Search Results - "Blair, Rebecca H."

The HMGB1 C-Terminal Tail Regulates DNA Bending by Blair, Rebecca H., Horn, Abigail E., Pazhani, Yogitha, Grado, Lizbeth, Goodrich, James A., Kugel, Jennifer F.

“…High mobility group box protein 1 (HMGB1) is an architectural protein that facilitates the formation of protein–DNA assemblies involved in transcription,…”
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Analytical Performance of a Multiplexed Microarray Assay for Rapid Identification and Quantification of a Multivalent mRNA Vaccine by Gerold, Megan N, Toth, Evan, Blair, Rebecca H, Gao, Rachel Y, Nadkarni, Durgesh V, Barua, Sutapa, Woods, Joshua, Rowlen, Kathy L, Dawson, Erica D

“…mRNA vaccines were highly effective in response to the COVID-19 pandemic, making them an attractive platform to address cancers and other infectious diseases…”
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Rapid Identity and Quantity CQA Test for Multivalent mRNA Drug Product Formulations by Gao, Rachel Y, Riley, Christine M, Toth, Evan, Blair, Rebecca H, Gerold, Megan N, McCormick, Caitlin, Taylor, Amber W, Hu, Tianjing, Rowlen, Kathy L, Dawson, Erica D

“…The COVID-19 pandemic highlighted mRNA as a promising platform for vaccines and therapeutics. Many of the analytical tools used to characterize the critical…”
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Multiplexed, microscale, microarray-based serological assay for antibodies against all human-relevant coronaviruses by Dawson, Erica D., Kuck, Laura R., Blair, Rebecca H., Taylor, Amber W., Toth, Evan, Knight, Vijaya, Rowlen, Kathy L.

“…•Quantitative assay with LOD from 0.32 to 2.0 ng/mL (determined with mAbs).•Linear dynamic ranges were 76- to 911-fold, depending on antigen.•Accuracy (%…”
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Single-Molecule Fluorescence Resonance Energy Transfer Shows Uniformity in TATA Binding Protein-Induced DNA Bending and Heterogeneity in Bending Kinetics by Blair, Rebecca H, Goodrich, James A, Kugel, Jennifer F

“…TATA binding protein (TBP) is a key component of the eukaryotic RNA polymerase II transcription machinery that binds to TATA boxes located in the core promoter…”
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Analytical evaluation of the microarray-based FluChip-8G Influenza A+B Assay by Taylor, Amber W., Dawson, Erica D., Blair, Rebecca H., Johnson, James E., Slinskey, Amelia H., Smolak, Andrew W., Toth, Evan, Liikanen, Kyle, Stoughton, Robert S., Smith, Catherine, Talbot, Sarah, Rowlen, Kathy L.

“…•Multiplex RT-PCR microarray assay for influenza typing and subtyping was evaluated.•Limits of detection were ∼200 genome copies/reaction, depending on…”
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Clinical validation of the FluChip-8G Influenza A+B Assay for influenza type and subtype identification by Blair, Rebecca H., Dawson, Erica D., Taylor, Amber W., Johnson, James E., Slinskey, Amelia H., O’Neil, Kelly, Smolak, Andrew W., Toth, Evan, Liikanen, Kyle, Stoughton, Robert S., Smith, Catherine B., Talbot, Sarah, Rowlen, Kathy L.

“…•The clinical performance of the FluChip-8G Influenza A+B Assay was evaluated.•Sensitivities of seasonal influenza viruses are greater than 91%.•Testing…”
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FluChip‐8G Insight: HA and NA subtyping of potentially pandemic influenza A viruses in a single assay by Toth, Evan, Dawson, Erica D., Taylor, Amber W., Stoughton, Robert S., Blair, Rebecca H., Johnson, James E., Slinskey, Amelia, Fessler, Ryan, Smith, Catherine B., Talbot, Sarah, Rowlen, Kathy

“…Background Global influenza surveillance in humans and animals is a critical component of pandemic preparedness. The FluChip‐8G Insight assay was developed to…”
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Using FRET to monitor protein-induced DNA bending: the TBP-TATA complex as a model system by Blair, Rebecca H, Goodrich, James A, Kugel, Jennifer F

“…Proteins that bind to DNA can elicit changes in DNA conformation, such as bending and looping, which are important signals for later events such as…”
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Real-time quantification of RNA polymerase activity using a “broken beacon” by Blair, Rebecca H., Rosenblum, Eric S., Dawson, Erica D., Kuchta, Robert D., Kuck, Laura R., Rowlen, Kathy L.

“…A novel assay using a hybridization-based method was developed for real-time monitoring of RNA synthesis. In this work, a “broken beacon” in which the fluor…”
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Single molecule FRET shows uniformity in TBP-induced DNA bending and heterogeneity in bending kinetics by Blair, Rebecca H., Goodrich, James A., Kugel, Jennifer F.

“…TATA binding protein (TBP) is a key component of the eukaryotic RNA polymerase II (Pol II) transcription machinery that binds to TATA boxes located in the core…”
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