Uncoupling effect of fatty acids on heart muscle mitochondria and submitochondrial particles

The effect of ATP/ADP-antiporter inhibitors on palmitate-induced uncoupling was studied in heart muscle mitochondria and inside-out submitochondrial particles. In both systems palmitate is found to decrease the respiration-generated membrane potential. In mitochondria, this effect is specifically ab...

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Bibliographic Details
Published in:	FEBS letters Vol. 295; no. 1; pp. 51 - 54
Main Authors:	Dedukhova, V.I., Mokhova, E.N., Skulachev, V.P., Starkov, A.A., Arrigoni-Martelli, E., Bobyleva, V.A.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 16-12-1991 Elsevier
Subjects:	2,4-dinitrophenol Animals ATP/ADP-antiporter Atr atractylate Atractyloside - analogs & derivatives Atractyloside - pharmacology Biological and medical sciences Bongkrekic Acid - pharmacology bovine serum albumin BSA Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology carboxyatractylate CAtr Cell physiology DNP EDTA effects on EGTA ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid ethylenediamine-N,N,N′N′-tetraacetic acid Fatty acid fatty acids FCCP Fundamental and applied biological sciences. Psychology heart Heart mitochondria Kinetics Membrane and intracellular transports mitochondria Mitochondria, Heart - drug effects Mitochondria, Heart - metabolism Mitochondrial ADP, ATP Translocases - antagonists & inhibitors Molecular and cellular biology MOPS morpholinopropane sulphonate muscles Oxygen Consumption - drug effects p-trifluoromethoxycarbonylcyanide phenylhydrazone Palmitic Acid Palmitic Acids - pharmacology PCB phenyldicarbaundecaborane Rabbits SDS sodium dodecyl sulfate Sodium Dodecyl Sulfate - pharmacology Submitochondrial particle Submitochondrial Particles - drug effects Submitochondrial Particles - metabolism tetraphenyl phosphonium TPP transmembrane electric potential difference Uncoupling Uncoupling Agents - pharmacology Uncoupling MOPS Submitochondrial particle CAtr SDS DNP FCCP EDTA PCB EGTA BSA Δψ Heart mitochondria TPP Fatty acid ATP/ADP-antiporter Atr Heart Vertebrata Mitochondria Mammalia Rabbit Coupled transport Lagomorpha ADP ATP Fatty acids
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Summary:	The effect of ATP/ADP-antiporter inhibitors on palmitate-induced uncoupling was studied in heart muscle mitochondria and inside-out submitochondrial particles. In both systems palmitate is found to decrease the respiration-generated membrane potential. In mitochondria, this effect is specifically abolished by carboxyatractylate (CAtr) a non-penetrating inhibitor of antiporter. In submitochondrial particles, CAtr does not abolish the palmitate-induced potential decrease. At the same time, bongkrekic acid, a penetrating inhibitor of the antiporter, suppresses the palmitate effect on the potential both in mitochondria and particles. Palmitoyl-CoA which is known to inhibit the antiporter in mitochondria as well as in particles decreases the palmitate uncoupling efficiency in both these systems. These data are in agreement with the hypothesis that the ATP/ADP-antiporter is involved in the action of free fatty acids as natural uncouplers of oxidative phosphorylation.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(91)81382-I