Production of a cytotoxic human monoclonal antibody with specificity for HLA-DR4 and -DRw10 by cells derived from a highly sensitized kidney recipient

A human monoclonal antibody which reacts preferentially with HLA-DR4 and -DRw10 B-cell targets has been produced. A human B-cell line, secreting antibody which reacted preferentially with DR4 and DR1 targets, was derived from a highly sensitized kidney recipient who had rejected two grafts. This lin...

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Bibliographic Details
Published in:	Human immunology Vol. 27; no. 3; p. 167
Main Authors:	Yendle, J E, Bowerman, P D, Yousaf, K, Roberts, I M, Cohen, B, Barber, L, Lechler, R, Hancock, R J, Bradley, B A
Format:	Journal Article
Language:	English
Published:	United States 01-03-1990
Subjects:	Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antibody Specificity - immunology B-Lymphocytes - immunology Blotting, Western Cell Line Cell Separation Cytotoxicity, Immunologic - immunology Flow Cytometry HLA-DR Antigens - immunology HLA-DR4 Antigen - immunology Humans Hybridomas - metabolism Kidney Transplantation - immunology Mice Polymorphism, Restriction Fragment Length Transfection
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Summary:	A human monoclonal antibody which reacts preferentially with HLA-DR4 and -DRw10 B-cell targets has been produced. A human B-cell line, secreting antibody which reacted preferentially with DR4 and DR1 targets, was derived from a highly sensitized kidney recipient who had rejected two grafts. This line was fused with the mouse myeloma P3X63Ag8.653 and a selected hybridoma cloned. The clones secrete IgM(lambda), which reacts strongly with HLA-DR4 and -DRw10 and more weakly with -DRw14 and a proportion of -DR1 B cells in cytotoxicity assays. Using B-cell lines as targets in cytotoxicity and enzyme-linked immunosorbent assays, the antibody gives a broader pattern of reaction, reacting with HLA-DR1, -DR4, -DR9, -DRw10, -DRw14, and some -DR2 targets. The antibody (NI) is currently in use as a reagent for tissue typing.
ISSN:	0198-8859
DOI:	10.1016/0198-8859(90)90048-T