Sarcocystis neurona: Molecular characterization of enolase domain I region and a comparison to other protozoa

Sarcocystis neurona causes protozoal myeloencephalitis and has the ability to infect a wide host range in contrast to other Sarcocystis species. In the current study, five S. neurona isolates from a variety of sources, three Sarcocystis falcatula, one Sarcocystis dasypi/S. neurona-like isolate, and...

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Bibliographic Details
Published in:	Experimental parasitology Vol. 120; no. 1; pp. 108 - 112
Main Authors:	Bolten, K.E., Marsh, A.E., Reed, S.M., Dubey, J.P., Toribio, R.E., Saville, W.J.A.
Format:	Journal Article
Language:	English
Published:	San Diego, CA Elsevier Inc 01-09-2008 Elsevier
Subjects:	Amino Acid Sequence amino acid sequences Animals Armadillos Base Sequence Besnoitia darlingi Biological and medical sciences conserved sequences developmental stages DNA, Protozoan - chemistry Enolase Fundamental and applied biological sciences. Psychology gene expression genes Horses hydro-lyases Immunoblotting - veterinary immunohistochemistry Immunohistochemistry - veterinary Life cycle. Host-agent relationship. Pathogenesis Mice Mice, Knockout Molecular Sequence Data Neospora caninum Neospora hughesi nucleotide sequences Opossums parasites Phosphopyruvate Hydratase - chemistry Phosphopyruvate Hydratase - genetics Phosphopyruvate Hydratase - immunology phylogeny Plasmodium falciparum Protozoa Protozoal myeloencephalitis Sarcocystidae Sarcocystis - enzymology Sarcocystis - genetics Sarcocystis - immunology Sarcocystis dasypi Sarcocystis falcatula Sarcocystis neurona Sequence Alignment - veterinary sequence analysis sequence homology Toxoplasma gondii Trypanosoma cruzi Sarcocystis dasypi Protozoal myeloencephalitis Sarcocystis neurona Besnoitia darlingi Sarcocystis falcatula Enolase Protozoa Apicomplexa Parasite Sarcocystis Comparative study
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Summary:	Sarcocystis neurona causes protozoal myeloencephalitis and has the ability to infect a wide host range in contrast to other Sarcocystis species. In the current study, five S. neurona isolates from a variety of sources, three Sarcocystis falcatula, one Sarcocystis dasypi/S. neurona-like isolate, and one Besnoitia darlingi isolate were used to compare the enolase 2 gene segment containing the domain I region to previously sequenced enolase genes from Neospora caninum, Neospora hughesi, Toxoplasma gondii, Plasmodium falciparum, and Trypanosoma cruzi; enolase 2 segment containing domain I region is highly conserved amongst these parasites of veterinary and medical importance. Immunohistochemistry results indicates reactivity of T. gondii enolase 1 and 2 antibodies to S. neurona merozoites and metrocytes, but no reactivity of anti-enolase 1 to the S. neurona bradyzoite stage despite reactivity to T. gondii bradyzoites, suggesting expression differences between organisms.
Bibliography:	http://dx.doi.org/10.1016/j.exppara.2008.05.004 http://hdl.handle.net/10113/20793 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-4894 1090-2449
DOI:	10.1016/j.exppara.2008.05.004