Observational study on variability between biobanks in the estimation of DNA concentration

There is little confidence in the consistency of estimation of DNA concentrations when samples move between laboratories. Evidence on this consistency is largely anecdotal. Therefore there is a need first to measure this consistency among different laboratories and then identify and implement remedi...

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Bibliographic Details
Published in:	BMC research notes Vol. 2; no. 1; p. 208
Main Authors:	Brown, Jay, Donev, Alexander N, Aslanidis, Charalampos, Bracegirdle, Pippa, Dixon, Katherine P, Foedinger, Manuela, Gwilliam, Rhian, Hardy, Matthew, Illig, Thomas, Ke, Xiayi, Krinka, Dagni, Lagerberg, Camilla, Laiho, Päivi, Lewis, David H, McArdle, Wendy, Patton, Simon, Ring, Susan M, Schmitz, Gerd, Stevens, Helen, Tybring, Gunnel, Wichmann, H Erich, Ollier, William E R, Yuille, Martin A
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 13-10-2009 BioMed Central BMC
Subjects:	Administrative support Colleges & universities DNA Epidemiology Fluorescence spectroscopy Genetic testing Genomes Laboratories Measurement Shipments Short Report Statistical analysis Statistical methods United Kingdom Finland
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Summary:	There is little confidence in the consistency of estimation of DNA concentrations when samples move between laboratories. Evidence on this consistency is largely anecdotal. Therefore there is a need first to measure this consistency among different laboratories and then identify and implement remedies. A pilot experiment to test logistics and provide initial data on consistency was therefore conceived. DNA aliquots at nominal concentrations between 10 and 300 ng/mul were dispensed into the wells of 96-well plates by one participant - the coordinating centre. Participants estimated the concentration in each well and returned estimates to the coordinating centre. Considerable overall variability was observed among estimates. There were statistically significant differences between participants' measurements and between fluorescence emission and absorption spectroscopy. Anecdotal evidence of variability in DNA concentration estimation has been substantiated. Reduction in variability between participants will require the identification of major sources of variation, specification of effective remedies and their implementation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1756-0500 1756-0500
DOI:	10.1186/1756-0500-2-208