Search Results - "Boles, Jeffrey O"

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    Identification and biochemical characterization of the ligand binding domain of the collagen adhesin from Staphylococcus aureus by Patti, Joseph M, Boles, Jeffrey O, Hook, Magnus

    Published in Biochemistry (Easton) (01-10-1993)
    “…We have recently shown that the expression of a collagen adhesin is both necessary and sufficient to mediate the attachment of Staphylococcus aureus to…”
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    Journal Article
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    Brownian Dynamics of Cytochrome c and Cytochrome c Peroxidase Association by Northrup, Scott H., Boles, Jeffrey O., John C. L. Reynolds

    “…Brownian dynamics computer simulations of the diffusional association of electron transport proteins cytochrome c (cyt c) and cytochrome c peroxidase (cyt c…”
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    Journal Article
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    Synthesis and incorporation of [6,7]-selenatryptophan into dihydrofolate reductase by Boles, Jeffrey O, Henderson, James, Hatch, Duane, Silks, Louis A.“Pete”

    “…Until recently, the only selenium containing amino acid which could be used to completely substitute for a wild type amino acid was selenomethionine (SeMet)…”
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    Assessing Cell Growth in a Microtiter Plate Reader with Selective Pressure Incorporation of Unnatural Selenium and Tellurium Containing Amino Acids by Brown, Amy, Hill, Austin R., Boles, Jeffrey O.

    Published in The FASEB journal (01-04-2019)
    “…Metalloid‐containing unnatural amino acids, when incorporated into target proteins, provide a means of solving for three‐dimensional structure by MAD or SAD…”
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    Critical Residues in the Ligand-binding Site of the Staphylococcus aureus Collagen-binding Adhesin (MSCRAMM) by Patti, Joseph M., House-Pompeo, Karen, Boles, Jeffrey O., Garza, Norma, Gurusiddappa, S., Höök, Magnus

    Published in The Journal of biological chemistry (19-05-1995)
    “…We have identified a discrete collagen-binding site within the Staphylococcus aureus collagen adhesin that is located in a region between amino acids Asp209…”
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    Analysis of differentially expressed proteins in Escherichia coli when exposed to L‐Telluromethionine by Broderick, Kathleen Marie, Taylor, Savannah, Boles, Jeffrey O.

    Published in The FASEB journal (01-04-2013)
    “…Abstract only Analysis of the three‐dimensional structure of a protein using x‐ray crystallography can be improved by the incorporation of unnatural amino…”
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    Enzymatic and mass spectral analysis of mutant and wild‐type selenomethionyl‐dihydrofolate reductase by Broderick, Kathleen Marie, Boles, Jeffrey O

    Published in The FASEB journal (01-04-2011)
    “…The biosynthetic incorporation of unnatural amino acids, primarily selenomethionine, has been used for over a decade to facilitate structural determination of…”
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    Journal Article
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    ANALYSIS OF DIFFERENTIALLY EXPRESSED PROTEINS IN ESCHERICHIA COLI WHEN EXPOSED TO L‐TELLUROMETHIONINE by Broderick, Kathleen Marie, Boles, Jeffrey O, Brinkley, Sarah

    Published in The FASEB journal (01-04-2012)
    “…Abstract only Analyzing the three‐dimensional structure of a protein using x‐ray crystallography can be improved by incorporating unnatural amino acids into…”
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    Proteomic profiling of unnatural amino acid toxicity through differential expression of wild type vs. telluroproteins in Eschericia coli DL41(DE3) by Brinkley, Sarah Christine, Boles, Jeffrey O

    Published in The FASEB journal (01-04-2010)
    “…Three‐dimensional conformation of proteins can be solved with greater ease by utilizing unnatural amino acids (UAA), along with x‐ray crystallography. Because…”
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    Journal Article
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    Tellurite Resistance Transformation for Simplified X‐Ray Crystallographic Protein Structure Analysis by Mancuso, Matthew Cian, Boles, Jeffrey O

    Published in The FASEB journal (01-04-2010)
    “…One of the most common techniques for determining protein structure is X‐ray crystallography using selenium‐substituted amino acids. The disadvantage of this…”
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    Journal Article
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    DNA Analysis Using Polymerase Chain Reactions and Micro‐Electrophoresis by Hunley, Ryan Timothy, Harwood, John, Boles, Jeffrey O., Mancuso, Matt

    Published in The FASEB journal (01-04-2010)
    “…Polymerase Chain Reaction (PCR) is used in a variety of applications in forensics, medicine, and research. PCR relies on the ability to thermally break apart a…”
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    Purification and characterization of selenomethionyl thymidylate synthase from Escherichia coli: comparison with the wild-type enzyme by Boles, Jeffrey O, Cisneros, Ralph J, Weir, Mary S, Odom, Jerome D, Villafranca, J. E, Dunlap, R. Bruce

    Published in Biochemistry (Easton) (19-11-1991)
    “…Replacement of methionine (Met) residues by selenomethionine (SeMet) was recently shown to facilitate the crystallographic analysis of protein structure…”
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    Isolation and characterization of pcp, a gene encoding a pyrrolidone carboxyl peptidase in Staphylococcus aureus by Patti, Joseph M., Schneider, Amy, Garza, Norma, Boles, Jeffrey O.

    Published in Gene (01-12-1995)
    “…The pcp gene, encoding a pyrrolidone carboxyl peptidase (PYRase), was cloned from a γGTll genomic library prepared from Staphylococcus aureus FDA 574 and…”
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    Brownian dynamics simulation of protein association by Northrup, S H, Luton, J A, Boles, J O, Reynolds, J C

    Published in Journal of computer-aided molecular design (01-01-1988)
    “…The Brownian Dynamics (BD) method is applied to study the diffusive dynamics and interaction of two proteins, cytochrome c (CYTC) and cytochrome c peroxidase…”
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    Journal Article