Molecular genetic marker-based approaches to the verification of lilac Syringa vulgaris L. in vitro germplasm collections

RAPD analysis was used to verify the varieties in an in vitro germplasm collection of lilac Syringa vulgaris L. RAPD patterns were obtained with 16 decanucleotide primers for 46 accessions (microclones and corresponding reference varieties). The RAPD patterns of a microclone and the corresponding re...

Full description

Saved in:

Bibliographic Details
Published in:	Russian journal of genetics Vol. 45; no. 1; pp. 85 - 90
Main Authors:	Melnikova, N. V, Borhert, E. V, Martynov, S. P, Okuneva, I. B, Molkanova, O. I, Upelniek, V. P, Kudryavtsev, A. M
Format:	Journal Article
Language:	English
Published:	Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 2009 SP MAIK Nauka/Interperiodica
Subjects:	Animal Genetics and Genomics Biomedical and Life Sciences Biomedicine Human Genetics Microbial Genetics and Genomics Plant Genetics Syringa vulgaris Relative Genetic Distance Replication Number Reference Variety Genetic Distance Germplasm Collection
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	RAPD analysis was used to verify the varieties in an in vitro germplasm collection of lilac Syringa vulgaris L. RAPD patterns were obtained with 16 decanucleotide primers for 46 accessions (microclones and corresponding reference varieties). The RAPD patterns of a microclone and the corresponding reference variety often differed in composition; consequently, it was infeasible to verify the accessions by direct comparison of the RAPD patterns. Hence, evaluation of the relative genetic distances between accessions (microclones) and known varieties was proposed as a method to verify lilac in vitro germplasm collections.
Bibliography:	http://dx.doi.org/10.1134/S1022795409010128 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	1022-7954 1608-3369
DOI:	10.1134/S1022795409010128