Search Results - "Bobik, T V"

An ELISA Platform for the Quantitative Analysis of SARS-CoV-2 RBD-neutralizing Antibodies As an Alternative to Monitoring of the Virus-Neutralizing Activity by Kostin, N N, Bobik, T V, Skryabin, G A, Simonova, M A, Knorre, V D, Abrikosova, V A, Mokrushina, Y A, Smirnov, I V, Aleshenko, N L, Kruglova, N A, Mazurov, D V, Nikitin, A E, Gabibov, A G

“…Monitoring of the level of the virus-neutralizing activity of serum immunoglobulins ensures that one can reliably assess the effectiveness of any protection…”
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Cloning and characterization of serpin from red king crab Paralithodes camtschaticus by Kostin, N.N., Bobik, T.V., Shurdova, E.M., Ziganshin, R.H., Surina, E.A., Shagin, D.A., Shagina, I.A., Knorre, V.D., Isaev, V.A., Rudenskaya, G.N., Gabibov, A.G., Smirnov, I.V.

“…Serpins are a family of serine protease inhibitors that are involved in numerous physiological processes and are known to regulate innate immunity pathways. To…”
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Immunoliposomes As a Promising Antiviral Agent against SARS-CoV-2 by Bobik, T. V., Simonova, M. A., Rushkevich, N. U., Kostin, N. N., Skryabin, G. A., Knorre, V. D., Schulga, A. A., Konovalova, E. V., Proshkina, G. M., Gabibov, A. G., Deev, S. M.

“…According to the World Health Organization, as of January 3, 2020 to September 13, 2023, there were approximately 23 million confirmed cases of COVID-19…”
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Expression of catalytic antibodies in eukaryotic systems by Zakharov, A. V, Smirnov, I. V, Serebryakova, M. V, Dronina, M. A, Kaznacheeva, A. V, Kurkova, I. N, Belogurov, A. A, Friboulet, A, Ponomarenko, N. A, Gabibov, A. G, Bobik, T. V

“…Expression of recombinant antibodies in mammalian cells is one of the key problems in immuno-biotechnology. Alternatively, expression of a broad panel of…”
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Development of a Serum-Free Media Based on the Optimal Combination of Recombinant Protein Additives and Hydrolysates of Non-animal Origin to Produce Immunoglobulins by Balabashin, D. S., Kaliberda, E. N., Smirnov, I. V., Mokrushina, Y. A., Bobik, T. V., Aliev, T. K., Dolgikh, D. A., Kirpichnikov, M. P.

“…The potential ability to cultivate a recombinant immunoglobulin (IgG1)-producer line in Iscove’s Modified Dulbecco Medium (IMDM) as the base cultivation medium…”
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Analysis of the Specificity of Auto-Reactive Antibodies to Individual Fragments of the Extracellular Domain of Desmoglein 3 in Patients with Pemphigus Vulgaris by Kubanov, A. A., Deryabin, D. G., Shpilevaya, M. V., Karamova, A. E., Nikonorov, A. A., Larina, E. N., Aliev, T. K., Dolgikh, D. A., Bobik, T. V., Smirnov, I. V., Gabibov, A. G., Kirpichnikov, M. P.

“…A method for the analysis of the epitope specificity of auto-reactive antibodies to desmoglein 3 (Dsg3) using competitive ELISA has been developed. It is based…”
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B Cell Profiling in Patients with Pemphigus Vulgaris by Abrikosova, V A, Mokrushina, Y A, Ovchinnikova, L A, Larina, E N, Terekhov, S S, Baranova, M N, Lomakin, Y A, Balabashin, D S, Bobik, T V, Kaliberda, E N, Knorre, V D, Shpilevaya, M V, Aliev, T K, Deryabin, D G, Karamova, A E, Kubanov, A A, Kirpichnikov, M P, Smirnov, I V

“…Pemphigus vulgaris is a severe, socially significant autoimmune disease associated with autoantibodies to the desmoglein 3 antigen. The disease affects all age…”
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COVID-19 in Russia: Clinical and Immunological Features of the First-Wave Patients by Bobik, T V, Kostin, N N, Skryabin, G A, Tsabai, P N, Simonova, M A, Knorre, V D, Stratienko, O N, Aleshenko, N L, Vorobiev, I I, Khurs, E N, Mokrushina, Yu A, Smirnov, I V, Alekhin, A I, Nikitin, A E, Gabibov, A G

“…The coronavirus disease outbreak in 2019 (COVID-19) has now achieved the level of a global pandemic and affected more than 100 million people on all five…”
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Production of Recombinant Human Transferrin in Eukaryotic Pichia pastoris Expression System by Bobik, T. V., Popov, R. Yu, Aliev, T. K., Mokrushina, Yu. A., Shamborant, O. G., Khurs, E. N., Knorre, V. D., Smirnov, I. V., Gabibov, A. G.

“…The development and manufacturing of serum-free culture media allowing reducing the costs of preparations and standardizing the biotechnological process are…”
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Recombinant Fragment of the Extracellular Domain of Human Desmoglein 3 Fused with the Fc-Fragment of Human IgG1 Selectively Adsorbs Autoreactive Antibodies from the Sera of Pemphigus Patients by Larina, E. N., Karasev, V. S., Shpilevaya, M. V., Aliev, T. K., Bochkova, O. P., Karamova, A. E., Balabashin, D. S., Deryabin, D. G., Bobik, T. V., Smirnov, I. V., Kubanov, A. A., Staroverov, S. M., Gabibov, A. G., Kirpichnikov, M. P.

“…Using the recombinant second fragment of the extracellular domain (EC2) of human desmoglein type 3 (Dsg3) as an affinity ligand, an immunosorbent was obtained…”
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Generation of Highly Specific Proteolytic Biocatalysts by Screening Technologies by Bobik, T. V., Kostin, N. N., Knorre, V. D., Gabibov, A. G., Smirnov, I. V.

“…We propose a yeast display-based system for screening of proteolytic enzyme libraries that utilizes substrate protein adsorbed on the yeast cell surface and…”
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Directed Change in TNFα Specificity to Create DR5 Antagonists by Ukrainskaya, V. M., Bobik, T. V., Argentova-Stevens, A., Slutskaya, E. A., Kalinin, R. S., Gabibov, A. G., Stepanov, A. V.

“…Death receptor 5 (DR5) is a promising target for antitumor therapy due to its high expression on different tumor cells. Resistance of various tumor cells…”
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Preparation of Recombinant Serpin from Red King Crab Paralithodes сamtschaticus for Biomedical Research Purposes by Kostin, N. N., Ponomarenko, N. A., Isaev, V. A., Rudenskaya, G. N., Bobik, T. V., Gabibov, A. G., Smirnov, I. V.

“…Genetic constructs with different leader sequences for intra- and extracellular expression of the target protein were generated and an original method for…”
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Genetic Engineering of Native Chain Combinations of B-Cell Repertoires on the Surface of Methylotrophic Yeasts Pichia pastoris by Bobik, T. V., Shurdova, E. M., Smirnov, I. V., Ponomarenko, N. A., Khurs, E. N., Knorre, V. D., Gabibov, A. G.

“…We designed genetic constructs for exposing Fab-fragment library of natively paired single cell B-cell receptors on the surface of Pichia pastoris yeast cells…”
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Anti-DNA autoantibodies reveal toxicity to tumor cell lines by Kozyr, A.V, Sashchenko, L.P, Kolesnikov, A.V, Zelenova, N.A, Khaidukov, S.V, Ignatova, A.N, Bobik, T.V, Gabibov, A.G, Alekberova, Z.S, Suchkov, S.V, Gnuchev, N.V

“…Cytotoxicity of anti-DNA autoantibodies from sera of SLE and CLL patients was assayed on permanent cell lines L929, HL-60, Raji, and K562. L929 cells appeared…”
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Analysis of Immunogenicity of Intracellular CTAR Fragments of Epstein—Barr Virus Latent Phase Protein LMP1 by Lomakin, Ya. A., Shmidt, A. A., Bobik, T. V., Chernov, A. S., Pyrkov, A. Yu, Aleksandrova, N. M., Okunola, D. O., Vaskina, M. I., Ponomarenko, N. A., Telegin, G. B., Dubina, M. V., Belogurov, A. A.

“…Intracellular fragments of latent phase protein LMP1 of Epstein—Barr virus, denoted as CTAR1/2/3, can trigger a variety of cell cascades and contribute to the…”
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Expression of DNA-Encoded Antidote to Organophosphorus Toxins in the Methylotrophic Yeast Pichia Pastoris by Terekhov, S. S., Bobik, T. V., Mokrushina, Yu. A., Stepanova, A. V., Aleksandrova, N. M., Smirnov, I. V., Belogurov, A. A., Ponomarenko, N. A., Gabibov, A. G.

“…A platform for the cloning and expression of active human butyrylcholinesterase (BuChE) in the yeast Pichia рastoris is first presented. Genetic constructs for…”
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New Genetic Constructs for Generation of Stable Therapeutic Antibodies to Organophosphorus Toxins in Methylotrophic Yeasts Pichia Pastoris by Mokrushina, Yu. A., Stepanova, A. V., Bobik, T. V., Smirnov, I. V., Gabibov, A. G.

“…We propose a new method of obtaining of stable Fab-fragments of antibodies in Pichia pastoris expression system. Recently, we obtained Fab-fragments of…”
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Chemical Polysialylation and In Vivo Tetramerization Improve Pharmacokinetic Characteristics of Recombinant Human Butyrylcholinesterase-Based Bioscavengers by Terekhov, S S, Smirnov, I V, Shamborant, O G, Bobik, T V, Ilyushin, D G, Murashev, A N, Dyachenko, I A, Palikov, V A, Knorre, V D, Belogurov, A A, Ponomarenko, N A, Kuzina, E S, Genkin, D D, Masson, P, Gabibov, A G

“…Organophosphate toxins (OPs) are the most toxic low-molecular compounds. The extremely potent toxicity of OPs is determined by their specificity toward the…”
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Immunoproteasome enhances intracellular proteolysis of myelin basic protein by Kuzina, E. S., Chernolovskaya, E. L., Kudriaeva, A. A., Zenkova, M. A., Knorre, V. D., Surina, E. A., Ponomarenko, N. A., Bobik, T. V., Smirnov, I. V., Bacheva, A. V., Belogurov, A. A., Gabibov, A. G., Vlasov, V. V.

“…Proteasome is a multisubunit protein complex that exhibits proteolytic activity and is present in the nuclei and cytoplasm of cells. The 20S proteasome, which…”
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