Search Results - "Blacker, Thomas S."

Cellular and molecular mechanisms of mitochondrial function by Osellame, Laura D., B.Sc (Hons), Ph.D, Blacker, Thomas S., MPhys, MRes, Duchen, Michael R., BA (Oxon), MBBS, MRCP, PhD, MAE, FSB

“…Mitochondria are membrane bound organelles present in almost all eukaryotic cells. Responsible for orchestrating cellular energy production, they are central…”
Get full text

Reversal of Mitochondrial Transhydrogenase Causes Oxidative Stress in Heart Failure by Nickel, Alexander G., von Hardenberg, Albrecht, Hohl, Mathias, Löffler, Joachim R., Kohlhaas, Michael, Becker, Janne, Reil, Jan-Christian, Kazakov, Andrey, Bonnekoh, Julia, Stadelmaier, Moritz, Puhl, Sarah-Lena, Wagner, Michael, Bogeski, Ivan, Cortassa, Sonia, Kappl, Reinhard, Pasieka, Bastian, Lafontaine, Michael, Lancaster, C. Roy D., Blacker, Thomas S., Hall, Andrew R., Duchen, Michael R., Kästner, Lars, Lipp, Peter, Zeller, Tanja, Müller, Christian, Knopp, Andreas, Laufs, Ulrich, Böhm, Michael, Hoth, Markus, Maack, Christoph

“…Mitochondrial reactive oxygen species (ROS) play a central role in most aging-related diseases. ROS are produced at the respiratory chain that demands NADH for…”
Get full text

Separating NADH and NADPH fluorescence in live cells and tissues using FLIM by Blacker, Thomas S., Mann, Zoe F., Gale, Jonathan E., Ziegler, Mathias, Bain, Angus J., Szabadkai, Gyorgy, Duchen, Michael R.

“…NAD is a key determinant of cellular energy metabolism. In contrast, its phosphorylated form, NADP, plays a central role in biosynthetic pathways and…”
Get full text

Diabetes causes marked inhibition of mitochondrial metabolism in pancreatic β-cells by Haythorne, Elizabeth, Rohm, Maria, van de Bunt, Martijn, Brereton, Melissa F., Tarasov, Andrei I., Blacker, Thomas S., Sachse, Gregor, Silva dos Santos, Mariana, Terron Exposito, Raul, Davis, Simon, Baba, Otto, Fischer, Roman, Duchen, Michael R., Rorsman, Patrik, MacRae, James I., Ashcroft, Frances M.

“…Diabetes is a global health problem caused primarily by the inability of pancreatic β-cells to secrete adequate levels of insulin. The molecular mechanisms…”
Get full text

The mitochondrial calcium uniporter regulates breast cancer progression via HIF‐1α by Tosatto, Anna, Sommaggio, Roberta, Kummerow, Carsten, Bentham, Robert B, Blacker, Thomas S, Berecz, Tunde, Duchen, Michael R, Rosato, Antonio, Bogeski, Ivan, Szabadkai, Gyorgy, Rizzuto, Rosario, Mammucari, Cristina

“…Triple‐negative breast cancer (TNBC) represents the most aggressive breast tumor subtype. However, the molecular determinants responsible for the metastatic…”
Get full text

Nrf2 regulates glucose uptake and metabolism in neurons and astrocytes by Esteras, Noemí, Blacker, Thomas S., Zherebtsov, Evgeny A., Stelmashuk, Olga A., Zhang, Ying, Wigley, W. Christian, Duchen, Michael R., Dinkova-Kostova, Albena T., Abramov, Andrey Y.

“…The transcription factor Nrf2 and its repressor Keap1 mediate cell stress adaptation by inducing expression of genes regulating cellular detoxification,…”
Get full text

Gradients of glucose metabolism regulate morphogen signalling required for specifying tonotopic organization in the chicken cochlea by O'Sullivan, James D B, Blacker, Thomas S, Scott, Claire, Chang, Weise, Ahmed, Mohi, Yianni, Val, Mann, Zoe F

“…In vertebrates with elongated auditory organs, mechanosensory hair cells (HCs) are organised such that complex sounds are broken down into their component…”
Get full text

Photoactivated cell-killing involving a low molecular weight, donor-acceptor diphenylacetylene by Chisholm, David R, Lamb, Rebecca, Pallett, Tommy, Affleck, Valerie, Holden, Claire, Marrison, Joanne, O'Toole, Peter, Ashton, Peter D, Newling, Katherine, Steffen, Andreas, Nelson, Amanda K, Mahler, Christoph, Valentine, Roy, Blacker, Thomas S, Bain, Angus J, Girkin, John, Marder, Todd B, Whiting, Andrew, Ambler, Carrie A

“…Photoactivation of photosensitisers can be utilised to elicit the production of ROS, for potential therapeutic applications, including the destruction of…”
Get full text

CHCHD4 regulates tumour proliferation and EMT-related phenotypes, through respiratory chain-mediated metabolism by Thomas, Luke W, Esposito, Cinzia, Stephen, Jenna M, Costa, Ana S H, Frezza, Christian, Blacker, Thomas S, Szabadkai, Gyorgy, Ashcroft, Margaret

“…Mitochondrial oxidative phosphorylation (OXPHOS) via the respiratory chain is required for the maintenance of tumour cell proliferation and regulation of…”
Get full text

NAD(P)H binding configurations revealed by time-resolved fluorescence and two-photon absorption by Blacker, Thomas S., Duchen, Michael R., Bain, Angus J.

“…NADH and NADPH play key roles in the regulation of metabolism. Their endogenous fluorescence is sensitive to enzyme binding, allowing changes in cellular…”
Get full text

Polarized Two-Photon Absorption and Heterogeneous Fluorescence Dynamics in NAD(P)H by Blacker, Thomas S, Nicolaou, Nick, Duchen, Michael R, Bain, Angus J

“…Two-photon absorption (2PA) finds widespread application in biological systems, which frequently exhibit heterogeneous fluorescence decay dynamics…”
Get full text

Investigating mitochondrial redox state using NADH and NADPH autofluorescence by Blacker, Thomas S., Duchen, Michael R.

“…The redox states of the NAD and NADP pyridine nucleotide pools play critical roles in defining the activity of energy producing pathways, in driving oxidative…”
Get full text

Multiphoton NAD(P)H FLIM reveals metabolic changes in individual cell types of the intact cochlea upon sensorineural hearing loss by Majumder, Paromita, Blacker, Thomas S., Nolan, Lisa S., Duchen, Michael R., Gale, Jonathan E.

“…An increasing volume of data suggests that changes in cellular metabolism have a major impact on the health of tissues and organs, including in the auditory…”
Get full text

Decellularized Cartilage Directs Chondrogenic Differentiation: Creation of a Fracture Callus Mimetic by Vas, Wollis J, Shah, Mittal, Blacker, Thomas S, Duchen, Michael R, Sibbons, Paul, Roberts, Scott J

“…Complications that arise from impaired fracture healing have considerable socioeconomic implications. Current research in the field of bone tissue engineering…”
Get more information

Cellular localisation of structurally diverse diphenylacetylene fluorophores by Chisholm, David R, Hughes, Joshua G, Blacker, Thomas S, Humann, Rachel, Adams, Candace, Callaghan, Daniel, Pujol, Alba, Lembicz, Nicola K, Bain, Angus J, Girkin, John M, Ambler, Carrie A, Whiting, Andrew

“…Fluorescent probes are increasingly used as reporter molecules in a wide variety of biophysical experiments, but when designing new compounds it can often be…”
Get more information

Investigating State Restriction in Fluorescent Protein FRET Using Time-Resolved Fluorescence and Anisotropy by Blacker, Thomas S., Chen, WeiYue, Avezov, Edward, Marsh, Richard J., Duchen, Michael R., Kaminski, Clemens F., Bain, Angus J.

“…Most fluorescent proteins exhibit multiexponential fluorescence decays, indicating a heterogeneous excited state population. FRET between fluorescent proteins…”
Get full text

Metabolic Profiling of Live Cancer Tissues Using NAD(P)H Fluorescence Lifetime Imaging by Blacker, Thomas S, Sewell, Michael D E, Szabadkai, Gyorgy, Duchen, Michael R

“…Altered metabolism is a hallmark of cancer, both resulting from and driving oncogenesis. The NAD and NADP redox couples play a key role in a large number of…”
Get more information

Assessment of Cellular Redox State Using NAD(P)H Fluorescence Intensity and Lifetime by Blacker, Thomas S, Berecz, Tunde, Duchen, Michael R, Szabadkai, Gyorgy

“…NADH and NADPH are redox cofactors, primarily involved in catabolic and anabolic metabolic processes respectively. In addition, NADPH plays an important role…”
Get full text

Activated barrier crossing dynamics in the non-radiative decay of NADH and NADPH by Blacker, Thomas S., Marsh, Richard J., Duchen, Michael R., Bain, Angus J.

“…[Display omitted] ► NADH and NADPH have a high rate of non-radiative excited state decay. ► Conformational relaxation is shown to be a significant…”
Get full text

Photoactivated cell-killing involving a low molecular weight, donor–acceptor diphenylacetylene† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c9sc00199a by Chisholm, David R., Lamb, Rebecca, Pallett, Tommy, Affleck, Valerie, Holden, Claire, Marrison, Joanne, O'Toole, Peter, Ashton, Peter D., Newling, Katherine, Steffen, Andreas, Nelson, Amanda K., Mahler, Christoph, Valentine, Roy, Blacker, Thomas S., Bain, Angus J., Girkin, John, Marder, Todd B., Whiting, Andrew, Ambler, Carrie A.

“…Drug-like, donor–acceptor diphenylacetylenes cause efficient cell death upon photoactivation and hence have potential phototherapeutic applications…”
Get full text