Search Results - "Boles, Jeffrey O"
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Wipe sampling of amphetamine-type stimulants and recreational drugs on selected household surfaces with analysis by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry
Published in Journal of hazardous materials (15-06-2013)“…•Degree of sorption of eight drugs on eleven countertop surfaces was investigated.•Surface composition, volatility and solvent composition played a role in…”
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Identification and biochemical characterization of the ligand binding domain of the collagen adhesin from Staphylococcus aureus
Published in Biochemistry (Easton) (01-10-1993)“…We have recently shown that the expression of a collagen adhesin is both necessary and sufficient to mediate the attachment of Staphylococcus aureus to…”
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Brownian Dynamics of Cytochrome c and Cytochrome c Peroxidase Association
Published in Science (American Association for the Advancement of Science) (01-07-1988)“…Brownian dynamics computer simulations of the diffusional association of electron transport proteins cytochrome c (cyt c) and cytochrome c peroxidase (cyt c…”
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Synthesis and incorporation of [6,7]-selenatryptophan into dihydrofolate reductase
Published in Biochemical and biophysical research communications (25-10-2002)“…Until recently, the only selenium containing amino acid which could be used to completely substitute for a wild type amino acid was selenomethionine (SeMet)…”
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Assessing Cell Growth in a Microtiter Plate Reader with Selective Pressure Incorporation of Unnatural Selenium and Tellurium Containing Amino Acids
Published in The FASEB journal (01-04-2019)“…Metalloid‐containing unnatural amino acids, when incorporated into target proteins, provide a means of solving for three‐dimensional structure by MAD or SAD…”
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Critical Residues in the Ligand-binding Site of the Staphylococcus aureus Collagen-binding Adhesin (MSCRAMM)
Published in The Journal of biological chemistry (19-05-1995)“…We have identified a discrete collagen-binding site within the Staphylococcus aureus collagen adhesin that is located in a region between amino acids Asp209…”
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Analysis of differentially expressed proteins in Escherichia coli when exposed to L‐Telluromethionine
Published in The FASEB journal (01-04-2013)“…Abstract only Analysis of the three‐dimensional structure of a protein using x‐ray crystallography can be improved by the incorporation of unnatural amino…”
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Bio-incorporation of telluromethionine into buried residues of dihydrofolate reductase
Published in Nature structural biology (01-05-1994)Get full text
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Enzymatic and mass spectral analysis of mutant and wild‐type selenomethionyl‐dihydrofolate reductase
Published in The FASEB journal (01-04-2011)“…The biosynthetic incorporation of unnatural amino acids, primarily selenomethionine, has been used for over a decade to facilitate structural determination of…”
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ANALYSIS OF DIFFERENTIALLY EXPRESSED PROTEINS IN ESCHERICHIA COLI WHEN EXPOSED TO L‐TELLUROMETHIONINE
Published in The FASEB journal (01-04-2012)“…Abstract only Analyzing the three‐dimensional structure of a protein using x‐ray crystallography can be improved by incorporating unnatural amino acids into…”
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Proteomic profiling of unnatural amino acid toxicity through differential expression of wild type vs. telluroproteins in Eschericia coli DL41(DE3)
Published in The FASEB journal (01-04-2010)“…Three‐dimensional conformation of proteins can be solved with greater ease by utilizing unnatural amino acids (UAA), along with x‐ray crystallography. Because…”
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Tellurite Resistance Transformation for Simplified X‐Ray Crystallographic Protein Structure Analysis
Published in The FASEB journal (01-04-2010)“…One of the most common techniques for determining protein structure is X‐ray crystallography using selenium‐substituted amino acids. The disadvantage of this…”
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DNA Analysis Using Polymerase Chain Reactions and Micro‐Electrophoresis
Published in The FASEB journal (01-04-2010)“…Polymerase Chain Reaction (PCR) is used in a variety of applications in forensics, medicine, and research. PCR relies on the ability to thermally break apart a…”
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Purification and characterization of selenomethionyl thymidylate synthase from Escherichia coli: comparison with the wild-type enzyme
Published in Biochemistry (Easton) (19-11-1991)“…Replacement of methionine (Met) residues by selenomethionine (SeMet) was recently shown to facilitate the crystallographic analysis of protein structure…”
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Diffusion-controlled association rate of cytochrome c and cytochrome c peroxidase in a simple electrostatic model
Published in Journal of the American Chemical Society (01-12-1986)Get full text
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Electrostatic effects in the Brownian dynamics of association and orientation of heme proteins
Published in Journal of physical chemistry (1952) (01-11-1987)Get full text
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Isolation and characterization of pcp, a gene encoding a pyrrolidone carboxyl peptidase in Staphylococcus aureus
Published in Gene (01-12-1995)“…The pcp gene, encoding a pyrrolidone carboxyl peptidase (PYRase), was cloned from a γGTll genomic library prepared from Staphylococcus aureus FDA 574 and…”
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Brownian dynamics simulation of protein association
Published in Journal of computer-aided molecular design (01-01-1988)“…The Brownian Dynamics (BD) method is applied to study the diffusive dynamics and interaction of two proteins, cytochrome c (CYTC) and cytochrome c peroxidase…”
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