Search Results - "Bloom, Jesse D"

Permissive Secondary Mutations Enable the Evolution of Influenza Oseltamivir Resistance by Bloom, Jesse D, Gong, Lizhi Ian, Baltimore, David

“…The His²⁷⁴[rightward arrow]Tyr²⁷⁴ (H274Y) mutation confers oseltamivir resistance on N1 influenza neuraminidase but had long been thought to compromise viral…”
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A human coronavirus evolves antigenically to escape antibody immunity by Eguia, Rachel T, Crawford, Katharine H D, Stevens-Ayers, Terry, Kelnhofer-Millevolte, Laurel, Greninger, Alexander L, Englund, Janet A, Boeckh, Michael J, Bloom, Jesse D

“…There is intense interest in antibody immunity to coronaviruses. However, it is unknown if coronaviruses evolve to escape such immunity, and if so, how…”
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How single mutations affect viral escape from broad and narrow antibodies to H1 influenza hemagglutinin by Doud, Michael B., Lee, Juhye M., Bloom, Jesse D.

“…Influenza virus can escape most antibodies with single mutations. However, rare antibodies broadly neutralize many viral strains. It is unclear how easily…”
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Complete map of SARS-CoV-2 RBD mutations that escape the monoclonal antibody LY-CoV555 and its cocktail with LY-CoV016 by Starr, Tyler N., Greaney, Allison J., Dingens, Adam S., Bloom, Jesse D.

“…Monoclonal antibodies and antibody cocktails are a promising therapeutic and prophylaxis for coronavirus disease 2019 (COVID-19). However, ongoing evolution of…”
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Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding by Starr, Tyler N., Greaney, Allison J., Hilton, Sarah K., Ellis, Daniel, Crawford, Katharine H.D., Dingens, Adam S., Navarro, Mary Jane, Bowen, John E., Tortorici, M. Alejandra, Walls, Alexandra C., King, Neil P., Veesler, David, Bloom, Jesse D.

“…The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein mediates viral attachment to ACE2 receptor and is a major determinant of host range and…”
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Extreme heterogeneity of influenza virus infection in single cells by Russell, Alistair B, Trapnell, Cole, Bloom, Jesse D

“…Viral infection can dramatically alter a cell's transcriptome. However, these changes have mostly been studied by bulk measurements on many cells. Here we use…”
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The inherent mutational tolerance and antigenic evolvability of influenza hemagglutinin by Thyagarajan, Bargavi, Bloom, Jesse D

“…Influenza is notable for its evolutionary capacity to escape immunity targeting the viral hemagglutinin. We used deep mutational scanning to examine the extent…”
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In the light of directed evolution: Pathways of adaptive protein evolution by Bloom, Jesse D, Arnold, Frances H

“…Directed evolution is a widely-used engineering strategy for improving the stabilities or biochemical functions of proteins by repeated rounds of mutation and…”
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Antibodies elicited by mRNA-1273 vaccination bind more broadly to the receptor binding domain than do those from SARS-CoV-2 infection by Greaney, Allison J, Loes, Andrea N, Gentles, Lauren E, Crawford, Katharine H D, Starr, Tyler N, Malone, Keara D, Chu, Helen Y, Bloom, Jesse D

“…The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with mutations in key antibody epitopes has raised concerns that…”
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Dynamics of Neutralizing Antibody Titers in the Months After Severe Acute Respiratory Syndrome Coronavirus 2 Infection by Crawford, Katharine H D, Dingens, Adam S, Eguia, Rachel, Wolf, Caitlin R, Wilcox, Naomi, Logue, Jennifer K, Shuey, Kiel, Casto, Amanda M, Fiala, Brooke, Wrenn, Samuel, Pettie, Deleah, King, Neil P, Greninger, Alexander L, Chu, Helen Y, Bloom, Jesse D

“…Abstract Most individuals infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) develop neutralizing antibodies that target the viral…”
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Deep mutational scans for ACE2 binding, RBD expression, and antibody escape in the SARS-CoV-2 Omicron BA.1 and BA.2 receptor-binding domains by Starr, Tyler N, Greaney, Allison J, Stewart, Cameron M, Walls, Alexandra C, Hannon, William W, Veesler, David, Bloom, Jesse D

“…SARS-CoV-2 continues to acquire mutations in the spike receptor-binding domain (RBD) that impact ACE2 receptor binding, folding stability, and antibody…”
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Reconciling disparate estimates of viral genetic diversity during human influenza infections by Xue, Katherine S., Bloom, Jesse D.

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Mapping mutations to the SARS-CoV-2 RBD that escape binding by different classes of antibodies by Greaney, Allison J., Starr, Tyler N., Barnes, Christopher O., Weisblum, Yiska, Schmidt, Fabian, Caskey, Marina, Gaebler, Christian, Cho, Alice, Agudelo, Marianna, Finkin, Shlomo, Wang, Zijun, Poston, Daniel, Muecksch, Frauke, Hatziioannou, Theodora, Bieniasz, Paul D., Robbiani, Davide F., Nussenzweig, Michel C., Bjorkman, Pamela J., Bloom, Jesse D.

“…Monoclonal antibodies targeting a variety of epitopes have been isolated from individuals previously infected with SARS-CoV-2, but the relative contributions…”
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Stability-mediated epistasis constrains the evolution of an influenza protein by Gong, Lizhi Ian, Suchard, Marc A, Bloom, Jesse D

“…John Maynard Smith compared protein evolution to the game where one word is converted into another a single letter at a time, with the constraint that all…”
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Deep mutational scanning of hemagglutinin helps predict evolutionary fates of human H3N2 influenza variants by Lee, Juhye M., Huddleston, John, Doud, Michael B., Hooper, Kathryn A., Wu, Nicholas C., Bedford, Trevor, Bloom, Jesse D.

“…Human influenza virus rapidly accumulates mutations in its major surface protein hemagglutinin (HA). The evolutionary success of influenza virus lineages…”
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Accurate Measurement of the Effects of All Amino-Acid Mutations on Influenza Hemagglutinin by Doud, Michael B, Bloom, Jesse D

“…Influenza genes evolve mostly via point mutations, and so knowing the effect of every amino-acid mutation provides information about evolutionary paths…”
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Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays by Crawford, Katharine H D, Eguia, Rachel, Dingens, Adam S, Loes, Andrea N, Malone, Keara D, Wolf, Caitlin R, Chu, Helen Y, Tortorici, M Alejandra, Veesler, David, Murphy, Michael, Pettie, Deleah, King, Neil P, Balazs, Alejandro B, Bloom, Jesse D

“…SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera…”
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Experimental Estimation of the Effects of All Amino-Acid Mutations to HIV's Envelope Protein on Viral Replication in Cell Culture by Haddox, Hugh K, Dingens, Adam S, Bloom, Jesse D

“…HIV is notorious for its capacity to evade immunity and anti-viral drugs through rapid sequence evolution. Knowledge of the functional effects of mutations to…”
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An Antigenic Atlas of HIV-1 Escape from Broadly Neutralizing Antibodies Distinguishes Functional and Structural Epitopes by Dingens, Adam S., Arenz, Dana, Weight, Haidyn, Overbaugh, Julie, Bloom, Jesse D.

“…Anti-HIV broadly neutralizing antibodies (bnAbs) have revealed vaccine targets on the virus’s envelope (Env) protein and are themselves promising…”
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Within-Host Evolution of Human Influenza Virus by Xue, Katherine S., Moncla, Louise H., Bedford, Trevor, Bloom, Jesse D.

“…The rapid global evolution of influenza virus begins with mutations that arise de novo in individual infections, but little is known about how evolution occurs…”
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