oh-E, Masahito, Yokoyama, Hiroshi, Brakenhoff, G J, Müller, Michiel

“…The nature of the third-harmonic generation (THG) process in a nematic liquid crystal is investigated for the case of tightly focused, low intensity, laser…”
Get full text

Characterization of sectioning fluorescence microscopy with thin uniform fluorescent layers: Sectioned Imaging Property or SIPcharts by BRAKENHOFF, G. J., WURPEL, G. W. H., JALINK, K., OOMEN, L., BROCKS, L., ZWIER, J. M.

“…Summary Thin, uniformly fluorescing reference layers can be used to characterize the imaging conditions in confocal, or more general, sectioning microscopy…”
Get full text

3D microscopy of transparent objects using third‐harmonic generation by Müller, Squier, Wilson, Brakenhoff

“…It is demonstrated that third‐harmonic generation (THG) near interfaces in the refractive index or the third‐order nonlinear susceptibility (χ(3)) permits…”
Get full text

Quantitative image correction and calibration for confocal fluorescence microscopy using thin reference layers and SIPchart-based calibration procedures by ZWIER, J.M, OOMEN, L, BROCKS, L, JALINK, K, BRAKENHOFF, G.J

“…The fluorescence intensity image of an axially integrated through-focus series of a thin standardized uniform fluorescent layer can be used for image intensity…”
Get full text

Immersion oil for high-resolution live-cell imaging at 37 degrees C: optical and physical characteristics by Oomen, L C J M, Sacher, R, Brocks, H H J, Zwier, J M, Brakenhoff, G J, Jalink, K

“…The use of normal immersion oil, developed for 23 degrees C, at 37 degrees C greatly compromises both axial resolution and signal intensity. We developed and…”
Get full text

A new method to study shape recovery of red blood cells using multiple optical trapping by Bronkhorst, P.J., Streekstra, G.J., Grimbergen, J., Nijhof, E.J., Sixma, J.J., Brakenhoff, G.J.

“…In this new method for studying the shape recovery of deformed red blood cells, three optical traps ("optical tweezers") induce a parachute-shaped red cell…”
Get full text

Real-time two-photon confocal microscopy using a femtosecond, amplified Ti:sapphire system by Brakenhoff, G J, Squier, J, Norris, T, Bliton, A C, Wade, M H, Athey, B

“…The bilateral imaging approach known from confocal applications operating in the line mode was used to realize real-time two-photon imaging. It is shown that…”
Get more information

Four-dimensional imaging of chromatin dynamics during the assembly of the interphase nucleus by Manders, E M M, Visser, A E, Koppen, A, de Leeuw, W C, van Liere, R, Brakenhoff, G J, van Driel, R

“…Large-scale chromatin organization is likely to play an important role in epigenetic control of gene expression. This implies that after mitosis the correct…”
Get full text

3-Dimensional super-resolution by spectrally selective imaging by van Oijen, A.M, Köhler, J, Schmidt, J, Müller, M, Brakenhoff, G.J

“…The mutual spatial positions of individual pentacene molecules embedded in a p-terphenyl host crystal, residing within the same diffraction-limited volume, are…”
Get full text

Real time two‐photon absorption microscopy using multi point excitation by Buist, Müller, Squier, Brakenhoff

“…In this communication we present the development of a real time two‐photon absorption microscope, based on parallel excitation with many foci. This pattern of…”
Get full text

The mechanism of red cell (dis)aggregation investigated by means of direct cell manipulation using multiple optical trapping by BRONKHORST, P. J. H., GRIMBERGEN, J., BRAKENHOFF, G. J., HEETHAAR, R. M., SIXMA, J. J.

“…We used multiple optical trapping to study the mechanism of red cell (dis)aggregation. Two sets of optical ‘tweezers’ were used to bring two red blood cells…”
Get full text

Optical far‐field microscopy of single molecules with 3.4 nm lateral resolution by Bloeß, A., Durand, Y., Matsushita, M., Van Dermeer, H., Brakenhoff, G. J., Schmidt, J.

“…Summary Optical far‐field imaging of single molecules in a frozen solution at 1.2 K with a lateral resolution of 3.4 nm is reported. The mechanical stability…”
Get full text

Ki-67 detects a nuclear matrix-associated proliferation-related antigen. I: Intracellular localization during interphase by VERHEIJEN, R, KUIJPERS, H. J. H, SCHLINGEMANN, R. O, BOEHMER, A. L. M, VAN DRIEL, R, BRAKENHOFF, G. J, RAMAEKERS, F. C. S

“…Ki-67 is a commercially available mouse monoclonal antibody, which reacts with a nuclear antigen in proliferating cells. The antibody can be used to determine…”
Get full text

A bioactive designer cytokine for human hematopoietic progenitor cell expansion by Fischer, Martina, Goldschmitt, Jutta, Peschel, Christian, Brakenhoff, Just P. G, Kallen, Karl-Josef, Wollmer, Axel, Grötzinger, Joachim, Rose-John, Stefan

Get full text

Ki-67 detects a nuclear matrix-associated proliferation-related antigen. II: Localization in mitotic cells and association with chromosomes by VERHEIJEN, R, KUIJPERS, H. J. H, VAN DRIEL, R, BECK, J. L. M, VAN DIERENDONCK, J. H, BRAKENHOFF, G. J, RAMAEKERS, F. C. S

“…In interphase cells the proliferation-associated antigen recognized by monoclonal antibody Ki-67 is almost exclusively located in the nucleoli. When cells at…”
Get full text

Analysis of efficiency of two-photon versus single-photon absorption of fluorescence generation in biological objects by Brakenhoff, G J, Müller, M, Ghauharali, R I

“…Starting from basic absorption cross-section values and breakdown limitations on possible field strengths it is shown that the total exposure required for…”
Get more information