3D In vitro model of the re‐epithelialization phase in the wound‐healing process

3D In vitro model of the re‐epithelialization phase in the wound‐healing process

Keratinocytes of the basal layer function are to maintain tissue homoeostasis and to fulfil skin repair in response to an external aggression. In wound‐healing, during re‐epithelialization phase, epithelial precursor cells gradually migrate from the edges of the wound. The epidermal reconstruction m...

Full description

Saved in:
Bibliographic Details
Published in:Experimental dermatology Vol. 27; no. 5; pp. 460 - 462
Main Authors: Deshayes, Nathalie, Bloas, Fabienne, Boissout, Florian, Lecardonnel, Jennifer, Paris, Maryline
Format: Journal Article
Language:English
Published: Denmark Wiley Subscription Services, Inc 01-05-2018
Subjects:
3D model
epidermal regeneration
Female
Humans
In Vitro Techniques
Keratinocytes
Keratinocytes - physiology
Models, Biological
Re-Epithelialization
Skin
Wound healing
3D model
epidermal regeneration
re-epithelialization
keratinocytes
wound-healing
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Keratinocytes of the basal layer function are to maintain tissue homoeostasis and to fulfil skin repair in response to an external aggression. In wound‐healing, during re‐epithelialization phase, epithelial precursor cells gradually migrate from the edges of the wound. The epidermal reconstruction model called standard model allows the vertical skin regeneration process (proliferation/differentiation) to being investigated, and keratinocyte function in preserving skin homoeostasis to being assessed. Here, we developed and characterized a 3D migration model, which introduces a step of keratinocytes migration such as the one observed in the phase of re‐epithelialization in wound‐healing process. We validated the added value and the discriminative potential of this model by demonstrating pro‐epithelializing effects of compounds. This new model allows the role of keratinocytes in different biomechanical and environmental requests to being better understood, and brings a new tool for compound screening and the study of mechanisms involved in skin regeneration.
Bibliography:SourceType-Other Sources-1
ObjectType-Article-2
content type line 63
ObjectType-Correspondence-1
ISSN:0906-6705
1600-0625
DOI:10.1111/exd.13390