Radioprotective effect of Haberlea rhodopensis (Friv.) leaf extract on gamma-radiation-induced DNA damage, lipid peroxidation and antioxidant levels in rabbit blood

Radioprotective effect of Haberlea rhodopensis (Friv.) leaf extract on gamma-radiation-induced DNA damage, lipid peroxidation and antioxidant levels in rabbit blood

Different concentrations of H. rhodopensis total extract (HRE; 0.03, 0.06 and 0.12 g/kg body weight) were injected im, into rabbits 2 h before collecting the blood samples. The whole blood samples were exposed in vitro to 2.0 Gy 60Co gamma-radiation. The radiation-induced changes were estimated by u...

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Bibliographic Details
Published in:Indian journal of experimental biology Vol. 51; no. 1; pp. 29 - 36
Main Authors: Georgieva, Svetlana, Popov, Borislav, Bonev, Georgi
Format: Journal Article
Language:English
Published: India 01-01-2013
Subjects:
Animals
Antioxidants - chemistry
Antioxidants - metabolism
Catalase - metabolism
Chromosome Aberrations
Culture Media
DNA Damage - drug effects
Erythrocytes - cytology
Free Radicals
Gamma Rays
Lipid Peroxidation
Magnoliopsida - metabolism
Male
Micronucleus Tests - methods
Models, Biological
Plant Extracts - pharmacology
Rabbits
Radiation Injuries - prevention & control
Radiation-Protective Agents - pharmacology
Superoxide Dismutase - metabolism
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Summary:Different concentrations of H. rhodopensis total extract (HRE; 0.03, 0.06 and 0.12 g/kg body weight) were injected im, into rabbits 2 h before collecting the blood samples. The whole blood samples were exposed in vitro to 2.0 Gy 60Co gamma-radiation. The radiation-induced changes were estimated by using the chromosome aberration test (CA) and cytokinesis blocked micronucleus assay (CBMN) in peripheral lymphocytes, and by determining the malondialdehyde levels (MDA) in blood plasma and the superoxide dismutase (SOD) and catalase (CAT) activity in erythrocytes. Radiation significantly increased the chromosome aberration and micronuclei frequencies as well as MDA levels and decreased the antioxidant enzyme activity. On the other hand, the HRE pretreatment significantly decreased the CA, MN frequencies and MDA levels and increased the SOD and CAT activity in a concentration dependent manner. The most effective was the highest concentration of HRE (0.12 g/kg body weight). The results suggest that HRE as a natural product with a nantioxidant capacity could play a modulatory role against the cellular damage induced by gamma-irradiation. The possible mechanism involved in the radioprotective potential of HRE is discussed.
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ISSN:0019-5189