Non-invasive measurement of cell membrane associated proton gradients by ion-sensitive field effect transistor arrays for microphysiological and bioelectronical applications

Non-invasive measurement of cell membrane associated proton gradients by ion-sensitive field effect transistor arrays for microphysiological and bioelectronical applications

The pH in the cellular microenvironment (pH M) is an important regulator of cell-to-cell and cell-to-host interactions. Additionally the extracellular acidification rate of a cell culture is an important indicator of global cellular metabolism. In a new approach a biocompatible ion-sensitive field e...

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Bibliographic Details
Published in:Biosensors & bioelectronics Vol. 15; no. 3; pp. 117 - 124
Main Authors: Lehmann, Mirko, Baumann, Werner, Brischwein, Martin, Ehret, Ralf, Kraus, Michael, Schwinde, Anne, Bitzenhofer, Matthias, Freund, Ingo, Wolf, Bernhard
Format: Journal Article
Language:English
Published: Lausanne Elsevier B.V 01-06-2000
Elsevier Science
Subjects:
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Cancer
Cell Membrane - metabolism
Cellular sensor
Diverse techniques
Fundamental and applied biological sciences. Psychology
Glycolysis
Humans
Hydrogen-Ion Concentration
Microenvironmental pH
Molecular and cellular biology
pH-ISFET
Tumor Cells, Cultured
Glycolysis
Microenvironmental pH
Cellular sensor
Biosensors
pH-ISFET
Cancer
Cell culture
Microsensor
Biosensor
Plasma membrane
Non invasive method
pH
Proton gradient
Measurement method
Tumor cell
Ionosensible field effect transistor
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Summary:The pH in the cellular microenvironment (pH M) is an important regulator of cell-to-cell and cell-to-host interactions. Additionally the extracellular acidification rate of a cell culture is an important indicator of global cellular metabolism. In a new approach a biocompatible ion-sensitive field effect transistor (ISFET)-array was developed to measure the pH M close to a surface and the global extracellular acidification rate at the same time. This ISFET-array is part of a new multiparametric microsensor chip. The paper highlights some basic applications of this method for in-vitro measurements. Using a fluid perfusion system for cell culture media, it is possible to measure the pH M of few (five to ten) adherent tumor cells in a distance of 10–100 nm from the cell plasma membrane. Experiments showed a pH M-value of 6.68±0.06 pH. Further experiments suggest that both the low pH, and the extracellular acidification rate of the examined tumor cell line are mainly built up by glycolysis.
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ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(00)00065-8