Genome sequence of the pattern forming Paenibacillus vortex bacterium reveals potential for thriving in complex environments

The pattern-forming bacterium Paenibacillus vortex is notable for its advanced social behavior, which is reflected in development of colonies with highly intricate architectures. Prior to this study, only two other Paenibacillus species (Paenibacillus sp. JDR-2 and Paenibacillus larvae) have been se...

Full description

Saved in:

Bibliographic Details
Published in:	BMC genomics Vol. 11; no. 1; p. 710
Main Authors:	Sirota-Madi, A, Olender, T, Helman, Y, Ingham, C, Brainis, I, Roth, D, Hagi, E, Brodsky, L, Leshkowitz, D, Galatenko, V, Nikolaev, V, Mugasimangalam, R.C, Bransburg-Zabary, S, Gutnick, D.L, Lancet, D, Ben-Jacob, E
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 17-12-2010 BioMed Central BMC
Subjects:	bacillus-subtilis Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Pairing - genetics Base Sequence Chemotaxis - genetics colonial development Colony Count, Microbial Environment Flagella - genetics Flagella - ultrastructure Food Genes Genes, Bacterial - genetics Genetic aspects Genome, Bacterial - genetics Genomes Genomics iv pili Microorganisms Microscopy Motility multicellular organism Multienzyme Complexes - genetics Multigene Family myxococcus-xanthus neighbor-joining method Nucleotide sequence Oligonucleotide Array Sequence Analysis Paenibacillus - cytology Paenibacillus - genetics Paenibacillus - growth & development Paenibacillus - ultrastructure Phylogeny Physiological aspects protein families database Repetitive Sequences, Nucleic Acid - genetics Reproducibility of Results self-organization Sequence Analysis, DNA signaling pathways Standard deviation swarming motility United States Netherlands Israel
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The pattern-forming bacterium Paenibacillus vortex is notable for its advanced social behavior, which is reflected in development of colonies with highly intricate architectures. Prior to this study, only two other Paenibacillus species (Paenibacillus sp. JDR-2 and Paenibacillus larvae) have been sequenced. However, no genomic data is available on the Paenibacillus species with pattern-forming and complex social motility. Here we report the de novo genome sequence of this Gram-positive, soil-dwelling, sporulating bacterium. The complete P. vortex genome was sequenced by a hybrid approach using 454 Life Sciences and Illumina, achieving a total of 289× coverage, with 99.8% sequence identity between the two methods. The sequencing results were validated using a custom designed Agilent microarray expression chip which represented the coding and the non-coding regions. Analysis of the P. vortex genome revealed 6,437 open reading frames (ORFs) and 73 non-coding RNA genes. Comparative genomic analysis with 500 complete bacterial genomes revealed exceptionally high number of two-component system (TCS) genes, transcription factors (TFs), transport and defense related genes. Additionally, we have identified genes involved in the production of antimicrobial compounds and extracellular degrading enzymes. These findings suggest that P. vortex has advanced faculties to perceive and react to a wide range of signaling molecules and environmental conditions, which could be associated with its ability to reconfigure and replicate complex colony architectures. Additionally, P. vortex is likely to serve as a rich source of genes important for agricultural, medical and industrial applications and it has the potential to advance the study of social microbiology within Gram-positive bacteria.
Bibliography:	201107488 E50 http://edepot.wur.nl/175051 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1471-2164 1471-2164
DOI:	10.1186/1471-2164-11-710