Evaluation of Alum-Naltrexone Adjuvant Activity, on Efficacy of Anti-Leishmania Immunization with Autoclaved Leishmania major (MRHO/IR/75/ER) Antigens in BALB/C Mice

Evaluation of Alum-Naltrexone Adjuvant Activity, on Efficacy of Anti-Leishmania Immunization with Autoclaved Leishmania major (MRHO/IR/75/ER) Antigens in BALB/C Mice

Naltrexone, an opioid receptor antagonist shifts the immune response toward a Th1 profile. In the current study, we evaluated the efficacy of the mixture of NTX and alum, as a new adjuvant, to enhance immune response and induce protection against Leishmania major in a mouse model. BALB/c mice were i...

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Bibliographic Details
Published in:Iranian journal of parasitology Vol. 9; no. 3; pp. 311 - 318
Main Authors: Mohammadzadeh Hajipirloo, Habib, Bozorgomd, Arezoo, Shahabi, Shahram, Hazrati Tappeh, Khosrow, Karamati, Seyed Ahmad
Format: Journal Article
Language:English
Published: Iran Tehran University of Medical Sciences 01-09-2014
Subjects:
Adjuvant
Alum
Antigen
BALB/c
Immune system
Leishmania major
Naltrexone
Original
Parasites
Parasitic diseases
Vaccines
Iran
Antigen
Adjuvant
Alum
Naltrexone
BALB/c
Leishmania major
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Summary:Naltrexone, an opioid receptor antagonist shifts the immune response toward a Th1 profile. In the current study, we evaluated the efficacy of the mixture of NTX and alum, as a new adjuvant, to enhance immune response and induce protection against Leishmania major in a mouse model. BALB/c mice were immunized three times either autoclaved L. major promastigotes' antigens alone or in combination with the adjuvant alum, naltrexone or the alum-naltrexone mixture. Both humoral and cellular immune responses were assessed two weeks after the last immunization and compared with control mice. The administration of alum- NTX in combination with the parasite antigen, significantly increased production of IFN-γ IFN-γ /IL-5 ratio, lymphocyte proliferation and improved DTH response against L. major. There was no significant difference in survival following challenge among groups. Immunization with the alum- naltrexone mixture as an adjuvant, in combination with the autoclaved L. major promastigotes antigens, can enhance cellular immunity and shift the immune responses to a Th1 pattern.
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ISSN:1735-7020
2008-238X