Oncogenic Viruses in Skull Base Chordomas

Oncogenic Viruses in Skull Base Chordomas

Chordomas are rare tumors assumed to derive from notochordal remnants. We believe that a molecular switch is responsible for their malignant behavior. The involvement of oncogenic viruses has not been studied, however. Thus, in the present study, we investigated the presence of oncogenic viruses in...

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Published in:World neurosurgery Vol. 112; pp. e7 - e13
Main Authors: Yakkioui, Youssef, Speel, Ernst-Jan M., Van Overbeeke, Jacobus J., Boderie, Merel J.M., Pujari, Sreedhar, Hausen, Axel Zur, Wolffs, Petra F.G., Temel, Yasin
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01-04-2018
Subjects:
Cancer
Chondrosarcoma
Chordoma
Oncogenic virus
PVB19
Virus
Chondrosarcoma
HPyV
TMA
HSV
PVB19
MCPyV
CMV
HPV
Virus
Chordoma
Oncogenic virus
KSHV
HHV
EBV
Cancer
ISH
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Summary:Chordomas are rare tumors assumed to derive from notochordal remnants. We believe that a molecular switch is responsible for their malignant behavior. The involvement of oncogenic viruses has not been studied, however. Thus, in the present study, we investigated the presence of oncogenic viruses in chordomas. DNA and RNA from snap-frozen chordoma (n = 18) and chondrosarcoma (n = 15) specimens were isolated. Real-time PCR or RT-PCR was performed to assess the presence of multiple oncogenic viruses, including herpesviridea (herpes simplex virus [HSV]-1, HSV-2, Epstein-Barr virus [EBV], cytomegalovirus, human herpesvirus [HHV]- 6, HHV-7, and Kaposi's sarcoma–associated herpesvirus), polyomaviridea (parvovirus B19 [PVB19], BK virus, JC virus, Simian virus 40, Merkel cell polyomavirus, human polyomavirus [HPyV]-6, and HPyV-7), papillomaviridae, and respiratory viruses. Immunohistochemistry (IHC) and in situ hybridization (ISH) were used to validate the positive results. PVB19 DNA was detected in 4 of 18 chordomas (22%) and in 1 of 15 chondrosarcomas (7%). IHC recognizing the VP2 capsid protein of PVB19 showed a positive cytoplasmic staining in 44% of the cases (14 of 32). HHV7 DNA was present in 6 of the 18 chordomas (33%). Genomic DNA of EBV was found in 22% of the samples; however, no positive results were found on ISH. None of the chordoma cases showed any presence of DNA from the remaining viruses. Viral involvement in the etiology of chordomas is likely, with PVB19 the most distinguishing.
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ISSN:1878-8750
1878-8769
DOI:10.1016/j.wneu.2017.11.117