A novel computerised image analysis method for the measurement of production of conidia from the aphid pathogenic fungus Erynia neoaphidis

A novel computerised image analysis method for the measurement of production of conidia from the aphid pathogenic fungus Erynia neoaphidis

A semi-automated method has been developed for the quantification and measurement of conidia discharged by the aphid pathogen Erynia neoaphidis. This was used to compare conidiation by E. neoaphidis-mycosed pea aphid cadavers, mycelial plugs cut from agar plates, mycelial pellets from shake flasks a...

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Bibliographic Details
Published in:FEMS microbiology letters Vol. 220; no. 1; pp. 75 - 80
Main Authors: Bonner, Tony J., Pell, Judith K., Gray, Simon N.
Format: Journal Article
Language:English
Published: Oxford, UK Elsevier B.V 14-03-2003
Blackwell Publishing Ltd
Blackwell
Subjects:
Action of physical and chemical agents
Acyrthosiphon pisum
agar
Animals
Aphids - microbiology
Automation
Biological and medical sciences
Biological control
biological control agents
Biomass
Cell Size
conidia
conidiation
containers
Entomophthorales - physiology
Entomophthorales - ultrastructure
Erynia neoaphidis
Fermentation
fermenters
Fundamental and applied biological sciences. Psychology
fungi
Image analysis
Image Processing, Computer-Assisted - methods
Microbiology
mycelium
Mycological methods and techniques used in mycology
Mycology
pathogens
pellets
Pest Control, Biological
Production and dimensions of conidia
Spores, Fungal - ultrastructure
Virology
Image analysis
Production and dimensions of conidia
Biological control
Erynia neoaphidis
Pathogenicity
Aphidoidea
Homoptera
Arthropoda
Insecta
Aphididae
Method
Invertebrata
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Summary:A semi-automated method has been developed for the quantification and measurement of conidia discharged by the aphid pathogen Erynia neoaphidis. This was used to compare conidiation by E. neoaphidis-mycosed pea aphid cadavers, mycelial plugs cut from agar plates, mycelial pellets from shake flasks and by mycelial pellets from different phases of liquid batch fermenter culture. Aphid cadavers discharged significantly more and significantly smaller conidia than plugs or pellets. The volume of conidia discharged was stable over the period of discharge (80 h), but more detailed analysis of the size frequency distribution showed that more very small and very large conidia were discharged after 5 h incubation than after 75 h incubation. Biomass harvested at the end of the exponential growth phase in batch fermenter culture produced significantly more conidia than biomass from any other growth phase. The implications of these findings for the development of production and formulation processes for E. neoaphidis as a biological control agent are discussed.
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ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(03)00063-6