Search Results - "Botchan, Michael R."

Mechanisms for initiating cellular DNA replication by Bleichert, Franziska, Botchan, Michael R., Berger, James M.

“…Cellular DNA replication factories depend on ring-shaped hexameric helicases to aid DNA synthesis by processively unzipping the parental DNA helix. Replicative…”
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Mechanisms and regulation of DNA replication initiation in eukaryotes by Parker, Matthew W, Botchan, Michael R, Berger, James M

“…Cellular DNA replication is initiated through the action of multiprotein complexes that recognize replication start sites in the chromosome (termed origins)…”
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Crystal structure of the eukaryotic origin recognition complex by Bleichert, Franziska, Botchan, Michael R., Berger, James M.

“…Initiation of cellular DNA replication is tightly controlled to sustain genomic integrity. In eukaryotes, the heterohexameric origin recognition complex (ORC)…”
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Zelda binding in the early Drosophila melanogaster embryo marks regions subsequently activated at the maternal-to-zygotic transition by Harrison, Melissa M, Li, Xiao-Yong, Kaplan, Tommy, Botchan, Michael R, Eisen, Michael B

“…The earliest stages of development in most metazoans are driven by maternally deposited proteins and mRNAs, with widespread transcriptional activation of the…”
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Stalled Fork Rescue via Dormant Replication Origins in Unchallenged S Phase Promotes Proper Chromosome Segregation and Tumor Suppression by Kawabata, Tsuyoshi, Luebben, Spencer W., Yamaguchi, Satoru, Ilves, Ivar, Matise, Ilze, Buske, Tavanna, Botchan, Michael R., Shima, Naoko

“…Eukaryotic cells license far more origins than are actually used for DNA replication, thereby generating a large number of dormant origins. Accumulating…”
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The structural basis for MCM2-7 helicase activation by GINS and Cdc45 by Botchan, Michael R, Berger, James M, Costa, Alessandro, Ilves, Ivar, Tamberg, Nele, Petojevic, Tatjana, Nogales, Eva

“…In eukaryotes, DNA replication depends on loading the ring-shaped helicase, Mcm2–7 onto double-stranded DNA and subsequent activation by GINS–Cdc45. Using…”
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A new class of disordered elements controls DNA replication through initiator self-assembly by Parker, Matthew W, Bell, Maren, Mir, Mustafa, Kao, Jonchee A, Darzacq, Xavier, Botchan, Michael R, Berger, James M

“…The initiation of DNA replication in metazoans occurs at thousands of chromosomal sites known as origins. At each origin, the Origin Recognition Complex (ORC),…”
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Isolation of the Cdc45/Mcm2-7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase by Moyer, S.E, Lewis, P.W, Botchan, M.R

“…The protein Cdc45 plays a critical but poorly understood role in the initiation and elongation stages of eukaryotic DNA replication. To study Cdc45's function…”
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Molecular Basis for ATP-Hydrolysis-Driven DNA Translocation by the CMG Helicase of the Eukaryotic Replisome by Eickhoff, Patrik, Kose, Hazal B., Martino, Fabrizio, Petojevic, Tatjana, Abid Ali, Ferdos, Locke, Julia, Tamberg, Nele, Nans, Andrea, Berger, James M., Botchan, Michael R., Yardimci, Hasan, Costa, Alessandro

“…In the eukaryotic replisome, DNA unwinding by the Cdc45-MCM-Go-Ichi-Ni-San (GINS) (CMG) helicase requires a hexameric ring-shaped ATPase named minichromosome…”
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Conformational control and DNA-binding mechanism of the metazoan origin recognition complex by Bleichert, Franziska, Leitner, Alexander, Aebersold, Ruedi, Botchan, Michael R., Berger, James M.

“…In eukaryotes, the heterohexameric origin recognition complex (ORC) coordinates replication onset by facilitating the recruitment and loading of the…”
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Cdc45 (cell division cycle protein 45) guards the gate of the Eukaryote Replisome helicase stabilizing leading strand engagement by Petojevic, Tatjana, Pesavento, James J., Costa, Alessandro, Liang, Jingdan, Wang, Zhijun, Berger, James M., Botchan, Michael R.

“…DNA replication licensing is now understood to be the pathway that leads to the assembly of double hexamers of minichromosome maintenance (Mcm2–7) at origin…”
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ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote by Lyubimov, Artem Y, Costa, Alessandro, Bleichert, Franziska, Botchan, Michael R, Berger, James M

“…The heterohexameric minichromosome maintenance (MCM2–7) complex is an ATPase that serves as the central replicative helicase in eukaryotes. During initiation,…”
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Molecular determinants of phase separation for Drosophila DNA replication licensing factors by Parker, Matthew W, Kao, Jonchee A, Huang, Alvin, Berger, James M, Botchan, Michael R

“…Liquid-liquid phase separation (LLPS) of intrinsically disordered regions (IDRs) in proteins can drive the formation of membraneless compartments in cells…”
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Grainyhead and Zelda compete for binding to the promoters of the earliest-expressed Drosophila genes by Harrison, Melissa M., Botchan, Michael R., Cline, Thomas W.

“…Maternally contributed mRNAs and proteins control the initial stages of development following fertilization. During this time, most of the zygotic genome…”
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DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome by Costa, Alessandro, Renault, Ludovic, Swuec, Paolo, Petojevic, Tatjana, Pesavento, James J, Ilves, Ivar, MacLellan-Gibson, Kirsty, Fleck, Roland A, Botchan, Michael R, Berger, James M

“…The Cdc45/Mcm2-7/GINS (CMG) helicase separates DNA strands during replication in eukaryotes. How the CMG is assembled and engages DNA substrates remains…”
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Checkpoint kinase 2 (Chk2) inhibits the activity of the Cdc45/MCM2-7/GINS (CMG) replicative helicase complex by Ilves, Ivar, Tamberg, Nele, Botchan, Michael R

“…The replication of eukaryote chromosomes slows down when DNA is damaged and the proteins that work at the fork (the replisome) are known targets for the…”
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Chromatin reader L(3)mbt requires the Myb–MuvB/DREAM transcriptional regulatory complex for chromosomal recruitment by Blanchard, Daniel P, Georlette, Daphne, Antoszewski, Lisa, Botchan, Michael R

“…Lethal malignant brain tumors (lmbt) result from the loss of the conserved transcriptional repressor l(3)mbt, in Drosophila melanogaster. Similar mutations in…”
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Genomic profiling and expression studies reveal both positive and negative activities for the Drosophila Myb MuvB/dREAM complex in proliferating cells by Georlette, Daphne, Ahn, Soyeon, MacAlpine, David M, Cheung, Evelyn, Lewis, Peter W, Beall, Eileen L, Bell, Stephen P, Speed, Terry, Manak, J Robert, Botchan, Michael R

“…Myb-MuvB (MMB)/dREAM is a nine-subunit complex first described in Drosophila as a repressor of transcription, dependent on E2F2 and the RBFs. Myb, an integral…”
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A Meier-Gorlin syndrome mutation in a conserved C-terminal helix of Orc6 impedes origin recognition complex formation by Bleichert, Franziska, Balasov, Maxim, Chesnokov, Igor, Nogales, Eva, Botchan, Michael R, Berger, James M

“…In eukaryotes, DNA replication requires the origin recognition complex (ORC), a six-subunit assembly that promotes replisome formation on chromosomal origins…”
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Activation of the MCM2-7 Helicase by Association with Cdc45 and GINS Proteins by Ilves, Ivar, Petojevic, Tatjana, Pesavento, James J., Botchan, Michael R.

“…MCM2-7 proteins provide essential helicase functions in eukaryotes at chromosomal DNA replication forks. During the G1 phase of the cell cycle, they remain…”
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