Diagnostic DNA-flow- vs. -image-cytometry in effusion cytology

To determine the sensitivity and specificity of flow- and image-cytometry for the detection of DNA-aneuploidy as a marker for malignant cells in effusions. 200 effusions (80 tumor cell-positive, 74 negative and 46 cytologically equivocal) were stained with DAPI-SR for DNA-flow- and with Feulgen-Para...

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Published in:	Analytical cellular pathology Vol. 24; no. 1; pp. 5 - 15
Main Authors:	Motherby, Helma, Pomjanski, Natalia, Kube, Mary, Boros, Alexandra, Heiden, Thomas, Tribukait, Bernhard, Böcking, Alfred
Format:	Journal Article
Language:	English
Published:	Netherlands IOS Press 2002 Hindawi Limited
Subjects:	Algorithms Aneuploidy Ascitic Fluid - pathology Ascitic Fluid - physiopathology Biomarkers, Tumor - analysis Carcinoma - pathology Carcinoma - physiopathology Data Interpretation, Statistical DNA - analysis Epithelium - pathology Epithelium - physiopathology Flow Cytometry - instrumentation Flow Cytometry - methods Humans Image Cytometry - instrumentation Image Cytometry - methods Medicin och hälsovetenskap Mesothelioma - pathology Mesothelioma - physiopathology Other Pleural Effusion, Malignant - pathology Pleural Effusion, Malignant - physiopathology Reproducibility of Results Serous Membrane - pathology Serous Membrane - physiopathology
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Summary:	To determine the sensitivity and specificity of flow- and image-cytometry for the detection of DNA-aneuploidy as a marker for malignant cells in effusions. 200 effusions (80 tumor cell-positive, 74 negative and 46 cytologically equivocal) were stained with DAPI-SR for DNA-flow- and with Feulgen-Pararosaniline for -image-cytometry. They were measured using a PAS-flow-cytometer and an AutoCyte-QUIC-DNA-workstation according to the ESACP consensus reports for DNA-flow- and -image-cytometry, respectively [7,23,29,49]. Sensitivity of DNA-aneuploidy for the identification of malignant cells was 32.1% for DNA-flow- and 75.0% for -image-cytometry, specificity of -euploidy in benign cells was 100.0% for both methods. Positive predictive value of DNA-aneuploidy for the identification of malignant cells was 100.0% for both techniques, negative predictive value of DNA-euploidy was 48.6% for DNA-flow- and 72.0% for -image-cytometry. Searching for DNA-aneuploidy as a diagnostic marker for neoplastic cells in serous effusions image-cytometry revealed superior sensitivity as compared with monoparametric flow cytometry.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0921-8912 2210-7177 2210-7185 1878-3651
DOI:	10.1155/2002/840210