Analysis of microtubule cytoskeleton distribution using a fluorescent taxoid in two trichomonadid protozoa: Trichomonas gallinae and Tritrichomonas foetus

Trichomonas gallinae and Tritrichomonas foetus are flagellated parasitic protozoa of the upper digestive tract of birds and the urogenital tract of cattle, respectively. Both of these species are important in the veterinary field, due to the fact that they cause significant economic losses. Therefor...

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Bibliographic Details
Published in:	Experimental parasitology Vol. 119; no. 1; pp. 186 - 191
Main Authors:	Vieira, P.B., Borges, F.P., Gottardi, B., Stuepp, C., Larré, A.B., Tasca, T., De Carli, G.A.
Format:	Journal Article
Language:	English
Published:	San Diego, CA Elsevier Inc 01-05-2008 Elsevier
Subjects:	Animals binding sites Biological and medical sciences Cattle Columbidae cytoskeleton Cytoskeleton - ultrastructure diterpenoids Fluorescent Dyes - metabolism fluorescent labeling Fluorescent taxoid FLUTAX-2 Fundamental and applied biological sciences. Psychology Life cycle. Host-agent relationship. Pathogenesis Microscopy, Fluorescence Microtubule cytoskeleton microtubules Microtubules - metabolism Microtubules - ultrastructure Protozoa spatial distribution Taxoids - metabolism Trichomonas - ultrastructure Trichomonas gallinae Tritrichomonas foetus Tritrichomonas foetus - ultrastructure trophozoites tubulin ultrastructure Trichomonas gallinae Microtubule cytoskeleton Fluorescent taxoid FLUTAX-2 Tritrichomonas foetus Protozoa Parasite Cytoskeleton Microtubule Trichomonadida
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Summary:	Trichomonas gallinae and Tritrichomonas foetus are flagellated parasitic protozoa of the upper digestive tract of birds and the urogenital tract of cattle, respectively. Both of these species are important in the veterinary field, due to the fact that they cause significant economic losses. Therefore, we investigated the morphology of these parasites by studying microtubule cytoskeleton organization. FLUTAX-2, an active fluorescent derivative of Taxol, was used in this study. This fluorescent taxoid binds to polymerized αβ-tubulin dimers. Our results showed that FLUTAX-2 was able to bind to and stabilize microtubules of intact T. gallinae and T. foetus trophozoites, allowing the microtubular cytoskeleton to be easily observed by fluorescence microscopy. T. foetus and T. gallinae had no differences in their FLUTAX-2 binding profiles. Further studies may allow this technique to be improved, and it may possibly be used as a routine laboratory method for the diagnosis of avian and bovine trichomonosis.
Bibliography:	http://dx.doi.org/10.1016/j.exppara.2007.12.011 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-4894 1090-2449
DOI:	10.1016/j.exppara.2007.12.011