Extraordinary salmon growth

For the production of transgenic Pacific salmon, we have circumvented some problems by developing a gene construct (pOnMTGH1) where all genetic elements were derived from sockeye salmon. This "all-salmon" construct consists of the metallothionein-B promoter fused to the full-length type-1...

Full description

Saved in:

Bibliographic Details
Published in:	Nature (London) Vol. 371; no. 6494; pp. 209 - 210
Main Authors:	Yesaki, T.Y, Blagi, C.A, Devlin, R.H, Donaldson, E.M, Swanson, P, Chan, W.K
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group 15-09-1994
Subjects:	body weight Freshwater gene construct growth rate Oncorhynchus kisutch Salmon transgenic animals
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	For the production of transgenic Pacific salmon, we have circumvented some problems by developing a gene construct (pOnMTGH1) where all genetic elements were derived from sockeye salmon. This "all-salmon" construct consists of the metallothionein-B promoter fused to the full-length type-1 growth hormone gene, and is identical in concept to the construct first used to stimulate growth in transgenic mice. We microinjected linear pOnMTGH1 DNA into the blastodisc region (animal pole) of coho salmon eggs that were developmentally arrested immediately after fertilization. On average, the transgenic salmon were more than 11-fold heavier than non-transgenic controls, with a range from no growth stimulation to one individual 37 times larger than controls.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0028-0836 1476-4687
DOI:	10.1038/371209a0