Isolation and Characterization of a Novel Klebsiella pneumoniae N4-like Bacteriophage KP8

is a common pathogen, associated with a wide spectrum of infections, and clinical isolates of often possess multiple antibiotic resistances. Here, we describe a novel lytic N4-like bacteriophage KP8, specific to , including its genome, partial structural proteome, biological properties, and proposed...

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Bibliographic Details
Published in:	Viruses Vol. 11; no. 12; p. 1115
Main Authors:	Morozova, Vera, Babkin, Igor, Kozlova, Yuliya, Baykov, Ivan, Bokovaya, Olga, Tikunov, Artem, Ushakova, Tatyana, Bardasheva, Alevtina, Ryabchikova, Elena, Zelentsova, Ekaterina, Tikunova, Nina
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 02-12-2019 MDPI
Subjects:	Bacteriophage T7 Bacteriophages - classification Bacteriophages - isolation & purification Bacteriophages - physiology Bacteriophages - ultrastructure Clinical isolates Divergence DNA, Viral DNA-directed RNA polymerase Drug resistance Electron microscopy Genes Genome, Viral Genomes Genomics Host Specificity Klebsiella pneumoniae Klebsiella pneumoniae - virology Morphology New genera Nosocomial infections Open reading frames Phages Phylogenetics Phylogeny Polyethylene glycol Proteins Proteomes Synteny Viral Plaque Assay Virion Virions Virus Replication United States > US Enquatrovirus N4-like podovirus bacteriophage Gamaleyavirus Klebsiella pneumoniae
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Summary:	is a common pathogen, associated with a wide spectrum of infections, and clinical isolates of often possess multiple antibiotic resistances. Here, we describe a novel lytic N4-like bacteriophage KP8, specific to , including its genome, partial structural proteome, biological properties, and proposed taxonomy. Electron microscopy revealed that KP8 belongs to the Podoviridae family. The size of the KP8 genome was 73,679 bp, and it comprised 97 putative open reading frames. Comparative genome analysis revealed that the KP8 genome possessed the highest similarity to the genomes of and phages, which are N4-like podoviruses. In addition, the KP8 genome showed gene synteny typical of the N4-like podoviruses and contained the gene encoding a large virion-encapsulated RNA polymerase. Phylogenetic analysis of the KP8 genome revealed that the KP8 genome formed a distinct branch within the clade, which included the members of and genera besides KP8. The average evolutionary divergences KP8/ and KP8/ were 0.466 and 0.447 substitutions per site (substitutes/site), respectively, similar to that between and genera (0.468 substitutes/site). The obtained data suggested that phage KP8 differs from other similar phages and may represent a new genus within the N4-like phages.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1999-4915 1999-4915
DOI:	10.3390/v11121115