Characterization of Ciprofloxacin-Resistant and Ciprofloxacin-Susceptible Uropathogenic Escherichia coli Obtained from Patients with Gynecological Cancer

The objective of this work was to assess the genetic characteristics of uropathogenic Escherichia coli, ciprofloxacin resistance or susceptibility, obtained from patients with gynecological cancer and urinary tract infection (UTI). Seventy-seven E. coli ciprofloxacin-resistant isolates and 38 ciprof...

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Bibliographic Details
Published in:	Current microbiology Vol. 73; no. 5; pp. 624 - 632
Main Authors:	Capett, Muniqui S., Vollú-Silva, Patricia, Melchiades, Vanessa A., Bokehi, Luciana C., Araújo, Fernanda M., Martins, Ianick Souto, Neves, Felipe P. G., Gonzalez, Alice G. M., Oswald, Eric, de Paula, Geraldo R., Teixeira, Lenise A.
Format:	Journal Article
Language:	English
Published:	New York Springer US 01-11-2016 Springer Nature B.V
Subjects:	Anti-Bacterial Agents - pharmacology Biomedical and Life Sciences Biotechnology Cancer Ciprofloxacin - pharmacology Deoxyribonucleic acid DNA Drug resistance Drug Resistance, Bacterial E coli Escherichia coli Escherichia coli Infections - microbiology Escherichia coli Proteins - genetics Female Genetic diversity Genital Neoplasms, Female - complications Humans Life Sciences Microbial Sensitivity Tests Microbiology Pathogens Phylogenetics Phylogeny Statistical analysis Toxins Urinary tract infections Urinary Tract Infections - etiology Urinary Tract Infections - microbiology Uropathogenic Escherichia coli - classification Uropathogenic Escherichia coli - drug effects Uropathogenic Escherichia coli - genetics Uropathogenic Escherichia coli - isolation & purification Gynecological Cancer Susceptible Isolate Virulence Factor Gene Fluoroquinolone Resistance Phylogenetic Group
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Summary:	The objective of this work was to assess the genetic characteristics of uropathogenic Escherichia coli, ciprofloxacin resistance or susceptibility, obtained from patients with gynecological cancer and urinary tract infection (UTI). Seventy-seven E. coli ciprofloxacin-resistant isolates and 38 ciprofloxacin-susceptible were analyzed by polymerase chain reaction (PCR) to determine the phylogenetic groups, virulence factors as iuc C, fyu A, hly C, cnf 1 genes, and pks pathogenicity island. The presence of genes related to ciprofloxacin resistance such as qnr A, qnr B, qnr S, aac ( 6 ′)- Ib - cr , and qep A, and the sequencing of DNA gyrase genes and topoisomerase IV were determined. The genetic profile of the isolates was determined by pulsed-field gel electrophoresis (PFGE). Statistical analysis was performed using Fisher’s exact test and Chi-square test. Phylogenetic group B2 was the most prevalent although a great genetic diversity was observed by PFGE. Only genes associated to siderophores were found in ciprofloxacin-resistant isolates; however, in ciprofloxacin-susceptible isolates, genes related to siderophores and toxin, were detected. Additionally qnrB was detected in both populations, ciprofloxacin resistant and susceptible. DNA mutations in gyr A were Ser-83-Leu and Asp-87-Asn and in par C were Ser-80-Ile and Glu-84-Val, Glu-84-Lys. In conclusion, it was observed a high prevalence of qnr B in the population studied; in addition, it was the first time the pks island was observed only in ciprofloxacin-susceptible isolates.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0343-8651 1432-0991
DOI:	10.1007/s00284-016-1104-y