Assessment of Aspergillus fumigatus burden in lungs of intratracheally-challenged turkeys ( Meleagris gallopavo ) by quantitative PCR, galactomannan enzyme immunoassay, and quantitative culture

Abstract Aspergillus fumigatus remains a major respiratory pathogen in birds and treatment is still difficult. We challenged different groups of few-day-old turkeys via intratracheal aerosolisation with increasing concentrations (105 up to 108 ) of conidia using a MicroSprayer® device. The fungal bu...

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Bibliographic Details
Published in:	Comparative immunology, microbiology and infectious diseases Vol. 37; no. 5; pp. 271 - 279
Main Authors:	Melloul, Elise, Thierry, Simon, Durand, Benoit, Cordonnier, Nathalie, Desoubeaux, Guillaume, Chandenier, Jacques, Bostvironnois, Christophe, Botterel, Françoise, Chermette, René, Guillot, Jacques, Arné, Pascal
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-12-2014 Elsevier
Subjects:	Aerosols Allergy and Immunology Animals Aspergillosis Aspergillosis - diagnosis Aspergillosis - microbiology Aspergillosis - mortality Aspergillosis - veterinary Aspergillus fumigatus Aspergillus fumigatus - pathogenicity Aspergillus fumigatus - physiology Colony Count, Microbial Culture Galactomannan Human health and pathology Infectious Disease Infectious diseases Life Sciences Lung Mannans - analysis Meleagris gallopavo Microbiology and Parasitology Mycology Parasitology Poultry Diseases - diagnosis Poultry Diseases - microbiology Poultry Diseases - mortality Poultry Diseases - pathology Real-time PCR Real-Time Polymerase Chain Reaction Spores, Fungal - pathogenicity Spores, Fungal - physiology Survival Analysis Turkey Turkeys - microbiology Turkey Galactomannan Real-time PCR Lung Aspergillosis Model Aspergillus fumigatus Culture Real-time PCR, Turkey, Lung, Aspergillosis, Galactomannan, Culture, Aspergillus fumigatus, Model
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Summary:	Abstract Aspergillus fumigatus remains a major respiratory pathogen in birds and treatment is still difficult. We challenged different groups of few-day-old turkeys via intratracheal aerosolisation with increasing concentrations (105 up to 108 ) of conidia using a MicroSprayer® device. The fungal burden was assessed by real-time PCR, galactomannan dosage, CFU counting and histopathological evaluation in order to provide a comparison of these results within each inoculum groups. Significant mortality, occurring in the first 96 h after inoculation, was only observed at the highest inoculum dose. Culture counts, GM index and qPCR results on the one hand and inoculum size on the other hand appeared to be clearly correlated. The mean fungal burden detected by qPCR was 1.3 log10 units higher than the mean values obtained by CFU measurement. The new model and the markers will be used to evaluate the efficacy of antifungal treatments that could be used in poultry farms.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0147-9571 1878-1667
DOI:	10.1016/j.cimid.2014.07.005