11C-radiolabeled aptamer for imaging of tumors and metastases using positron emission tomography- computed tomography

Identification of primary tumors and metastasis sites is an essential step in cancer diagnostics and the following treatment. Positron emission tomography-computed tomography (PET/CT) is one of the most reliable methods for scanning the whole organism for malignancies. In this work, we synthesized a...

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Published in:	Molecular therapy. Nucleic acids Vol. 26; pp. 1159 - 1172
Main Authors:	Ozerskaya, Anastasia V., Zamay, Tatiana N., Kolovskaya, Olga S., Tokarev, Nikolay A., Belugin, Kirill V., Chanchikova, Natalia G., Badmaev, Oleg N., Zamay, Galina S., Shchugoreva, Irina A., Moryachkov, Roman V., Zabluda, Vladimir N., Khorzhevskii, Vladimir A., Shepelevich, Nikolay, Gappoev, Stanislav V., Karlova, Elena A., Saveleva, Anastasia S., Volzhentsev, Alexander A., Blagodatova, Anna N., Lukyanenko, Kirill A., Veprintsev, Dmitry V., Smolyarova, Tatyana E., Tomilin, Felix N., Zamay, Sergey S., Silnikov, Vladimir N., Berezovski, Maxim V., Kichkailo, Anna S.
Format:	Journal Article
Language:	English
Published:	Elsevier Inc 03-12-2021 American Society of Gene & Cell Therapy Elsevier
Subjects:	11C radiolabeling DNA aptamers Ehrlich ascites carcinoma in vivo imaging metastasis Original PET/CT radiopharmaceuticals radiopharmaceuticals DNA aptamers metastasis PET/CT Ehrlich ascites carcinoma 11C radiolabeling in vivo imaging
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Summary:	Identification of primary tumors and metastasis sites is an essential step in cancer diagnostics and the following treatment. Positron emission tomography-computed tomography (PET/CT) is one of the most reliable methods for scanning the whole organism for malignancies. In this work, we synthesized an 11C-labeled oligonucleotide primer and hybridized it to an anti-cancer DNA aptamer. The 11C-aptamer was applied for in vivo imaging of Ehrlich ascites carcinoma and its metastases in mice using PET/CT. The imaging experiments with the 11C-aptamer determined very small primary and secondary tumors of 3 mm2 and less. We also compared 11C imaging with the standard radiotracer, 2-deoxy-2-[fluorine-18]fluoro-D-glucose (18F-FDG), and found better selectivity of the 11C-aptamer to metastatic lesions in the metabolically active organs than 18F-FDG. 11C radionuclide with an ultra-short (20.38 min) half-life is considered safest for PET/CT imaging and does not cause false-positive results in heart imaging. Its combination with aptamers gives us high-specificity and high-contrast imaging of cancer cells and can be applied for PET/CT-guided drug delivery in cancer therapies. [Display omitted] In this work, a DNA aptamer was labeled with 11C radionuclide and applied for cancer imaging in mice using positron emission tomography-computed tomography (PET/CT). The imaging experiments with the 11C-aptamer determined very small primary tumors and metastases of 3 mm2 and less.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2162-2531 2162-2531
DOI:	10.1016/j.omtn.2021.10.020