Search Results - "Brás, Joana L. A"

Thermostable Recombinant β‑(1→4)-Mannanase from C. thermocellum: Biochemical Characterization and Manno-Oligosaccharides Production by Ghosh, Arabinda, Luís, Ana Sofia, Brás, Joana L. A, Fontes, Carlos M. G. A, Goyal, Arun

“…Functional attributes of a thermostable β-(1→4)-mannanase were investigated from Clostridium thermocellum ATCC 27405. Its sequence comparison the exhibited…”
Get full text

Generation of a library of carbohydrate-active enzymes for plant biomass deconstruction by Cardoso Lopes, Vânia Alexandra da Silva, Bras, Joana L. A., Costa, Ines F., Ferreira, Luis M. A., Gama, Luis, Vincentelli, Renaud, Henrissat, Bernard, Fontes, Carlos

“…Áreas de pesquisa: Biochemistry & Molecular Biology ; Chemistry In nature, the deconstruction of plant carbohydrates is carried out by carbohydrate-active…”
Get full text

Structural insights into a unique cellulase fold and mechanism of cellulose hydrolysis by Brás, Joana L.A, Cartmell, Alan, Carvalho, Ana Luísa M, Verzé, Genny, Bayer, Edward A, Vazana, Yael, Correia, Márcia A.S, Prates, José A.M, Ratnaparkhe, Supriya, Boraston, Alisdair B, Romão, Maria J, Fontes, Carlos M.G.A, Gilbert, Harry J

“…Clostridium thermocellum is a well-characterized cellulose-degrading microorganism. The genome sequence of C. thermocellum encodes a number of proteins that…”
Get full text

Deciphering ligand specificity of a Clostridium thermocellum family 35 carbohydrate binding module (CtCBM35) for gluco- and galacto- substituted mannans and its calcium induced stability by Ghosh, Arabinda, Luís, Ana Sofia, Brás, Joana L A, Pathaw, Neeta, Chrungoo, Nikhil K, Fontes, Carlos M G A, Goyal, Arun

“…This study investigated the role of CBM35 from Clostridium thermocellum (CtCBM35) in polysaccharide recognition. CtCBM35 was cloned into pET28a (+) vector with…”
Get full text

Mapping Molecular Recognition of β1,3-1,4-Glucans by a Surface Glycan-Binding Protein from the Human Gut Symbiont Bacteroides ovatus by Correia, Viviana G, Trovão, Filipa, Pinheiro, Benedita A, Brás, Joana L A, Silva, Lisete M, Nunes, Cláudia, Coimbra, Manuel A, Liu, Yan, Feizi, Ten, Fontes, Carlos M G A, Mulloy, Barbara, Chai, Wengang, Carvalho, Ana Luísa, Palma, Angelina S

“…A multigene polysaccharide utilization locus (PUL) encoding enzymes and surface carbohydrate (glycan)-binding proteins (SGBPs) was recently identified in…”
Get full text

ArsC3 from Desulfovibrio alaskensis G20, a cation and sulfate-independent highly efficient arsenate reductase by Nunes, Catarina I. P., Brás, Joana L. A., Najmudin, Shabir, Moura, José J. G., Moura, Isabel, Carepo, Marta S. P.

“…Desulfovibrio alaskensis G20, a sulfate-reducing bacterium, contains an arsRBC2C3 operon that encodes two putative arsenate reductases, DaG20_ArsC2 and…”
Get full text

The Mechanism by Which Arabinoxylanases Can Recognize Highly Decorated Xylans by Labourel, Aurore, Crouch, Lucy I, Brás, Joana L A, Jackson, Adam, Rogowski, Artur, Gray, Joseph, Yadav, Madhav P, Henrissat, Bernard, Fontes, Carlos M G A, Gilbert, Harry J, Najmudin, Shabir, Baslé, Arnaud, Cuskin, Fiona

“…The enzymatic degradation of plant cell walls is an important biological process of increasing environmental and industrial significance. Xylan, a major…”
Get full text

Evolution of the feruloyl esterase MtFae1a from Myceliophthora thermophila towards improved catalysts for antioxidants synthesis by Varriale, Simona, Cerullo, Gabriella, Antonopoulou, Io, Christakopoulos, Paul, Rova, Ulrika, Tron, Thierry, Fauré, Régis, Jütten, Peter, Piechot, Alexander, Brás, Joana L. A., Fontes, Carlos M. G. A., Faraco, Vincenza

“…The chemical syntheses currently employed for industrial purposes, including in the manufacture of cosmetics, present limitations such as unwanted side…”
Get full text

Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila StCBM64C by Pires, Virgínia M.R., Pereira, Pedro M.M., Brás, Joana L.A., Correia, Márcia, Cardoso, Vânia, Bule, Pedro, Alves, Victor D., Najmudin, Shabir, Venditto, Immacolata, Ferreira, Luís M.A., Romão, Maria João, Carvalho, Ana Luísa, Fontes, Carlos M.G.A., Prazeres, Duarte Miguel

“…Deconstruction of cellulose, the most abundant plant cell wall polysaccharide, requires the cooperative activity of a large repertoire of microbial enzymes…”
Get full text

Structure and Function of an Arabinoxylan-specific Xylanase by Correia, Márcia A.S., Mazumder, Koushik, Brás, Joana L.A., Firbank, Susan J., Zhu, Yanping, Lewis, Richard J., York, William S., Fontes, Carlos M.G.A., Gilbert, Harry J.

“…The enzymatic degradation of plant cell walls plays a central role in the carbon cycle and is of increasing environmental and industrial significance. The…”
Get full text

Fungal glucuronoyl esterases: Genome mining based enzyme discovery and biochemical characterization by Dilokpimol, Adiphol, Mäkelä, Miia R., Cerullo, Gabriella, Zhou, Miaomiao, Varriale, Simona, Gidijala, Loknath, Brás, Joana L.A., Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Faraco, Vincenza, Hilden, Kristiina S., de Vries, Ronald P.

“…[Display omitted] •Over 150 putative fungal GEs were identified by genome mining.•Putative fungal GEs were classified into 8 phylogenetic sub-groups.•New…”
Get full text

Diverse specificity of cellulosome attachment to the bacterial cell surface by Brás, Joana L. A., Pinheiro, Benedita A., Cameron, Kate, Cuskin, Fiona, Viegas, Aldino, Najmudin, Shabir, Bule, Pedro, Pires, Virginia M. R., Romão, Maria João, Bayer, Edward A., Spencer, Holly L., Smith, Steven, Gilbert, Harry J., Alves, Victor D., Carvalho, Ana Luísa, Fontes, Carlos M. G. A.

“…During the course of evolution, the cellulosome, one of Nature’s most intricate multi-enzyme complexes, has been continuously fine-tuned to efficiently…”
Get full text

A Novel Platform for High-Throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli by Sequeira, Ana Filipa, Brás, Joana L A, Fernandes, Vânia O, Guerreiro, Catarina I P D, Vincentelli, Renaud, Fontes, Carlos M G A

“…Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is…”
Get more information

Novel Clostridium thermocellum Type I Cohesin-Dockerin Complexes Reveal a Single Binding Mode by Brás, Joana L.A., Alves, Victor D., Carvalho, Ana Luísa, Najmudin, Shabir, Prates, José A.M., Ferreira, Luís M.A., Bolam, David N., Romão, Maria João, Gilbert, Harry J., Fontes, Carlos M.G.A.

“…Protein-protein interactions play a pivotal role in a large number of biological processes exemplified by the assembly of the cellulosome. Integration of…”
Get full text

Directed evolution of the type C feruloyl esterase from Fusarium oxysporum FoFaeC and molecular docking analysis of its improved variants by Cerullo, Gabriella, Varriale, Simona, Bozonnet, Sophie, Antonopoulou, Io, Christakopoulos, Paul, Rova, Ulrika, Gherbovet, Olga, Fauré, Régis, Piechot, Alexander, Jütten, Peter, Brás, Joana L.A., Fontes, Carlos M.G.A., Faraco, Vincenza

“…[Display omitted] •A library of around 30,000 random mutants of FoFaeC was generated.•Screening for enzymatic activity led to the selection of 96 improved…”
Get full text

High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery by Turchetto, Jeremy, Sequeira, Ana Filipa, Ramond, Laurie, Peysson, Fanny, Brás, Joana L A, Saez, Natalie J, Duhoo, Yoan, Blémont, Marilyne, Guerreiro, Catarina I P D, Quinton, Loic, De Pauw, Edwin, Gilles, Nicolas, Darbon, Hervé, Fontes, Carlos M G A, Vincentelli, Renaud

“…Animal venoms are complex molecular cocktails containing a wide range of biologically active disulphide-reticulated peptides that target, with high selectivity…”
Get full text

Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins by Sequeira, Ana Filipa, Brás, Joana L A, Guerreiro, Catarina I P D, Vincentelli, Renaud, Fontes, Carlos M G A

“…Gene synthesis is becoming an important tool in many fields of recombinant DNA technology, including recombinant protein production. De novo gene synthesis is…”
Get full text

Immobilization of bacterial feruloyl esterase on mesoporous silica particles and enhancement of synthetic activity by hydrophobic-modified surface by Chong, Sun Li, Cardoso, Vânia, Brás, Joana L.A., Gomes, Milene Zezzi do Valle, Fontes, Carlos M.G.A., Olsson, Lisbeth

“…[Display omitted] •Screening synthetic ability of bacterial feruloyl esterases immobilized on SBA-15.•The immobilized BFae2 from Butyrivibrio sp. showed the…”
Get full text

High-Throughput Production of a New Library of Human Single and Tandem PDZ Domains Allows Quantitative PDZ-Peptide Interaction Screening Through High-Throughput Holdup Assay by Duhoo, Yoan, Girault, Virginie, Turchetto, Jeremy, Ramond, Laurie, Durbesson, Fabien, Fourquet, Patrick, Nominé, Yves, Cardoso, Vânia, Sequeira, Ana Filipa, Brás, Joana L A, Fontes, Carlos M G A, Travé, Gilles, Wolff, Nicolas, Vincentelli, Renaud

“…PDZ domains recognize PDZ Binding Motifs (PBMs) at the extreme C-terminus of their partner proteins. The human proteome contains 266 identified PDZ domains,…”
Get more information

Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies by Dilokpimol, Adiphol, Mäkelä, Miia R., Varriale, Simona, Zhou, Miaomiao, Cerullo, Gabriella, Gidijala, Loknath, Hinkka, Harri, Brás, Joana L.A., Jütten, Peter, Piechot, Alexander, Verhaert, Raymond, Hildén, Kristiina S., Faraco, Vincenza, de Vries, Ronald P.

“…[Display omitted] •New ascomycete and basidiomycete candidate FAEs were produced in Pichia pastoris.•Most of the candidate FAEs showed comparable activity to…”
Get full text