Is the Simple Saline Mount Technique More Effective than Potassium Hydroxide for the Microscopic Detection of Sarcoptes scabiei?
Even though various assays have been developed such as epiluminescence microscopy, enzymelinked immunosorbent assay, and real-time quantitative polymerase chain reaction (Wong et al., 2015), the microscopic examination of skin scrapings remains the gold standard for diagnosis as well as the least ex...
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| Published in: | The Journal of parasitology Vol. 104; no. 1; p. 109 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
American Society of Parasitologists
01-02-2018
Allen Press Inc |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Even though various assays have been developed such as epiluminescence microscopy, enzymelinked immunosorbent assay, and real-time quantitative polymerase chain reaction (Wong et al., 2015), the microscopic examination of skin scrapings remains the gold standard for diagnosis as well as the least expensive choice. Initially, skin scrapings of the patient were examined with a KOH mount where no mites were observed under both X10 and X40 objectives. Because of strong clinical suspicion, however, a subsequent saline mount was prepared and observed thoroughly. Negative results for mites in skin scrapings are very common in everyday practice and should not exclude the diagnosis of scabies. [...] the dermatologist's approach including the precise communication of a strong clinical suspicion to the laboratory may be decisive. |
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| Bibliography: | ObjectType-Case Study-2 SourceType-Scholarly Journals-1 ObjectType-Feature-4 content type line 23 ObjectType-Report-1 ObjectType-Article-3 |
| ISSN: | 0022-3395 1937-2345 |
| DOI: | 10.1645/17-157 |