Search Results - "Blanchard, Scott"

CFTR function, pathology and pharmacology at single-molecule resolution by Levring, Jesper, Terry, Daniel S., Kilic, Zeliha, Fitzgerald, Gabriel, Blanchard, Scott C., Chen, Jue

“…The cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel that regulates salt and fluid homeostasis across epithelial membranes 1 …”
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Aminoglycoside interactions and impacts on the eukaryotic ribosome by Prokhorova, Irina, Altman, Roger B., Djumagulov, Muminjon, Shrestha, Jaya P., Urzhumtsev, Alexandre, Ferguson, Angelica, Chang, Cheng-Wei Tom, Yusupov, Marat, Blanchard, Scott C., Yusupova, Gulnara

“…Aminoglycosides are chemically diverse, broad-spectrum antibiotics that target functional centers within the bacterial ribosome to impact all four principle…”
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Transport dynamics in a glutamate transporter homologue by Akyuz, Nurunisa, Altman, Roger B., Blanchard, Scott C., Boudker, Olga

“…Single-molecule fluorescence resonance energy transfer imaging of a bacterial glutamate transporter reveals how the transport domains move. Glutamate transport…”
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A Much-Needed Boost for the Dwindling Antibiotic Pipeline by Blanchard, Scott C.

“…In this issue of Molecular Cell, Pantel et al. (2018) identify and synthetically optimize a novel ribosome-targeting antimicrobial from a potentially rich new…”
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Single-molecule analysis of ligand efficacy in β2AR–G-protein activation by Gregorio, G. Glenn, Masureel, Matthieu, Hilger, Daniel, Terry, Daniel S., Juette, Manuel, Zhao, Hong, Zhou, Zhou, Perez-Aguilar, Jose Manuel, Hauge, Maria, Mathiasen, Signe, Javitch, Jonathan A., Weinstein, Harel, Kobilka, Brian K., Blanchard, Scott C.

“…G-protein-coupled receptor (GPCR)-mediated signal transduction is central to human physiology and disease intervention, yet the molecular mechanisms…”
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The Contribution of Reactive Oxygen Species to the Photobleaching of Organic Fluorophores by Zheng, Qinsi, Jockusch, Steffen, Zhou, Zhou, Blanchard, Scott C.

“…Photoexcitation of fluorophores commonly used for biological imaging applications generates reactive oxygen species (ROS) which can cause bleaching of the…”
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Structural basis for potent inhibitory activity of the antibiotic tigecycline during protein synthesis by Jenner, Lasse, Starosta, Agata L., Terry, Daniel S., Mikolajka, Aleksandra, Filonava, Liudmila, Yusupov, Marat, Blanchard, Scott C., Wilson, Daniel N., Yusupova, Gulnara

“…Here we present an X-ray crystallography structure of the clinically relevant tigecycline antibiotic bound to the 70S ribosome. Our structural and biochemical…”
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Conformational dynamics of single HIV-1 envelope trimers on the surface of native virions by Munro, James B., Gorman, Jason, Ma, Xiaochu, Zhou, Zhou, Arthos, James, Burton, Dennis R., Koff, Wayne C., Courter, Joel R., Smith, Amos B., Kwong, Peter D., Blanchard, Scott C., Mothes, Walther

“…The HIV-1 envelope (Env) mediates viral entry into host cells. To enable the direct imaging of conformational dynamics within Env, we introduced fluorophores…”
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Real-Time Conformational Dynamics of SARS-CoV-2 Spikes on Virus Particles by Lu, Maolin, Uchil, Pradeep D., Li, Wenwei, Zheng, Desheng, Terry, Daniel S., Gorman, Jason, Shi, Wei, Zhang, Baoshan, Zhou, Tongqing, Ding, Shilei, Gasser, Romain, Prévost, Jérémie, Beaudoin-Bussières, Guillaume, Anand, Sai Priya, Laumaea, Annemarie, Grover, Jonathan R., Liu, Lihong, Ho, David D., Mascola, John R., Finzi, Andrés, Kwong, Peter D., Blanchard, Scott C., Mothes, Walther

“…The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) mediates viral entry into cells and is critical for vaccine development against…”
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High-resolution structure of the Escherichia coli ribosome by Noeske, Jonas, Wasserman, Michael R, Terry, Daniel S, Altman, Roger B, Blanchard, Scott C, Cate, Jamie H D

“…High-resolution structure of the E. coli ribosome highlights rRNA and protein modifications and provides details on solvation characteristics and the…”
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Multiperspective smFRET reveals rate-determining late intermediates of ribosomal translocation by Wasserman, Michael R, Alejo, Jose L, Altman, Roger B, Blanchard, Scott C

“…Monitoring ribosomal translocation from five structural perspectives with pre–steady state smFRET defines intramolecular conformational events within the…”
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Ultra-stable organic fluorophores for single-molecule research by Zheng, Qinsi, Juette, Manuel F, Jockusch, Steffen, Wasserman, Michael R, Zhou, Zhou, Altman, Roger B, Blanchard, Scott C

“…Fluorescence provides a mechanism for achieving contrast in biological imaging that enables investigations of molecular structure, dynamics, and function at…”
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Structural basis of early translocation events on the ribosome by Rundlet, Emily J., Holm, Mikael, Schacherl, Magdalena, Natchiar, S. Kundhavai, Altman, Roger B., Spahn, Christian M. T., Myasnikov, Alexander G., Blanchard, Scott C.

“…Peptide-chain elongation during protein synthesis entails sequential aminoacyl-tRNA selection and translocation reactions that proceed rapidly (2–20 per…”
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Structural basis for CFTR inhibition by CFTRinh-172 by Young, Paul G, Levring, Jesper, Fiedorczuk, Karol, Blanchard, Scott C, Chen, Jue

“…The cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel that regulates electrolyte and fluid balance in epithelial tissues. While…”
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Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale by Juette, Manuel F, Terry, Daniel S, Wasserman, Michael R, Altman, Roger B, Zhou, Zhou, Zhao, Hong, Blanchard, Scott C

“…This report describes an imaging and analysis platform enabling high-throughput single-molecule fluorescence investigations of fast, transient molecular…”
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Enabling multiperspective single-molecule FRET by Kiselev, Roman, Modak, Arnab, Terry, Daniel, Borgia, Alessandro, Altman, Roger, Blanchard, Scott C.

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Miscoding-induced stalling of substrate translocation on the bacterial ribosome by Alejo, Jose L., Blanchard, Scott C.

“…Directional transit of the ribosome along the messenger RNA (mRNA) template is a key determinant of the rate and processivity of protein synthesis. Imaging of…”
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Structural basis for CFTR inhibition by CFTR inh -172 by Young, Paul G, Levring, Jesper, Fiedorczuk, Karol, Blanchard, Scott C, Chen, Jue

“…The cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel that regulates electrolyte and fluid balance in epithelial tissues. While…”
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Assembly and diploid architecture of an individual human genome via single-molecule technologies by Pendleton, Matthew, Sebra, Robert, Pang, Andy Wing Chun, Ummat, Ajay, Franzen, Oscar, Rausch, Tobias, Stütz, Adrian M, Stedman, William, Anantharaman, Thomas, Hastie, Alex, Dai, Heng, Fritz, Markus Hsi-Yang, Cao, Han, Cohain, Ariella, Deikus, Gintaras, Durrett, Russell E, Blanchard, Scott C, Altman, Roger, Chin, Chen-Shan, Guo, Yan, Paxinos, Ellen E, Korbel, Jan O, Darnell, Robert B, McCombie, W Richard, Kwok, Pui-Yan, Mason, Christopher E, Schadt, Eric E, Bashir, Ali

“…A combination of single-molecule long-read sequencing, single-molecule genome mapping and short-read sequencing provides reference-quality de novo assemblies…”
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Conformational capture of the SAM-II riboswitch by Haller, Andrea, Rieder, Ulrike, Aigner, Michaela, Blanchard, Scott C, Micura, Ronald

“…SAM riboswitches are RNA elements that regulate bacterial gene expression in response to binding of the small-molecule metabolite, S -adenosylmethionine. The…”
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