Interference by o-phenanthroline in the radioimmunoassay of angiotensin II in small volume blood samples

Interference by o-phenanthroline in the radioimmunoassay of angiotensin II in small volume blood samples

To investigate the ratio of enzyme inhibitor volume to blood volume in angiotensin II (Ang II) blood collection tubes. Whole blood was mixed with known volumes of inhibitor prior to angiotensin II analysis. Imunoreactive Ang II levels increased at low blood volume to high inhibitor volume ratios, du...

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Bibliographic Details
Published in:Clinical biochemistry Vol. 45; no. 1-2; pp. 168 - 170
Main Authors: Braithwaite, Joy E., Yandle, Tim G., Nicholls, M. Gary, Lewis, Lynley K.
Format: Journal Article
Language:English
Published: Amsterdam Elsevier Inc 01-01-2012
Elsevier
Subjects:
Angiotensin II
Angiotensin II - antagonists & inhibitors
Angiotensin II - metabolism
Biological and medical sciences
Blood Pressure - drug effects
Blood Volume - drug effects
Buffers
Drug Therapy, Combination - methods
Enzyme Inhibitors - pharmacology
Humans
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Models, Statistical
o-Phenanthroline
Pediatric blood sampling
Phenanthrolines - pharmacology
Protease Inhibitors - pharmacology
Radioimmunoassay
Radioimmunoassay - methods
o-phenanthroline
ACE
Radioimmunoassay
Ir
Pediatric blood sampling
Angiotensin II
Ang II
HPLC
Human
Pediatrics
Peptide hormone
Interference
Samplings
o-Phenanthroline
Blood
Octapeptide
Renin angiotensin system
Blood volume
Clinical biology
Small sample
Sampling
Child
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Summary:To investigate the ratio of enzyme inhibitor volume to blood volume in angiotensin II (Ang II) blood collection tubes. Whole blood was mixed with known volumes of inhibitor prior to angiotensin II analysis. Imunoreactive Ang II levels increased at low blood volume to high inhibitor volume ratios, due to interference by o-phenanthroline. To prevent falsely elevated Ang II levels in low volume blood samples an appropriate volume of inhibitor solution must be used. ► We assessed enzyme inhibitor volume to blood volume in angiotensin II measurement. ► Blood was mixed with known volumes of inhibitor prior to angiotensin II analysis. ► Angiotensin II levels increased at low blood volume to high inhibitor volume ratio. ► o-Phenanthroline increases immunoreactive angiotensin II levels. ► Match inhibitor volume to blood volume when measuring angiotensin II in plasma.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2011.10.008