Search Results - "Boal, Amie K"

Structural basis for halogenation by iron- and 2-oxo-glutarate-dependent enzyme WelO5 by Mitchell, Andrew J, Zhu, Qin, Maggiolo, Ailiena O, Ananth, Nikhil R, Hillwig, Matthew L, Liu, Xinyu, Boal, Amie K

“…WelO5 is an iron- and 2-oxoglutarate-dependent halogenase involved in welwitindolinone biosynthesis. A collection of X-ray crystal structures reveals the…”
Get full text

DNA-Mediated Charge Transport in Redox Sensing and Signaling by Genereux, Joseph C, Boal, Amie K, Barton, Jacqueline K

“…The transport of charge through the DNA base-pair stack offers a route to carry out redox chemistry at a distance. Here we describe characteristics of this…”
Get full text

A Peroxodiiron(III/III) Intermediate Mediating Both N‑Hydroxylation Steps in Biosynthesis of the N‑Nitrosourea Pharmacophore of Streptozotocin by the Multi-domain Metalloenzyme SznF by McBride, Molly J, Sil, Debangsu, Ng, Tai L, Crooke, Anne Marie, Kenney, Grace E, Tysoe, Christina R, Zhang, Bo, Balskus, Emily P, Boal, Amie K, Krebs, Carsten, Bollinger, J. Martin

“…The alkylating warhead of the pancreatic cancer drug strepto­zotocin (SZN) contains an N-nitroso­urea moiety constructed from N ω-methyl-l-arginine (l-NMA) by…”
Get full text

Structure of a Ferryl Mimic in the Archetypal Iron(II)- and 2‑(Oxo)-glutarate-Dependent Dioxygenase, TauD by Davis, Katherine M, Altmyer, Madison, Martinie, Ryan J, Schaperdoth, Irene, Krebs, Carsten, Bollinger, J. Martin, Boal, Amie K

“…Iron­(II)- and 2-(oxo)-glutarate-dependent (Fe/2OG) oxygenases catalyze a diverse array of oxidation reactions via a common iron­(IV)-oxo (ferryl)…”
Get full text

Structural Basis for Methyl Transfer by a Radical SAM Enzyme by Boal, Amie K., Grove, Tyler L., McLaughlin, Monica I., Yennawar, Neela H., Booker, Squire J., Rosenzweig, Amy C.

“…The radical S-adenosyl-L-methionine (SAM) enzymes RlmN and Cfr methylate 235 ribosomal RNA, modifying the C2 or C8 position of adenosine 2503. The methyl…”
Get full text

Substrate-Triggered Formation of a Peroxo-Fe2(III/III) Intermediate during Fatty Acid Decarboxylation by UndA by Zhang, Bo, Rajakovich, Lauren J, Van Cura, Devon, Blaesi, Elizabeth J, Mitchell, Andrew J, Tysoe, Christina R, Zhu, Xuejun, Streit, Bennett R, Rui, Zhe, Zhang, Wenjun, Boal, Amie K, Krebs, Carsten, Bollinger, J. Martin

“…The iron-dependent oxidase UndA cleaves one C3–H bond and the C1–C2 bond of dodecanoic acid to produce 1-undecene and CO2. A published X-ray crystal structure…”
Get full text

Steric Enforcement of cis-Epoxide Formation in the Radical C–O-Coupling Reaction by Which (S)‑2-Hydroxypropylphosphonate Epoxidase (HppE) Produces Fosfomycin by Zhou, Shengbin, Pan, Juan, Davis, Katherine M, Schaperdoth, Irene, Wang, Bo, Boal, Amie K, Krebs, Carsten, Bollinger, J. Martin

“…(S)-2-Hydroxypropylphosphonate [(S)-2-HPP, 1] epoxidase (HppE) reduces H2O2 at its nonheme-iron cofactor to install the oxirane “warhead” of the antibiotic…”
Get full text

Mechanism of the C5 Stereoinversion Reaction in the Biosynthesis of Carbapenem Antibiotics by Chang, Wei-chen, Guo, Yisong, Wang, Chen, Butch, Susan E., Rosenzweig, Amy C., Boal, Amie K., Krebs, Carsten, Bollinger, J. Martin

“…The bicyclic β-lactam/2-pyrrolidine precursor to all carbapenem antibiotics is biosynthesized by attachment of a carboxymethylene unit to C5 of L-proline…”
Get full text

A New Microbial Pathway for Organophosphonate Degradation Catalyzed by Two Previously Misannotated Non-Heme-Iron Oxygenases by Rajakovich, Lauren J, Pandelia, Maria-Eirini, Mitchell, Andrew J, Chang, Wei-chen, Zhang, Bo, Boal, Amie K, Krebs, Carsten, Bollinger, J. Martin

“…The assignment of biochemical functions to hypothetical proteins is challenged by functional diversification within many protein structural superfamilies. This…”
Get full text

Structural Basis for Superoxide Activation of Flavobacterium johnsoniae Class I Ribonucleotide Reductase and for Radical Initiation by Its Dimanganese Cofactor by Rose, Hannah R, Ghosh, Manas K, Maggiolo, Ailiena O, Pollock, Christopher J, Blaesi, Elizabeth J, Hajj, Viviane, Wei, Yifeng, Rajakovich, Lauren J, Chang, Wei-chen, Han, Yilin, Hajj, Mariana, Krebs, Carsten, Silakov, Alexey, Pandelia, Maria-Eirini, Bollinger, J. Martin, Boal, Amie K

“…A ribonucleotide reductase (RNR) from Flavobacterium johnsoniae (Fj) differs fundamentally from known (subclass a–c) class I RNRs, warranting its assignment to…”
Get full text

Rerouting the Pathway for the Biosynthesis of the Side Ring System of Nosiheptide: The Roles of NosI, NosJ, and NosK by Badding, Edward D, Grove, Tyler L, Gadsby, Lauren K, LaMattina, Joseph W, Boal, Amie K, Booker, Squire J

“…Nosiheptide (NOS) is a highly modified thiopeptide antibiotic that displays formidable in vitro activity against a variety of Gram-positive bacteria. In…”
Get full text

Structural Basis for Activation of Class Ib Ribonucleotide Reductase by Boal, Amie K, Cotruvo, Joseph A. Jr, Stubbe, JoAnne, Rosenzweig, Amy C

“…The class Ib ribonucleotide reductase of Escherichia coli can initiate reduction of nucleotides to deoxynucleotides with either a MnIII₂-tyrosyl radical (Y )…”
Get full text

Redox signaling between DNA repair proteins for efficient lesion detection by Boal, Amie K, Genereux, Joseph C, Sontz, Pamela A, Gralnick, Jeffrey A, Newman, Dianne K, Barton, Jacqueline K

“…Base excision repair (BER) enzymes maintain the integrity of the genome, and in humans, BER mutations are associated with cancer. Given the remarkable…”
Get full text

Structural basis for an unprecedented enzymatic alkylation in cylindrocyclophane biosynthesis by Braffman, Nathaniel R, Ruskoski, Terry B, Davis, Katherine M, Glasser, Nathaniel R, Johnson, Cassidy, Okafor, C Denise, Boal, Amie K, Balskus, Emily P

“…The cyanobacterial enzyme CylK assembles the cylindrocyclophane natural products by performing two unusual alkylation reactions, forming new carbon-carbon…”
Get full text

Organophosphonate-degrading PhnZ reveals an emerging family of HD domain mixed-valent diiron oxygenases by Wörsdörfer, Bigna, Lingaraju, Mahesh, Yennawar, Neela H., Boal, Amie K., Krebs, Carsten, Bollinger, J. Martin, Pandelia, Maria-Eirini

“…The founding members of the HD-domain protein superfamily are phosphohydrolases, and newly discovered members are generally annotated as such. However, myo…”
Get full text

Biological contexts for DNA charge transport chemistry by Merino, Edward J, Boal, Amie K, Barton, Jacqueline K

“…Many experiments have now shown that double helical DNA can serve as a conduit for efficient charge transport (CT) reactions over long distances in vitro…”
Get full text

Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers by Fuller, Franklin D, Gul, Sheraz, Chatterjee, Ruchira, Burgie, E Sethe, Young, Iris D, Lebrette, Hugo, Srinivas, Vivek, Brewster, Aaron S, Michels-Clark, Tara, Clinger, Jonathan A, Andi, Babak, Ibrahim, Mohamed, Pastor, Ernest, de Lichtenberg, Casper, Hussein, Rana, Pollock, Christopher J, Zhang, Miao, Stan, Claudiu A, Kroll, Thomas, Fransson, Thomas, Weninger, Clemens, Kubin, Markus, Aller, Pierre, Lassalle, Louise, Bräuer, Philipp, Miller, Mitchell D, Amin, Muhamed, Koroidov, Sergey, Roessler, Christian G, Allaire, Marc, Sierra, Raymond G, Docker, Peter T, Glownia, James M, Nelson, Silke, Koglin, Jason E, Zhu, Diling, Chollet, Matthieu, Song, Sanghoon, Lemke, Henrik, Liang, Mengning, Sokaras, Dimosthenis, Alonso-Mori, Roberto, Zouni, Athina, Messinger, Johannes, Bergmann, Uwe, Boal, Amie K, Bollinger, J Martin, Krebs, Carsten, Högbom, Martin, Phillips, George N, Vierstra, Richard D, Sauter, Nicholas K, Orville, Allen M, Kern, Jan, Yachandra, Vittal K, Yano, Junko

Get full text

Assembly of the unusual oxacycles in the orthosomycin antibiotics by Boal, Amie K., Bollinger, J. Martin, Chang, Wei-chen

Get full text

The Dimanganese(II) Site of Bacillus subtilis Class Ib Ribonucleotide Reductase by Boal, Amie K, Cotruvo, Joseph A, Stubbe, JoAnne, Rosenzweig, Amy C

“…Class Ib ribonucleotide reductases (RNRs) use a dimanganese-tyrosyl radical cofactor, MnIII 2-Y•, in their homodimeric NrdF (β2) subunit to initiate reduction…”
Get full text

DNA-Bound Redox Activity of DNA Repair Glycosylases Containing [4Fe-4S] Clusters by Boal, Amie K, Yavin, Eylon, Lukianova, Olga A, O'Shea, Valerie L, David, Sheila S, Barton, Jacqueline K

“…MutY and endonuclease III, two DNA glycosylases from Escherichia coli, and AfUDG, a uracil DNA glycosylase from Archeoglobus fulgidus, are all base excision…”
Get full text