Search Results - "Blankenship, Dale T."

Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris by Sreekrishna, Koti, Brankamp, Robert G., Kropp, Keith E., Blankenship, Dale T., Tsay, Jiu-Tsair, Smith, Phil L., Wierschke, Jonathan D., Subramaniam, Arun, Birkenberger, Lori A.

“…Numerous heterologous proteins have been produced at greater than gram per liter levels in the methylotrophic yeast, Pichia pastoris, using the methanol…”
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Isolation and characterization of four heparin-binding cyanogen bromide peptides of human plasma apolipoprotein B by Hirose, Nobuyoshi, Blankenship, Dale T, Krivanek, Jackson, Richard L, Cardin, Alan D

“…Apolipoprotein B-100 (apoB-100) is the major protein constituent of human plasma low-density lipoproteins (LDL). On the basis of its amino acid sequence [Chen,…”
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Binding of a high reactive heparin to human apolipoprotein E: identification of two heparin-binding domains by Cardin, A D, Hirose, N, Blankenship, D T, Jackson, R L, Harmony, J A, Sparrow, D A, Sparrow, J T

“…Ligand-blotting and dot-blotting procedures were used to investigate the binding of [125I]-heparin to apolipoprotein E, its thrombin fragments E22 (residues…”
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Purification and characterization of dihydrofolate reductase from methotrexate-resistant human lymphoblastoid cells by Delcamp, Tavner J, Susten, Sandra S, Blankenship, Dale T, Freisheim, James H

“…Dihydrofolate reductase has been isolated from methotrexate-resistant WIL2 human lymphoblastoid cells. This subline produces ca. 150 times more enzyme than the…”
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Primary structure of chicken liver dihydrofolate reductase by Kumar, A. Ashok, Blankenship, Dale T, Kaufman, Bernard T, Freisheim, James H

“…The complete covalent structure of dihydrofolate reductase from chicken liver is described. The S-carboxymethylated protein was subjected to cleavage by…”
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Expression of a Synthetic Gene Encoding the Anticoagulant-Antimetastatic Protein Ghilanten by the Methylotropic YeastPichia pastoris by Brankamp, Robert G., Sreekrishna, Koti, Smith, Philip L., Blankenship, Dale T., Cardin, Alan D.

“…Ghilantens are a family of cysteine-rich inhibitors of the clotting enzyme, factor Xa, that are produced in the salivary glands of the South American…”
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Crystallographic structure of human gamma-thrombin by T J Rydel, M Yin, K P Padmanabhan, D T Blankenship, A D Cardin, P E Correa, J W Fenton, 2nd, A Tulinsky

“…In an effort to prepare crystals and determine the structure of alpha-thrombin complexed to a synthetic peptide inhibitor (MDL-28050) of the hirudin 54-65…”
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High-sensitivity amino acid analysis by derivatization with O-phthalaldehyde and 9-fluorenylmethyl chloroformate using fluorescence detection: applications in protein structure determination by Blankenship, D T, Krivanek, M A, Ackermann, B L, Cardin, A D

“…An amino acid analysis method using a commercially available analyzer that accurately quantitates protein-derived amino acids in the 10-100 pmol range is…”
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Curtatoxins: neurotoxic insecticidal polypeptides isolated from the funnel-web spider Hololena curta by Stapleton, A. (Merrell Dow Research Institute, Cincinnati, OH), Blankenship, D.T, Ackermann, B.L, Chen, T.M, Gorder, G.W, Manley, G.D, Palfreyman, M.G, Coutant, J.E, Cardin, A.D

“…Three polypeptide neurotoxins (curtatoxins) were isolated from the venom of the spider Hololena curta by reverse-phase high performance liquid chromatography,…”
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Amino acid sequence of ghilanten: anticoagulant-antimetastatic principle of the South American leech, Haementeria ghilianii by Blankenship, D T, Brankamp, R G, Manley, G D, Cardin, A D

“…This study reports the amino acid sequence of ghilanten, an anticoagulant-antimetastatic principle of the hematophagous leech, Haementeria ghilianii. Ghilanten…”
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Stilbene disulfonic acids. CD4 antagonists that block human immunodeficiency virus type-1 growth at multiple stages of the virus life cycle by CARDIN, A. D, SMITH, P. L, HYDE, L, BLANKENSHIP, D. T, BOWLIN, T. L, SCHROEDER, K, STAUDERMAN, K. A, TAYLOR, D. L, TYMS, A. S

“…The stilbene disulfonic acids 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), 4,4'-diisothiocyanatodihydrostilbene-2,2'-disulfonic acid and,…”
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In vitro processing of rANF 7–28-NH 2 by rat kidney homogenates by Berman, Judd M., Pelton, John T., Cardin, Alan D., Blankenship, Dale T., Hassman, C.Fred, M. Chen, Teng Man

“…The major rat kidney in vitro degradation products of the rat atrial natriuretic factor analog, H-Cys…”
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Rapid purification and revised amino-terminal sequence of hirudin: a specific thrombin inhibitor of the bloodsucking leech by Mao, S J, Yates, M T, Blankenship, D T, Cardin, A D, Krstenansky, J L, Lovenberg, W, Jackson, R L

“…Hirudin is a specific polypeptide thrombin inhibitor consisting of 65 amino acids that is produced by the leech, Hirudo medicinalis. We describe a rapid method…”
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In vitro processing of rANF7–28‐NH2 by rat kidney homogenates by Berman, Judd M., Pelton, John T., Cardin, Alan D., Blankenship, Dale T., Hassman, C.Fred, M. Chen, Teng Man

“…The major rat kidney in vitro degradation products of the rat atrial natriuretic factor analog,…”
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Lysine and ornithine analogs of methotrexate as inhibitors of dihydrofolate reductase by Kempton, Robert J., Black, Angelique M., Anstead, Gregory M., Kumar, A. Ashok, Blankenship, Dale T., Freisheim, James H.

“…The ornithine (6a) and lysine (6b) analogues of methotrexate (1) have been synthesized via condensation of 4-amino-4-deoxy-N10-methylpteroic acid (2) with N…”
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Mechanism of enhanced kinin release from high molecular weight kininogen by plasma kallikrein after its exposure to plasmin by Kleniewski, J, Blankenship, D T, Cardin, A D, Donaldson, V

“…When purified high molecular weight kininogen was incubated with streptokinase-activated plasmin and kallikrein, a larger amount of kinin was released than…”
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Reduced immunoregulatory potency of low density lipoproteins with selectively modified arginine and lysine residues of apolipoprotein B by Noel, J G, Hui, D Y, Blankenship, D T, Harmony, J A

“…Human plasma low density lipoproteins (LDL) suppress lymphocyte activation in vitro by inhibiting the early, membrane-associated events such as…”
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