Biochemical analyses of the cement float of the goose barnacle Dosima fascicularis - a preliminary study

Biochemical analyses of the cement float of the goose barnacle Dosima fascicularis - a preliminary study

The goose barnacle Dosima fascicularis produces an excessive amount of adhesive (cement), which has a double function, being used for attachment to various substrata and also as a float (buoy). This paper focuses on the chemical composition of the cement, which has a water content of 92%. Scanning e...

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Bibliographic Details
Published in:Biofouling (Chur, Switzerland) Vol. 30; no. 8; pp. 949 - 963
Main Authors: Zheden, Vanessa, Klepal, Waltraud, von Byern, Janek, Bogner, Fabian Robert, Thiel, Karsten, Kowalik, Thomas, Grunwald, Ingo
Format: Journal Article
Language:English
Published: England Taylor & Francis 14-09-2014
Subjects:
1D/2D PAGE
Adhesives
Animals
Arthropod Proteins - analysis
Arthropod Proteins - chemistry
barnacle adhesive/cement
carbohydrate
Denmark
FTIR/Raman spectroscopy
hydrogel
Microscopy, Electron, Scanning
protein primary/secondary structure
Spectrum Analysis
Thoracica - chemistry
Denmark
protein primary/secondary structure
hydrogel
1D/2D PAGE
FTIR/Raman spectroscopy
carbohydrate
barnacle adhesive/cement
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Summary:The goose barnacle Dosima fascicularis produces an excessive amount of adhesive (cement), which has a double function, being used for attachment to various substrata and also as a float (buoy). This paper focuses on the chemical composition of the cement, which has a water content of 92%. Scanning electron microscopy with EDX was used to measure the organic elements C, O and N in the foam-like cement. Vibrational spectroscopy (FTIR, Raman) provided further information about the overall secondary structure, which tended towards a β-sheet. Disulphide bonds could not be detected by Raman spectroscopy. The cystine, methionine, histidine and tryptophan contents were each below 1% in the cement. Analyses of the cement revealed a protein content of 84% and a total carbohydrate content of 1.5% in the dry cement. The amino acid composition, 1D/2D-PAGE and MS/MS sequence analysis revealed a de novo set of peptides/proteins with low homologies with other proteins such as the barnacle cement proteins, largely with an acidic pI between 3.5 and 6.0. The biochemical composition of the cement of D. fascicularis is similar to that of other barnacles, but it shows interesting variations.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0892-7014
1029-2454
DOI:10.1080/08927014.2014.954557