Search Results - "Bouwknegt, Jonna"
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Identification of Oxygen-Independent Pathways for Pyridine Nucleotide and Coenzyme A Synthesis in Anaerobic Fungi by Expression of Candidate Genes in Yeast
Published in mBio (29-06-2021)“…Neocallimastigomycetes are unique examples of strictly anaerobic eukaryotes. This study investigates how these anaerobic fungi bypass reactions involved in…”
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Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis
Published in Fungal biology and biotechnology (16-10-2021)“…Abstract Background In most fungi, quinone-dependent Class-II dihydroorotate dehydrogenases (DHODs) are essential for pyrimidine biosynthesis. Coupling of…”
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A squalene–hopene cyclase in Schizosaccharomyces japonicus represents a eukaryotic adaptation to sterol-limited anaerobic environments
Published in Proceedings of the National Academy of Sciences - PNAS (10-08-2021)“…Biosynthesis of sterols, which are key constituents of canonical eukaryotic membranes, requiresmolecular oxygen. Anaerobic protists and deep-branching…”
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Anaerobic growth of Saccharomyces cerevisiae CEN.PK113-7D does not depend on synthesis or supplementation of unsaturated fatty acids
Published in FEMS yeast research (01-09-2019)“…ABSTRACT In Saccharomyces cerevisiae, acyl-coenzyme A desaturation by Ole1 requires molecular oxygen. Tween 80, a poly-ethoxylated sorbitan-oleate ester, is…”
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Critical parameters and procedures for anaerobic cultivation of yeasts in bioreactors and anaerobic chambers
Published in FEMS yeast research (01-08-2021)“…ABSTRACT All known facultatively fermentative yeasts require molecular oxygen for growth. Only in a small number of yeast species, these requirements can be…”
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Identification of fungal dihydrouracil-oxidase genes by expression in Saccharomyces cerevisiae
Published in Antonie van Leeuwenhoek (01-11-2022)“…Analysis of predicted fungal proteomes revealed a large family of sequences that showed similarity to the Saccharomyces cerevisiae Class-I dihydroorotate…”
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Laboratory evolution of a glucose-phosphorylation-deficient, arabinose-fermenting S. cerevisiae strain reveals mutations in GAL2 that enable glucose-insensitive l-arabinose uptake
Published in FEMS yeast research (01-09-2018)“…Cas9-assisted genome editing was used to construct an engineered glucose-phosphorylation-negative S. cerevisiae strain, expressing the Lactobacillus…”
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