Treatment of critical defects produced in calvaria of mice with mesenchymal stem cells

Treatment of critical defects produced in calvaria of mice with mesenchymal stem cells

Mesenchymal stem cells (MSC) are present in specialized niches in perivascular regions of adult tissues and are able to differentiate into various cell types, such as those committed to repairing. Bone marrow derived MSC from eight young mice C57BL/ 6 gfp(+) were expanded in culture for repairing cr...

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Bibliographic Details
Published in:Anais da Academia Brasileira de Ciências Vol. 84; no. 3; pp. 841 - 851
Main Authors: Monteiro, Betânia S, Argôlo-Neto, Napoleão M, Nardi, Nance B, Chagastelles, Pedro C, Carvalho, Pablo H, Bonfá, Laila P, Filgueiras, Richard R, Reis, Amanda S, Del Carlo, Ricardo J
Format: Journal Article
Language:English
Published: Brazil Academia Brasileira de Ciências 01-09-2012
Subjects:
Animals
Autologous graft
Bone Regeneration - physiology
Bone therapy
Calvaria
calvária
camundongo
Cells, Cultured
células-tronco mesenquimais
enxerto autólogo
Male
Mesenchymal stem cell
Mesenchymal Stem Cell Transplantation - methods
Mice
Mice, Inbred C57BL
MULTIDISCIPLINARY SCIENCES
Osteogenesis - physiology
Random Allocation
Skull - injuries
Skull - surgery
terapia óssea
Tissue Engineering
Mesenchymal stem cell
Calvaria
calvária
Bone therapy
enxerto autólogo
Autologous graft
células-tronco mesenquimais
Mice
camundongo
terapia óssea
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Summary:Mesenchymal stem cells (MSC) are present in specialized niches in perivascular regions of adult tissues and are able to differentiate into various cell types, such as those committed to repairing. Bone marrow derived MSC from eight young mice C57BL/ 6 gfp(+) were expanded in culture for repairing critical defects in calvarial bone produced in twenty-four young isogenic adult C57BL/6 mice. The animals were subjected to a cranial defect of 6.0mm diameter and divided into two equal experimental groups. Control group did not receive any treatment and the treated group received a MSC pellet containing 1.0 x 10(7) cells/mL into the defects. The group treated with MSC showed increased angiogenesis and amount of new bone deposited on the defect limits than that observed in the control group. The results demonstrated that transplantation of bone marrow-derived MSC of C57BL/6 gfp(+) mice to bone critical defects produced in mice calvarial contributes positively to the bone repair process. MSC presets ability to influence the correct functioning of osteoblasts, increases the amount of mobilized cells for the repairing process, speeds up growth, and increases deposition of bone matrix.
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ISSN:0001-3765
1678-2690
1678-2690
0001-3765
DOI:10.1590/S0001-37652012000300026