Search Results - "Boocock, Martin R."

Structural basis for topological regulation of Tn3 resolvase by Montaño, Sherwin P, Rowland, Sally-J, Fuller, James R, Burke, Mary E, MacDonald, Alasdair I, Boocock, Martin R, Stark, W Marshall, Rice, Phoebe A

“…Site-specific DNA recombinases play a variety of biological roles, often related to the dissemination of antibiotic resistance, and are also useful synthetic…”
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Roles of two large serine recombinases in mobilizing the methicillin‐resistance cassette SCCmec by Misiura, Agnieszka, Pigli, Ying Z., Boyle‐Vavra, Susan, Daum, Robert S., Boocock, Martin R., Rice, Phoebe A.

“…Summary Methicillin‐resistant Staphylococcus aureus (MRSA) emerged via acquisition of a mobile element, staphylococcal cassette chromosome mec (SCCmec)…”
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Structural Basis for Catalytic Activation of a Serine Recombinase by Keenholtz, Ross A., Rowland, Sally-J., Boocock, Martin R., Stark, W. Marshall, Rice, Phoebe A.

“…Sin resolvase is a site-specific serine recombinase that is normally controlled by a complex regulatory mechanism. A single mutation, Q115R, allows the enzyme…”
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Arginine as a General Acid Catalyst in Serine Recombinase-mediated DNA Cleavage by Keenholtz, Ross A., Mouw, Kent W., Boocock, Martin R., Li, Nan-Sheng, Piccirilli, Joseph A., Rice, Phoebe A.

“…Sin resolvase is a site-specific DNA recombinase that catalyzes phosphotransfer without the use of divalent cations. Results: Mutation of Arg-69 in the active…”
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Intermediates in serine recombinase-mediated site-specific recombination by Marshall Stark, W, Boocock, Martin R, Olorunniji, Femi J, Rowland, Sally-J

“…Site-specific recombinases are enzymes that promote precise rearrangements of DNA sequences. They do this by cutting and rejoining the DNA strands at specific…”
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Chimeric Recombinases with Designed DNA Sequence Recognition by Akopian, Aram, He, Jiuya, Boocock, Martin R., Stark, W. Marshall

“…Site-specific recombination typically occurs only between DNA sequences that have co-evolved with a natural recombinase enzyme to optimize sequence…”
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Orchestrating serine resolvases by Rice, Phoebe A, Mouw, Kent W, Montaño, Sherwin P, Boocock, Martin R, Rowland, Sally-J, Stark, W Marshall

“…A remarkable feature of the serine resolvases is their regulation: the wild-type enzymes will catalyse intra- but not inter-molecular recombination, can sense…”
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The protein–protein interactions required for assembly of the Tn3 resolution synapse by Rowland, Sally‐J., Boocock, Martin R., Burke, Mary E., Rice, Phoebe A., Stark, W. Marshall

“…The site‐specific recombinase Tn3 resolvase initiates DNA strand exchange when two res recombination sites and six resolvase dimers interact to form a synapse…”
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Snapshots of a molecular swivel in action by Trejo, Caitlin S, Rock, Ronald S, Stark, W Marshall, Boocock, Martin R, Rice, Phoebe A

“…Abstract Members of the serine family of site-specific recombinases exchange DNA strands via 180° rotation about a central protein-protein interface. Modeling…”
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A proposed mechanism for IS607-family serine transposases by Boocock, Martin R, Rice, Phoebe A

“…The transposases encoded by the IS607 family of mobile elements are unusual serine recombinases with an inverted domain order and minimal specificity for…”
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Architecture of a Serine Recombinase-DNA Regulatory Complex by Mouw, Kent W., Rowland, Sally-J., Gajjar, Mark M., Boocock, Martin R., Stark, W. Marshall, Rice, Phoebe A.

“…An essential feature of many site-specific recombination systems is their ability to regulate the direction and topology of recombination. Resolvases from the…”
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Regulatory mutations in Sin recombinase support a structure-based model of the synaptosome by Rowland, Sally-J, Boocock, Martin R, McPherson, Arlene L, Mouw, Kent W, Rice, Phoebe A, Stark, W. Marshall

“…The resolvase Sin regulates DNA strand exchange by assembling an elaborate interwound synaptosome containing catalytic and regulatory Sin tetramers, and an…”
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Sin Resolvase Catalytic Activity and Oligomerization State are Tightly Coupled by Mouw, Kent W., Steiner, Andrew M., Ghirlando, Rodolfo, Li, Nan-Sheng, Rowland, Sally-J., Boocock, Martin R., Stark, W. Marshall, Piccirilli, Joseph A., Rice, Phoebe A.

“…Serine recombinases promote specific DNA rearrangements by a cut-and-paste mechanism that involves cleavage of all four DNA strands at two sites recognized by…”
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Synapsis and catalysis by activated Tn3 resolvase mutants by Olorunniji, Femi J., He, Jiuya, Wenwieser, Sandra V.C.T., Boocock, Martin R., Stark, W. Marshall

“…The serine recombinase Tn3 resolvase catalyses recombination between two 114 bp res sites, each of which contains binding sites for three resolvase dimers. We…”
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Implications of Structures of Synaptic Tetramers of γδ Resolvase for the Mechanism of Recombination by Kamtekar, Satwik, Ho, Roger S., Cocco, Melanie J., Li, Weikai, Wenwieser, Sandra V. C. T., Boocock, Martin R., Grindley, Nigel D. F., Steitz, Thomas A.

“…The structures of two mutants of the site-specific recombinase, γδ resolvase, that form activated tetramers have been determined. One, at 3.5-Å resolution,…”
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Implications of structures of synaptic tetramers of gamma delta resolvase for the mechanism of recombination by Kamtekar, Satwik, Ho, Roger S, Cocco, Melanie J, Li, Weikai, Wenwieser, Sandra V C T, Boocock, Martin R, Grindley, Nigel D F, Steitz, Thomas A

“…The structures of two mutants of the site-specific recombinase, gammadelta resolvase, that form activated tetramers have been determined. One, at 3.5-A…”
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A Model for the γδ Resolvase Synaptic Complex by Sarkis, Gary J, Murley, Laura Lea, Leschziner, Andres E, Boocock, Martin R, Stark, W.Marshall, Grindley, Nigel D.F

“…The serine recombinase γδ resolvase performs site-specific recombination in an elaborate synaptic complex containing 12 resolvase subunits and two 114-base…”
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Regulation of Sin recombinase by accessory proteins by Rowland, Sally J., Boocock, Martin R., Stark, W. Marshall

“…Summary Sin recombinase from Staphylococcus aureus acts selectively on directly repeated resH sites, assembling an intertwined synapse in which exactly three…”
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Activating mutations of Tn3 resolvase marking interfaces important in recombination catalysis and its regulation by Burke, Mary E., Arnold, Patricia H., He, Jiuya, Wenwieser, Sandra V. C. T., Rowland, Sally‐J., Boocock, Martin R., Stark, W. Marshall

“…Summary Catalysis of DNA recombination by Tn3 resolvase is conditional on prior formation of a synapse, comprising 12 resolvase subunits and two recombination…”
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DNA bending in the Sin recombination synapse: functional replacement of HU by IHF by Rowland, Sally‐J., Boocock, Martin R., Stark, W. Marshall

“…Summary The serine recombinase Sin requires a non‐specific DNA‐bending protein such as Hbsu for activity at its recombination site resH. Hbsu, and Sin subunits…”
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