Genetic Markers from Biomphalaria tenagophila (Gastropoda: Pulmonata: Planorbidae) Obtained by the Double Stringency Polymerase Chain Reaction Technique

Genetic Markers from Biomphalaria tenagophila (Gastropoda: Pulmonata: Planorbidae) Obtained by the Double Stringency Polymerase Chain Reaction Technique

Biomphalaria tenagophila, one of the intermediate hosts of the trematoda Schistosoma mansoni, is a simultaneous hermafrodite snail species. In order to analyse the genetic structure of these populations, we performed a double-stringency PCR technique to obtain genetic markers with microsatellites an...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz Vol. 96; no. 3; pp. 435 - 436
Main Authors: Tuan, R, Bortolato, P C
Format: Journal Article
Language:English
Published: Brazil Fundação Oswaldo Cruz, Fiocruz 01-04-2001
Instituto Oswaldo Cruz, Ministério da Saúde
Fundação Oswaldo Cruz (FIOCRUZ)
Subjects:
Animals
Biomphalaria
Biomphalaria - genetics
Biomphalaria tenagophila
Biomphalaria, DNA polymorphism, PCR
DNA polymorphism
DNA Primers - analysis
Genetic Markers
Microsatellite Repeats - genetics
Molecular Weight
PARASITOLOGY
PCR
Polymerase Chain Reaction - methods
Polymorphism, Genetic
TROPICAL MEDICINE
PCR
Biomphalaria
DNA polymorphism
Online Access:Get full text
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Summary:Biomphalaria tenagophila, one of the intermediate hosts of the trematoda Schistosoma mansoni, is a simultaneous hermafrodite snail species. In order to analyse the genetic structure of these populations, we performed a double-stringency PCR technique to obtain genetic markers with microsatellites and arbitrary primers in a single reaction.
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ISSN:1678-8060
0074-0276
0074-0276
1678-8060
DOI:10.1590/S0074-02762001000300026