Rpv10.2 : A Haplotype Variant of Locus Rpv10 Enables New Combinations for Pyramiding Downy Mildew Resistance Traits in Grapevine

Rpv10.2 : A Haplotype Variant of Locus Rpv10 Enables New Combinations for Pyramiding Downy Mildew Resistance Traits in Grapevine

In viticulture, pathogens like the oomycete , the causal agent of downy mildew, can cause severe yield loss and require extensive application of plant protection chemicals. Breeders are generating pathogen-resistant varieties exploiting American and Asian wild germplasm as sources of resistance. Sev...

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Bibliographic Details
Published in:Plants (Basel) Vol. 13; no. 18; p. 2624
Main Authors: Höschele, Tim, Malagol, Nagarjun, Bori, Salvador Olivella, Müllner, Sophia, Töpfer, Reinhard, Sturm, Jürgen, Zyprian, Eva, Trapp, Oliver
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 20-09-2024
MDPI
Subjects:
Airborne microorganisms
Cultivars
Downy mildew
Gene mapping
Genetic analysis
Genetic aspects
Genetic crosses
Genomes
Genotype & phenotype
genotyping
Germplasm
Grapes
grapevine breeding
Haplotypes
Infections
Inoculation
Linkage analysis
Nucleotide sequence
Pathogens
phenotyping
Plant breeding
Plant protection
Population genetics
Proteins
QTL
Quantitative trait loci
Simple sequence repeats
Viticulture
Vitis
Israel
Asia
China
Germany
QTL
grapevine breeding
phenotyping
Vitis
downy mildew
genotyping
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Summary:In viticulture, pathogens like the oomycete , the causal agent of downy mildew, can cause severe yield loss and require extensive application of plant protection chemicals. Breeders are generating pathogen-resistant varieties exploiting American and Asian wild germplasm as sources of resistance. Several loci mediating resistance to have been identified in the past but may be overcome by specifically adapted strains of the pathogen. Aiming to find and characterize novel loci, a cross population with ancestry was investigated searching for resistance-correlated quantitative trait loci (QTL). As a prerequisite, a genetic map was generated by analyzing the 244 F individuals derived from a cross of the downy mildew susceptible cultivar 'Tigvoasa' and the resistant pBC1 breeding line We 90-06-12. This genetic map is based on the information from 627 molecular markers including 56 simple sequence repeats and 571 rhAmpSeq markers. A phenotypic characterization of the progeny showed a clear segregation of the resistance traits in the F population after an experimental inoculation of leaf discs with downy mildew. Combining genetic and phenotypic data, an analysis for QTL revealed a major locus on linkage Group 9 that correlates strongly with the resistance to downy mildew. The locus was mapped to a region of about 80 kb on the PN40024 (12x.V2) grapevine reference genome. This genomic region co-localizes with the formerly identified locus from the grapevine cultivar 'Solaris'. As we found different allele sizes of the locus-linked SSR markers than those characterizing the known locus and differences in the sequence of a candidate gene, it was regarded as a haplotype variant and named .
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content type line 23
ISSN:2223-7747
2223-7747
DOI:10.3390/plants13182624