Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors

Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors

In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the m...

Full description

Saved in:
Bibliographic Details
Published in:Biocell Vol. 21; no. 1; p. 39
Main Authors: Vanzulli, S, Gazzaniga, S, Braidot, M F, Vecchi, A, Mantovani, A, Wainstok de Calmanovici, R
Format: Journal Article
Language:English
Published: Argentina 01-04-1997
Subjects:
Adenocarcinoma
Animals
Antibodies, Monoclonal
Antibody Specificity
Antigen-Antibody Reactions
Endothelium, Vascular - chemistry
Endothelium, Vascular - cytology
Endothelium, Vascular - immunology
Female
Immunohistochemistry
Mammary Glands, Animal - blood supply
Mammary Glands, Animal - cytology
Mammary Neoplasms, Experimental
Mice
Mice, Inbred BALB C
Microcirculation
Tumor Cells, Cultured
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the murine form of CD31/PECAM. This mAb with immunoenzymatic technique or immunofluorescent labelling, was found to be a useful tool to quantify tumor neovascularization. Specifically, membrane reinforcement could be observed in vessel ECs, indicating the expression of CD31/ PECAM in their surface. The staining of ECs from tumors and from normal tissues was also compared. In this work, the use of MEC13.3 mAb is reported to recognize mice mammary tumor ECs as a useful tool to identify neovascularization. It would also be helpful for research on the origin and function of vascular endothelium in murine tumor experimental models.
ISSN:0327-9545