Search Results - "Borotto, Nicholas B."

The path forward for protein footprinting, covalent labeling, and mass spectrometry‐based protein conformational analyses by Borotto, Nicholas B.

“…Mass spectrometry‐based approaches to assess protein conformation have become widely utilized due to their sensitivity, low sample requirements, and broad…”
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Rapid Online Oxidation of Proteins and Peptides via Electrospray-Accelerated Ozonation by Borotto, Nicholas B., Richards, Talitha Kamakamakamae

“…Mass spectrometry-based analyses of protein conformation continue to grow in utilization due their speed, low sample requirements, and applicability to most…”
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Fragmentation and Mobility Separation of Peptide and Protein Ions in a Trapped-Ion Mobility Device by Borotto, Nicholas B, Graham, Katherine A

“…Ion mobility separations (IMS) have increasingly been coupled with mass spectrometry to increase peak capacity and deconvolute complex mass spectra in…”
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Mobility-Assisted Pseudo-MS3 Sequencing of Protein Ions by Graham, Katherine A., Grisolia, Vincent J., Borotto, Nicholas B.

“…The sequencing of intact proteins within a mass spectrometer has many benefits but is frequently limited by the fact that tandem mass spectrometry (MS/MS)…”
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Characterizing the top-down sequencing of protein ions prior to mobility separation in a timsTOF by Graham, Katherine A, Lawlor, Charles F, Borotto, Nicholas B

“…Mass spectrometry (MS)-based proteomics workflows of intact protein ions have increasingly been utilized to study biological systems. These workflows, however,…”
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Mobility-Assisted Pseudo-MS 3 Sequencing of Protein Ions by Graham, Katherine A, Grisolia, Vincent J, Borotto, Nicholas B

“…The sequencing of intact proteins within a mass spectrometer has many benefits but is frequently limited by the fact that tandem mass spectrometry (MS/MS)…”
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Collision-Induced Unfolding of Native-like Protein Ions Within a Trapped Ion Mobility Spectrometry Device by Borotto, Nicholas B, Osho, Kemi E, Richards, Talitha Kamakamakamae, Graham, Katherine A

“…Native mass spectrometry and collision-induced unfolding (CIU) workflows continue to grow in utilization due to their ability to rapidly characterize protein…”
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Gas-Phase Unfolding Reveals Stability Shifts Associated with Substrate Binding in Modular Polyketide Synthases by Zhao, Chunyi, Borotto, Nicholas B, Schmidt, Jennifer, Srivastava, Kinshuk, Lowell, Andrew, Hakansson, Kristina, Sherman, David H, Ruotolo, Brandon T

“…Native mass spectrometry (MS), ion mobility (IM), and collision-induced unfolding (CIU) have all been widely used to study the binding of small molecules to…”
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Gas-Phase Hydrogen/Deuterium Scrambling in Negative-Ion Mode Tandem Mass Spectrometry by Wang, Qingyi, Borotto, Nicholas B., Håkansson, Kristina

“…Hydrogen/deuterium exchange coupled with mass spectrometry (HDX MS) has become a powerful method to characterize protein conformational dynamics. Workflows…”
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Improved Performance of Positive-Ion Mode Free Radical-Initiated Peptide Sequencing with p‑TEMPO-Bz by Osho, Kemi E., Wasik, Kimberly A., Geary, Laina M., Borotto, Nicholas B.

“…Free radical-initiated peptide sequencing (FRIPS) is a tandem mass spectrometry technique that generates sequence informative ions via collisionally initiated…”
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Increased β‑Sheet Dynamics and D–E Loop Repositioning Are Necessary for Cu(II)-Induced Amyloid Formation by β‑2-Microglobulin by Borotto, Nicholas B, Zhang, Zhe, Dong, Jia, Burant, Brittney, Vachet, Richard W

“…β-2-Microglobulin (β2m) forms amyloid fibrils in the joints of patients undergoing dialysis treatment as a result of kidney failure. One of the ways in which…”
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Measuring the Energy Barrier of the Structural Change That Initiates Amyloid Formation by Arden, Blaise G, Borotto, Nicholas B, Burant, Brittney, Warren, William, Akiki, Christine, Vachet, Richard W

“…Obtaining kinetic and thermodynamic information for protein amyloid formation can yield new insight into the mechanistic details of this biomedically important…”
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Reversible Photochromism of 4,4’‐Disubstituted 2,2’‐Bipyridine in the Presence of SO3 by Filbin, Connor J., Haque, Md Hasanul, Locke, Cameron K., Mallon, Christopher J., Curtis, Kevin, Osho, Kemi E., Borotto, Nicholas B., Tucker, Matthew J., Odoh, Samuel O., Yang, Ying

“…We report an unusual photochromic behavior of 4,4′‐disubstituted‐2,2′‐bipyridine. It was found that in the presence of a SO3 source and HCl,…”
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Reversible Photochromism of 4,4'-Disubstituted 2,2'-Bipyridine in the Presence of SO 3 by Filbin, Connor J, Haque, Md Hasanul, Locke, Cameron K, Mallon, Christopher J, Curtis, Kevin, Osho, Kemi E, Borotto, Nicholas B, Tucker, Matthew J, Odoh, Samuel O, Yang, Ying

“…We report an unusual photochromic behavior of 4,4'-disubstituted-2,2'-bipyridine. It was found that in the presence of a SO source and HCl,…”
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Investigating Therapeutic Protein Structure with Diethylpyrocarbonate Labeling and Mass Spectrometry by Borotto, Nicholas B, Zhou, Yuping, Hollingsworth, Stephen R, Hale, John E, Graban, Eric M, Vaughan, Robert C, Vachet, Richard W

“…Protein therapeutics are rapidly transforming the pharmaceutical industry. Unlike for small molecule therapeutics, current technologies are challenged to…”
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Targeted Annotation of S‑Sulfonylated Peptides by Selective Infrared Multiphoton Dissociation Mass Spectrometry by Borotto, Nicholas B, McClory, Phillip J, Martin, Brent R, Håkansson, Kristina

“…Protein S-sulfinylation (R–SO2 –) and S-sulfonylation (R–SO3 –) are irreversible oxidative post-translational modifications of cysteine residues. Greater than…”
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Profiling Protein S‐Sulfination with Maleimide‐Linked Probes by Kuo, Yu‐Hsuan, Konopko, Aaron M., Borotto, Nicholas B., Majmudar, Jaimeen D., Haynes, Sarah E., Martin, Brent R.

“…Cysteine residues are susceptible to oxidation to form S‐sulfinyl (R‐SO2H) and S‐sulfonyl (R‐SO3H) post‐translational modifications. Here we present a simple…”
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Free Radical Initiated Peptide Sequencing for Direct Site Localization of Sulfation and Phosphorylation with Negative Ion Mode Mass Spectrometry by Borotto, Nicholas B, Ileka, Kevin M, Tom, Christina A. T. M. B, Martin, Brent R, Håkansson, Kristina

“…Tandem mass spectrometry (MS/MS) is the primary method for discovering, identifying, and localizing post-translational modifications (PTMs) in proteins…”
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Label Scrambling During CID of Covalently Labeled Peptide Ions by Borotto, Nicholas B., Degraan-Weber, Nicholas, Zhou, Yuping, Vachet, Richard W.

“…Covalent labeling along with mass spectrometry is finding more use as a means of studying the higher order structure of proteins and protein complexes…”
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Unique Effect of Cu(II) in the Metal-Induced Amyloid Formation of β‑2-Microglobulin by Dong, Jia, Joseph, Crisjoe A, Borotto, Nicholas B, Gill, Vanessa L, Maroney, Michael J, Vachet, Richard W

“…β-2-Microglobulin (β2m) forms amyloid fibrils in the joints of patients undergoing hemodialysis treatment as a result of kidney failure. In the presence of…”
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