Relationship between biomarker expression and allelic alteration in esophageal carcinoma

Background and Aim: Expression of biomarkers and probable allelic alterations were studied in esophagus tissue samples from patients with esophageal carcinoma. Methods: A total of 116 esophagus tissue samples were obtained from 25 patients with esophagus cancer. Histological studies revealed 23 sa...

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Bibliographic Details
Published in:	Journal of gastroenterology and hepatology Vol. 22; no. 12; pp. 2303 - 2309
Main Authors:	Vegh, Irene, De-La-Calle Santiuste, Angel, Colina, Francisco, Bor, Lázsló, Bermejo, Clara, Aragón, Ana, Morán-Jiménez, María-José, Gómez-Cámara, Agustín, De Salamanca, Rafael Enríquez, Moreno-González, Enrique
Format:	Journal Article
Language:	English
Published:	Melbourne, Australia Blackwell Publishing Asia 01-12-2007 Blackwell Science
Subjects:	Adenocarcinoma - enzymology Alleles allelic alteration Biological and medical sciences biomarker Biomarkers, Tumor - metabolism Carcinoembryonic Antigen - metabolism Carcinoma, Squamous Cell - enzymology chromosome 17p Esophageal Neoplasms - enzymology Esophageal Neoplasms - genetics Esophageal Neoplasms - metabolism Esophagus esophagus tumor Female Gastroenterology. Liver. Pancreas. Abdomen Humans Lymph Nodes - pathology Male Matrix Metalloproteinase 1 - metabolism Medical sciences Middle Aged Receptor, Epidermal Growth Factor - metabolism Tissue Inhibitor of Metalloproteinase-1 - metabolism Tumors esophagus tumor Esophageal disease Biological marker Digestive diseases Chromosome Esophagus carcinoma Malignant tumor biomarker chromosome 17p allelic altération Cancer Esophagus
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Summary:	Background and Aim: Expression of biomarkers and probable allelic alterations were studied in esophagus tissue samples from patients with esophageal carcinoma. Methods: A total of 116 esophagus tissue samples were obtained from 25 patients with esophagus cancer. Histological studies revealed 23 samples were adenocarcinoma and 14 samples were epidermoid carcinoma while 79 samples were non‐tumor. Expression of biomarkers was determined by enzyme immunoassay, and allelic alterations on chromosome 17p were performed by polymerase chain reaction (PCR) using primers D17S513 and D17S514. Results: The adenocarcinoma group exhibited an increase of matrix metalloproteinase (MMP)‐1 (P < 0.0001) and sialyl Le (a) (P < 0.001) mean levels when compared with the non‐tumor group. Adenocarcinoma samples from patients with more than three positive lymph nodes had lower levels of tissue‐inhibitor metalloproteinase (TIMP)‐1 than those with negative nodes (P < 0.0005). Positive allelic alteration was associated with high levels of MMP‐1 expression (P = 0.003). Epidermoid carcinoma samples showed higher expression of MMP‐1 (P < 0.0001) and TIMP‐1 (P < 0.02) than non‐tumor samples. Both epidermal growth factor receptor and sialyl Le (a) levels were overexpressed in tumors of patients with more than three positive lymph nodes (P < 0.005). Carcinoembryonic antigen levels were higher in tumors associated with allelic wild type group (P = 0.0001) and patients with negative lymph nodes (P < 0.05). Furthermore, variability in expression of biomarkers was observed according to sample location, and allelic alterations were also found both in tumor and in some non‐tumor samples. Conclusion: The data suggest that overexpression of tissue biomarkers associated with allelic alterations may have potential prognostic implications with different behavior in esophagus cancer.
Bibliography:	ark:/67375/WNG-1M2DNHV0-C ArticleID:JGH4374 istex:0553075DCF5A6F1D33E3369E9EB0A92BB524540F ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0815-9319 1440-1746
DOI:	10.1111/j.1440-1746.2006.04374.x