Gain of chromosome arm 9p is characteristic of primary mediastinal b-cell lymphoma (MBL): Comprehensive molecular cytogenetic analysis and presentation of a novel MBL cell line

Primary mediastinal B‐cell lymphoma (MBL) is an aggressive Non‐Hodgkin's Lymphoma, which has been recognized as a distinct disease entity. We performed a comprehensive molecular cytogenetic study analyzing 43 MBLs. By comparative genomic hybridization (CGH), the most common aberrations were gai...

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Published in:	Genes chromosomes & cancer Vol. 30; no. 4; pp. 393 - 401
Main Authors:	Bentz, Martin, Barth, Thomas F. E., Brüderlein, Silke, Bock, Daliah, Schwerer, Michael J., Baudis, Michael, Joos, Stefan, Viardot, Andreas, Feller, Alfred C., Müller-Hermelink, Hans-Konrad, Lichter, Peter, Döhner, Hartmut, Möller, Peter
Format:	Journal Article
Language:	English
Published:	New York John Wiley & Sons, Inc 01-04-2001
Subjects:	Adolescent Adult Aged Chromosome 9 Chromosome Aberrations - genetics Chromosomes, Human, Pair 9 - genetics Cytogenetic Analysis Female Humans In Situ Hybridization, Fluorescence Karyotyping Lymphoma, B-Cell - genetics Lymphoma, B-Cell - pathology Male Mediastinal Neoplasms - genetics Mediastinal Neoplasms - pathology Middle Aged Non-Hodgkin's lymphoma Nucleic Acid Hybridization - methods Primary mediastinal B-cell lymphoma Tumor Cells, Cultured - pathology
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Summary:	Primary mediastinal B‐cell lymphoma (MBL) is an aggressive Non‐Hodgkin's Lymphoma, which has been recognized as a distinct disease entity. We performed a comprehensive molecular cytogenetic study analyzing 43 MBLs. By comparative genomic hybridization (CGH), the most common aberrations were gains of chromosome arms 9p and Xq, which were present in 56% and 40% of cases, respectively. Based on the limited resolution of CGH, this technique may underestimate the real incidence of aberrations. Therefore, we also did an interphase cytogenetic study with eight DNA probes mapping to chromosome regions frequently altered in B‐cell lymphomas. With this approach, both 9p and Xq gains were found in more than 70% of cases (75% and 87%, respectively). The findings were compared with results obtained in 308 other B‐cell lymphomas. Gains in 9p were identified in only six of the 308 cases, and only one of these lymphomas with 9p gains was not primarily extranodal in origin (P < 10−20 for CGH data and P < 10−11 for fluorescence in situ hybridization data). We also present a novel MBL cell line, MedB‐1, which carries the genetic aberrations characteristic of this entity. © 2001 Wiley‐Liss, Inc.
Bibliography:	ArticleID:GCC1105 Deutsche Krebshilfe - No. M 47/95 Be I; No. 70-2310-Ba I Deutsche Forschungsgemeinschaft - No. Be 1454/5-2 Medical Faculty of the University of Ulm - No. P389 ark:/67375/WNG-6LXKX7H3-W istex:8D71A0C053E15AD4DD2F9786515DF90D5884F56C ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1045-2257 1098-2264
DOI:	10.1002/1098-2264(2001)9999:9999<::AID-GCC1105>3.0.CO;2-I