A Comparative Kidney Transcriptome Analysis of Bicarbonate-Loaded insrr -Null Mice

The maintenance of plasma pH is critical for life in all organisms. The kidney plays a critical role in acid-base regulation in vertebrates by controlling the plasma concentration of bicarbonate. The receptor tyrosine kinase IRR (insulin receptor-related receptor) is expressed in renal β-intercalate...

Full description

Saved in:

Bibliographic Details
Published in:	Current issues in molecular biology Vol. 45; no. 12; pp. 9709 - 9722
Main Authors:	Gantsova, E A, Serova, O V, Eladari, D, Bobrovskiy, D M, Petrenko, A G, Elchaninov, A V, Deyev, I E
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 04-12-2023
Subjects:	alkaline pH alkalosis intercalated cells IRR kidney receptor tyrosine kinases kidney receptor tyrosine kinases intercalated cells acid–base balance IRR alkaline pH alkalosis
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The maintenance of plasma pH is critical for life in all organisms. The kidney plays a critical role in acid-base regulation in vertebrates by controlling the plasma concentration of bicarbonate. The receptor tyrosine kinase IRR (insulin receptor-related receptor) is expressed in renal β-intercalated cells and is involved in alkali sensing due to its ability to autophosphorylate under alkalization of extracellular medium (pH > 7.9). In mice with a knockout of the gene, which encodes for IRR, urinary bicarbonate secretion in response to alkali loading is impaired. The specific regulatory mechanisms in the kidney that are under the control of IRR remain unknown. To address this issue, we analyzed and compared the kidney transcriptomes of wild-type and knockout mice under basal or bicarbonate-loaded conditions. Transcriptomic analyses revealed a differential regulation of a number of genes in the kidney. Using TaqMan real-time PCR, we confirmed different expressions of the , , , , , , , , and genes in IRR knockout mice. Also, we found that the expression of the gene is increased in wild-type mice after bicarbonate loading but not in knockout mice. Gene set enrichment analysis between the IRR knockout and wild-type samples identified that knockout causes alterations in expression of genes related mostly to the ATP metabolic and electron transport chain processes.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1467-3045 1467-3037 1467-3045
DOI:	10.3390/cimb45120606