Clomiphene citrate ameliorated lead acetate-induced reproductive toxicity in male Wistar rats

The current study investigated the effects of clomiphene citrate on the hypothalamic-pituitary-testicular axis, steroidogenesis, sperm parameters, and testicular antioxidant enzyme activity of male Wistar rats submitted to lead acetate (Pb)-induced reproductive toxicity. Twenty adult male Wistar rat...

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Bibliographic Details
Published in:	JBRA assisted reproduction Vol. 23; no. 4; pp. 336 - 343
Main Authors:	Wahab, Oyeyemi A, Princely, Anyanwu C, Oluwadamilare, Akinola A, Ore-Oluwapo, Daramola O, Blessing, Alli O, Alfred, Ehiaghe F
Format:	Journal Article
Language:	English
Published:	Brazil Sociedade Brasileira de Reprodução Humana (Brazilian Society of Assisted Reproduction) 2019 Brazilian Society of Assisted Reproduction
Subjects:	Androgens Animals Antioxidants Cosmetics Dehydrogenases Enzymes Food contamination & poisoning Free radicals Histology Infertility Lipid peroxidation Lipids Motility Original Oxidative stress Physiology Rodents Sperm Stains & staining Testosterone Nigeria clomiphene citrate gonadotropins 17β-hydroxysteroid dehydrogenase lead acetate sperm count
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Summary:	The current study investigated the effects of clomiphene citrate on the hypothalamic-pituitary-testicular axis, steroidogenesis, sperm parameters, and testicular antioxidant enzyme activity of male Wistar rats submitted to lead acetate (Pb)-induced reproductive toxicity. Twenty adult male Wistar rats were divided into four groups of equal size as follows: Control; Clomid (0.35 mg/kg); Pb (10 mg/kg); and Clomid + Pb. Serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone, testicular 17-β hydroxysteroid dehydrogenase (17-β HSD) activity, androgen receptors, catalase activity, superoxide dismutase (SOD), malondialdehyde (MDA), sperm motility, viability, counts and morphology were estimated after oral administration of Clomid and/or lead acetate for 35 consecutive days. Data were analyzed using ANOVA at p<0.05. Lead acetate significantly decreased (p<0.05) serum LH and testosterone levels, testicular 17β-HSD activity, androgen receptor expression, sperm motility, viability, counts, catalase activity, and SOD when compared with controls. Abnormal sperm morphology and MDA were significantly increased (p<0.05) in the Pb group compared with controls. Clomid co-administrated with lead acetate significantly increased (p<0.05) serum LH, testosterone levels, testicular 17β-HSD, androgen receptor expression, sperm motility and viability when compared with the group given lead acetate. The present study suggests that clomiphene citrate may stimulate testicular testosterone synthesis, sperm motility and viability via luteinizing hormone in a context of lead acetate-induced reproductive toxicity.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1518-0557 1517-5693 1518-0557
DOI:	10.5935/1518-0557.20190038